Adenosine 5'-diphosphoribose sodium salt

Description

Adenosine 5′-diphosphoribose sodium (ADP ribose sodium) is a nicotinamide adenine nucleotide (NAD+) metabolite. It is the most potent and primary intracellular Ca2+-permeable cation TRPM2 channel activator and can also enhance autophagy.

Uses

5′-Deoxy-5′-(methylthio)adenosine has been used:

• to give a range of nicotinamide adenine dinucleotide (NADH)
• oxidation rates for the respiratory complex (CI/CIII/CIV)
• CI/alternative(AOX) pathway
• to measure poly(ADP-ribose) glycohydrolaseactivity
• poly(ADP-ribose) (PAR) binding competition assay
• oxidase?(AOX) pathway
• to measure poly(ADP-ribose) glycohydrolase activity
• poly(ADP-ribose) (PAR) binding competition assay

Biochem/physiol Actions

ADP-Ribose is used as the basic building block used as a substrate in the formation of poly(ADP-ribose) by members of the poly(ADP-ribose) polymerase (PARP) family. Poly(ADP-ribose) formation and degradation are an important components of DNA repair and apoptosis signaling.

in vitro

In mouse embryonic fibroblasts (MEFs), H2O2 treatment demonstrates that the activation of poly(ADP-ribose) (PAR) polymerase-1 (PARP-1) produced Adenosine 5′-diphosphoribose (ADP ribose), which is an activating signal for TRPM2 channels, thereby promoting Ca2+ elevation through extracellular Ca2+ influx and (or) lysosomal Ca2+ release. This process eventually activates early or late autophagy in response to different degrees of oxidative stress.
TRPM2 channels are activated by binding Adenosine 5′-diphosphoribose (ADP ribose) to the intracellular NUDT9-homology (NUDT9-H) domain unique to TRPM2 and located at its C terminus. In addition to ADPR, intracellular Ca2+ is an essential coactivator: TRPM2 channels open only in the combined presence of both ligands.