ACRIDINE ORANGE

ACRIDINE ORANGE Properties

Melting point	284-287 °C(lit.)
Density	1.001 g/mL at 20 °C
refractive index	n20/D 1.338
storage temp.	-20°C
solubility	Soluble in water, ethanol, dimethyl sulfoxide
form	Powder
pka	-3.2, 10.5(at 25℃)
Colour Index	46005
color	orange to red
λmax	500 nm
BRN	3734978
Major Application	Adhesives; aluminophosphate crystalline materials; detecting clay particles; display device; evaluating fiber surface characteristics; glass matrixes; imaging material; inks; lasers; recording materials; photoresists; textiles; thin films; tracers for hydrology; wiring boards
Biological Applications	Detecting cancer cells,spores,human papilloma virus (HPV),stress biomarkers; treating amyloid associated diseases,lupus,pathogen infections; apoptosis assays; cytotoxicity assays
CAS DataBase Reference	65-61-2
FDA UNII	YO2175B15I
EPA Substance Registry System	C.I. Basic Orange 14 (65-61-2)

SAFETY

Risk and Safety Statements

Symbol(GHS)	GHS08
Signal word	Warning
Hazard statements	H341
Precautionary statements	P201-P202-P280-P308+P313-P405-P501
Safety Statements	22-24/25
WGK Germany	3
RTECS	AR7601000
HS Code	32041300
Toxicity	dnd-mus:ast 20 mmol/L BBACAQ 374,96,74

ACRIDINE ORANGE price More Price(28)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	318337	Acridine Orange hydrochloride hydrate 99% (HPLC)	65-61-2	1g	$146	2024-03-01	Buy
Sigma-Aldrich	318337	Acridine Orange hydrochloride hydrate 99% (HPLC)	65-61-2	5g	$714	2024-03-01	Buy
Sigma-Aldrich	A9231	Acridine Orange solution	65-61-2	10ml	$165	2024-03-01	Buy
Sigma-Aldrich	318337	Acridine Orange hydrochloride hydrate 99% (HPLC)	65-61-2	250mg	$41.5	2024-03-01	Buy
Sigma-Aldrich	A8097	Acridine Orange hydrochloride solution 10mg/mL in H	65-61-2	10ml	$106	2024-03-01	Buy

Product number	Packaging	Price	Buy
318337	1g	$146	Buy
318337	5g	$714	Buy
A9231	10ml	$165	Buy
318337	250mg	$41.5	Buy
A8097	10ml	$106	Buy

ACRIDINE ORANGE Chemical Properties,Uses,Production

Chemical Properties

dark orange to brown powder

Uses

Acridine orange is a dye that interacts with DNA and RNA by intercelation or electrostatic attraction. This cationic dye has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels.

Preparation

4,4 ‘-Bis (N, N-dimethyl) aminodiphenylmethane obtained dinitration of 4,4′ – bis (N, N-dimethyl) amino-2 ‘- dinitro-diphenyl methane reduction, and with an acid of heat, so that the diamino compound is cyclized product is oxidized and then cyclized and converted into zinc chloride double salt.

Biological Activity

acridine orange hydrochloride is a cell and organelle membrane permeable fluorescent dye that targets nucleic acids. acridine orange can either be incorporated into double helical nucleic acids (detected by green fluorescence at 530 nm), or bind electrostatically to phosphate groups of single-stranded molecules (detected by red fluorescence at 640 nm), which renders it the ability to differently stain dna and rna or single-stranded dna in situ. for unfixed cells, acridine orange staining can still be used as a simple, fast approach of obtaining information on cell cycle status and cell ploidy levels from dna staining (green fluorescence), as well as cell transcriptional activity from rna measurements (red fluorescence), in a flow cytofluorometric system. thus, acridine orange may serve as a useful cytochemical stain for cell-cycle and apoptosis studies.1. mcmaster gk, carmichael gg. analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. proceedings of the national academy of sciences of the united states of america, 1977, 74(11): 4835-4838.2. traganos f, darzynkiewicz z, sharpless t, et al. simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. journal of histochemistry and cytochemistry, 1977, 25(1): 46-56.

Safety Profile

Mutation data reported. Whenheated to decomposition it emits very toxic fumes of HCl,Cl??, and NOx.

Enzyme inhibitor

This cell-permeable dye (FWfree-base = 265.36 g/mol; CAS 65-61-2), also known as N,N,N',N'-tetramethylacridine-3,6-diamine, is an intercalator that binds tightly to nucleic acids. Binding to DNA is characterized by excitation maximum at 502 nm (cyan) and an emission maximum at 525 nm, producing a green fluorescence. (A 120 μM solution of acridine orange will detect 25-50 ng DNA per band in an agarose or polyacrylamide gel.) Binding to RNA is characterized by an excitation maximum at 460 nm and the emission maximum is located at 650 nm, yielding a reddish-orange fluorescence. Acridine orange also penetrates acidic compartments (e.g., lysosomes and phagolysosomes); upon protonation, the dye emits orange light when excited by blue light.

storage

Room temperature

Properties and Applications

bright yellow orange. Soluble in water and ethanol for orange yellow, with green fluorescent. The strong sulfuric acid in nearly colorless, a green fluorescent, diluted for orange. The dye solution with strong sodium hydroxide solution have yellow precipitation. And C.I. Solvent Orange 15 the same chemical structure (free base).

Standard( Silk )	Light Fastness	Persperation Fastness		Ironing Fastness		Soaping
Standard( Silk )	Light Fastness	Fading	Stain	Fading	Stain	Fading	Stain
ISO	1-2	3	1	5		1	3

ACRIDINE ORANGE Preparation Products And Raw materials

Raw materials

Preparation Products

Acridinium, 3,6-bis(dimethylamino)-10-methyl-, methyl sulfate

ACRIDINE ORANGE Suppliers

Global( 135)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Henan Fengda Chemical Co., Ltd	+86-371-86557731 +86-13613820652	info@fdachem.com	China	20284	58
Henan Tianfu Chemical Co.,Ltd.	+86-0371-55170693 +86-19937530512	info@tianfuchem.com	China	21634	55
Hubei Jusheng Technology Co.,Ltd.	18871490254	linda@hubeijusheng.com	CHINA	28172	58
Shaanxi Dideu Medichem Co. Ltd	+86-29-87569262 +86-15319487004	1015@dideu.com	China	3209	58
Zhengzhou Alfa Chemical Co.,Ltd	+8618530059196	sale04@alfachem.cn	China	11817	58
TargetMol Chemicals Inc.	+1-781-999-5354 +1-00000000000	marketing@targetmol.com	United States	32161	58
GERBU Biotechnik GmbH	+49-62217264160 +49-62217264160	found@gerbu.de	Germany	4	58
Nantong HI-FUTURE Biology Co., Ltd.	+undefined18051384581	sales@chemhifuture.com	China	3135	58
TargetMol Chemicals Inc.		support@targetmol.com	United States	38631	58
GIHI CHEMICALS CO.,LIMITED	+8618058761490	info@gihichemicals.com	China	49978	58

Supplier	Advantage
Henan Fengda Chemical Co., Ltd	58
Henan Tianfu Chemical Co.,Ltd.	55
Hubei Jusheng Technology Co.,Ltd.	58
Shaanxi Dideu Medichem Co. Ltd	58
Zhengzhou Alfa Chemical Co.,Ltd	58
TargetMol Chemicals Inc.	58
GERBU Biotechnik GmbH	58
Nantong HI-FUTURE Biology Co., Ltd.	58
TargetMol Chemicals Inc.	58
GIHI CHEMICALS CO.,LIMITED	58

View Lastest Price from ACRIDINE ORANGE manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2024-11-19	Acridine Orange hydrochloride 65-61-2	US $47.00 / mg		99.3%	10g	TargetMol Chemicals Inc.
2024-11-19	Acridine Orange hydrochloride 65-61-2	US $47.00 / mg		99.3%	10g	TargetMol Chemicals Inc.
2024-03-26	ACRIDINE ORANGE 65-61-2	US $5.00-0.20 / KG	1KG	98%	g-kg-tons, free sample is available	Henan Fengda Chemical Co., Ltd

Acridine Orange hydrochloride
65-61-2
US $47.00 / mg
99.3%
TargetMol Chemicals Inc.

Acridine Orange hydrochloride
65-61-2
US $47.00 / mg
99.3%
TargetMol Chemicals Inc.

ACRIDINE ORANGE
65-61-2
US $5.00-0.20 / KG
98%
Henan Fengda Chemical Co., Ltd

ACRIDINE ORANGE Spectrum

ACRIDINE ORANGE(65-61-2)¹HNMR ACRIDINE ORANGE(65-61-2)IR

65-61-2(ACRIDINE ORANGE)Related Search:

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