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CALCEIN-AM

CALCEIN-AM structure

CAS No.: 148504-34-1

Chemical Name:: CALCEIN-AM

Synonyms: CAL-AM;NSC 689290;CALCEIN-AM;AM /Calcein-AM;CALCEIN, AM;CAL-AM;Calcein AM solution;Cellstain- Calcein-AM;Calcein AcetoxyMethyl Ester;-Cellstain-Calcein-AmSolution;Calcein, AM *CAS 148504-34-1*

CBNumber:: CB6304173

Molecular Formula:: C46H46N2O23

Molecular Weight:: 994.86

MDL Number:: MFCD28355895

MOL File:: 148504-34-1.mol

MSDS File:: SDS

Last updated:2024-11-18 13:24:21

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CALCEIN-AM Properties

Melting point	>250℃
Boiling point	982.7±65.0 °C(Predicted)
Density	1.49±0.1 g/cm3(Predicted)
refractive index	n20/D 1.479
Flash point	85 °C
storage temp.	-20°C
solubility	DMSO: soluble10mg/mL, clear, colorless to slightly yellow
pka	2.66±0.50(Predicted)
form	powder
color	Colorless
λmax	< 300 nm
Biological Applications	Calcium indicators; zinc indicators; cytotoxicity assays; apoptosis assays; viability assays; labile iron pool assays; chemotaxis probes; cell adhesion probes; mitochondrial probes; P-glycoprotein probes;multi-drug resistance probes;treating atherosclerosis,cancer;ischemic disease

SAFETY

Risk and Safety Statements

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NA 1993 / PGIII
WGK Germany	3
F	10-21

CALCEIN-AM price More Price(14)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	C1359	Calcein AM solution 4?mM in DMSO, ≥90% (HPLC), solution	148504-34-1	100μL	$327	2024-03-01	Buy
Sigma-Aldrich	56496	Calcein-AM Small Package (20 X 50 μg ), ≥96.0% (HPLC)	148504-34-1	20x50μg	$905	2024-03-01	Buy
Sigma-Aldrich	17783	Calcein-AM BioReagent, suitable for fluorescence, ≥96.0% (HPLC)	148504-34-1	1mg	$568	2024-03-01	Buy
Sigma-Aldrich	17783	Calcein-AM BioReagent, suitable for fluorescence, ≥96.0% (HPLC)	148504-34-1	5mg	$2460	2024-03-01	Buy
Cayman Chemical	14948	Calcein AM ≥90%	148504-34-1	500μg	$73	2024-03-01	Buy

Product number	Packaging	Price	Buy
C1359	100μL	$327	Buy
56496	20x50μg	$905	Buy
17783	1mg	$568	Buy
17783	5mg	$2460	Buy
14948	500μg	$73	Buy

CALCEIN-AM Chemical Properties,Uses,Production

Chemical Properties

A colorless to slightly yellow solid. Soluble in dimethyl sulfoxide.

Uses

A non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule

Uses

Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. Calcein-AM can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP.

Uses

Calcein-AM appears to best satisfy the criteria for assaying cell adhesion and to have the least effect on cell viability and cell functions.

Definition

ChEBI: Calcein am is an organic heteropentacyclic compound that is calcein in which all four carboxy group hydrogens have been substituted by (acetyloxy)methoxy groups and the hyrodgens of the two hydroxy groups have been substituted by acetyl groups. It is a a non-fluorescent probe cleaved to a fluorescent probe by non-specific intracellular esterases. It has a role as a fluorochrome. It is an acetate ester, an organic heteropentacyclic compound, a gamma-lactone, an oxaspiro compound, a member of 2-benzofurans and a xanthene dye. It is functionally related to a calcein.

General Description

Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

Biochem/physiol Actions

Fluorescent cell permeable derivative of calcein.

storage

-20°C

References

[1] JAMES HYNES . Fluorescence-Based Cell Viability Screening Assays Using Water-Soluble Oxygen Probes[J]. SLAS Discovery, 2003, 8 3: Pages 264-272. DOI:10.1177/1087057103008003004.
[2] SABNIS R W. Handbook of Biological Dyes and Stains: Synthesis and Industrial Applications[C]. 2010: 0. DOI:10.1002/9780470586242.
[3] Polli, J.W., Wring, S.A., Humphreys, J.E., et al. Rational use of in vitro P-glycoprotein assays in drug discovery. J. Pharmacol. Exp. Ther. 299(2), 620-628 (2001).
[4] Tiberghien, F., and Loor, F. Ranking of P-glycoprotein substates and inhibitors by a calcein-AM fluorometry screening assay. Anticancer Drugs 7(5), 568-578 (1996).
[5] Szakács, G., Jakab, K., Antal, F., et al. Diagnostics of multidrug resistance in cancer. Pathol.Oncol.Res. 4(4), 251-257 (2016).
[6] X M WANG. A new microcellular cytotoxicity test based on calcein AM release.[J]. Human Immunology, 1993, 37 4: 264-270. DOI:10.1016/0198-8859(93)90510-8.
[7] ZSOLT HOLLó . Calcein accumulation as a fluorometric functional assay of the multidrug transporter[J]. Biochimica et biophysica acta. Biomembranes, 1994, 1191 2: Pages 384-388. DOI:10.1016/0005-2736(94)90190-2.

CALCEIN-AM Preparation Products And Raw materials

Raw materials

Preparation Products

CALCEIN-AM Suppliers

Global( 132)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Shanghai Zheyan Biotech Co., Ltd.	18017610038	zheyansh@163.com	CHINA	3619	58
CONIER CHEM AND PHARMA LIMITED	+8618523575427	sales@conier.com	China	49374	58
TargetMol Chemicals Inc.	+1-781-999-5354 +1-00000000000	marketing@targetmol.com	United States	32165	58
Hubei Ipure Biology Co., Ltd	+8613367258412	ada@ipurechemical.com	China	10319	58
HONG KONG IPURE BIOLOGY CO.,LIMITED	86 18062405514 18062405514	ada@ipurechemical.com	CHINA	3461	58
Alfa Chemistry		Info@alfa-chemistry.com	United States	24072	58
Aromsyn Co., Ltd.	+86-0571-85585865 +8613336024896	CB@aromsyn.com	China	19183	58
Hebei Weibang Biotechnology Co., Ltd	+8617732866630	bess@weibangbio.com	China	18154	58
ZHEJIANG JIUZHOU CHEM CO., LTD	+86-0576225566889 +86-13454675544	admin@jiuzhou-chem.com；jamie@jiuzhou-chem.com；alice@jiuzhou-chem.com	China	12272	58
Wuhan Topule Biopharmaceutical Co., Ltd	+8618327326525	masar@topule.com	China	8467	58

Supplier	Advantage
Shanghai Zheyan Biotech Co., Ltd.	58
CONIER CHEM AND PHARMA LIMITED	58
TargetMol Chemicals Inc.	58
Hubei Ipure Biology Co., Ltd	58
HONG KONG IPURE BIOLOGY CO.,LIMITED	58
Alfa Chemistry	58
Aromsyn Co., Ltd.	58
Hebei Weibang Biotechnology Co., Ltd	58
ZHEJIANG JIUZHOU CHEM CO., LTD	58
Wuhan Topule Biopharmaceutical Co., Ltd	58

View Lastest Price from CALCEIN-AM manufacturers

Image	Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
	2024-10-24	Calcein-AM 148504-34-1	US $345.00 / mg			10g	TargetMol Chemicals Inc.

Calcein-AM
148504-34-1
US $345.00 / mg
TargetMol Chemicals Inc.

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3',6'-Di(O-acetyl)-2',7'-bis[N,N-bis(carboxymethyl)aminomethyl]fluoresceintetraacetoxymethylester,DMSOsolution Calcein AM solution CALCEIN, AM;CAL-AM 4',5'-Bis(N,N-bis(carboxymethyl)aminomethyl)fluorescein acetoxymethyl ester Glycine, N,N'-((3',6'-bis(acetyloxy)-3-oxospiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-diyl)bis(methylene))bis(N-(2-((acetyloxy)methoxy)-2-oxoethyl)-, bis((acetyloxy)methyl) ester Calcein AcetoxyMethyl Ester N,N'-[[3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(Methylene)]bis[N-[2-[(acetyloxy)Methoxy]-2-oxoethyl]glycine 1,1'-Bis[(acetyloxy) Methyl] Ester NSC 689290 CALCEIN-ACETOXYMETHYL ESTER, DIACETATE CALCEIN-AM CAL-AM 3',6'-DI(O-ACETYL)-2',7'-BIS(N,N-BIS(CARBOXYMETHYL)-AMINOMETHYL)FLUORESCEIN-TETRAACETOXYMETHYL ESTER Cellstain- Calcein-AM CALCEIN-AM SOLUTION, 1 MMOL IN DMSO* CALCEIN AM, 4MM IN ANHYDROUS DMSO -Cellstain-Calcein-AmSolution calcein o,o'-diacetate tetrakis(acetoxymethyl)ester Calcein O,o0-diacetate Tetrakis(acetoxymethyl) ester Glycine, N,n0-[[30,60-bis(acetyloxy)-3-oxospiro[ isobenzofuran-1(3h),90-[9h]xanthene]-20,70-diyl] bis(methylene)]bis[n-[2-[(acetyloxy)methoxy]-2-oxoethyl]-, bis[(acetyloxy)methyl] Ester Calcein, AM *CAS 148504-34-1* Calcein, AM *UltraPure grade* *CAS 148504-34-1* Calcein AM (Calcein acetoxymethyl ester) Calcein-AM - CAS 148504-34-1 - Calbiochem Cellstain Calcein AM Solution(1mM/l DMSO) (3',6'-Di(O-acetyl)-4',5'-bis[N,N-bis(carboxymethy Calcein-AM (3',6'-Di(O-acetyl)-2',7'-bis[N,N-bis(carboxymethyl)aminomethyl]fluoresceinTetraacetoxymethyl Ester) Glycine, N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-, 1,1'-bis[(acetyloxy)methyl] ester AM /Calcein-AM Tetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-2',7'-diyl)bis(methylene))bis(azanetriyl))tetraacetate Tetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-2',7'-diyl)bis(methylene))bis(azanetriyl))tetraacetate 148504-34-1 C46H46N2O23 Amines Aromatics Fluorescent Labels & Indicators Heterocycles Intermediates & Fine Chemicals Pharmaceuticals Xanthene CCalcium Signaling Research Tools Calcium Probes Fluorescent Stains and Dyes Stains&Dyes, A to