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Asunaprevir

CAS No.
630420-16-5
Chemical Name:
Asunaprevir
Synonyms
CS-1413;BMS 650032;Asunaprevir;Asunaprevir API;Asunaprevir,≥98%;Asunaprevir USP/EP/BP;BMS 650032/Asunaprevir;Asunaprevir (BMS-650032);Asunaprevir (10mM in DMSO);BMS-650032; BMS 650032; BMS650032
CBNumber:
CB92614356
Molecular Formula:
C35H46ClN5O9S
Molecular Weight:
748.29
MDL Number:
MFCD27987900
MOL File:
630420-16-5.mol
Last updated:2023-06-30 15:45:59

Asunaprevir Properties

Melting point 149-150oC
Density 1.37
storage temp. -20°C Freezer, Under inert atmosphere
solubility DMSO (Slightly), Methanol (Slightly, Heated)
form Solid
pka 4.49±0.40(Predicted)
color White to Off-White
FDA UNII S9X0KRJ00S
NCI Drug Dictionary asunaprevir
ATC code J05AP06

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS07
Signal word  Warning
Hazard statements  H302-H315-H319-H335
Precautionary statements  P261-P305+P351+P338

Asunaprevir price More Price(22)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Cayman Chemical 20835 Asunaprevir ≥98% 630420-16-5 1mg $81 2024-03-01 Buy
Cayman Chemical 20835 Asunaprevir ≥98% 630420-16-5 5mg $356 2024-03-01 Buy
Cayman Chemical 20835 Asunaprevir ≥98% 630420-16-5 10mg $632 2024-03-01 Buy
Cayman Chemical 20835 Asunaprevir ≥98% 630420-16-5 25mg $1184 2024-03-01 Buy
TRC A788753 Asunaprevir 630420-16-5 1mg $60 2021-12-16 Buy
Product number Packaging Price Buy
20835 1mg $81 Buy
20835 5mg $356 Buy
20835 10mg $632 Buy
20835 25mg $1184 Buy
A788753 1mg $60 Buy

Asunaprevir Chemical Properties,Uses,Production

Description

Sold under the trade name Sunvepra®, asunaprevir received approval in Japan as part of a combination treatment for the hepatitis C virus (HCV). Working in concert with daclatasvir (IX) (vide infra), asunaprevir is a unique treatment for HCV, as it is free from both interferon and ribavirin and is administered orally. This direct-acting anti-viral, which was developed by Bristol–Myers Squibb (BMS), works as an NS3/4A protease inhibitor, representing a valuable treatment option for patient populations who are unable to receive, or do not respond to, the standard course of treatment—peginterferon/ribavirin.

Description

Asunaprevir is an inhibitor of the hepatitis C non-structural 3/4A serine protease, which is required for viral replication. It is a direct-acting inhibitor of hepatitis C virus (HCV). It is commonly used in combination with daclatasvir and beclabuvir, which are also direct-acting HCV inhibitors.

Uses

Asunaprevir is an inhibitor of the viral enzyme serine protease HCV NS3. Also functions as a second generation NS3/4A protease inhibitor used in the treatment of hepatitis C.

Definition

ChEBI: Asunaprevir is an oligopeptide.

Synthesis

The preparation of chloroquinoline 27 began with bromination of commercially available acetophenone 20, which was carefully carried out on over 1 kg scale in a reactor equipped with an HBr scrubbing mechanism (bubbler outfitted) to furnish a-bromoketone 21. Next, nucleophilic displacement of the bromide using sodium diformamide (22) under phase transfer conditions led to the putative intermediate 23. This system underwent in situ deprotection of the diformamide functionality followed by cyclization under mildly acidic conditions to arrive at isoquinolone 24 in 78% yield across the two-step, one-pot sequence. Next, methylation of the hydroxyl group at C-4 was carried out using methanolic methanesulfonic acid at elevated temperatures followed by treatment with aqueous ammonium hydroxide to quench any excess acid. Subsequent treatment with phosphorous oxychloride furnished dichloroisoquinoline 26 in good yield. A nucleophilic aromatic substitution reaction employing potassium t-butoxide was used to establish an arylpyrrolidino ether linkage and, upon workup with mildly acidic conditions, the proline derivative 27 emerged in 59% yield.
Synthesis_630420-16-5
Although the synthesis of asunaprevir vinyl cyclopropane subunit 35 has been described by researchers at BMS, it is interesting to note that this subunit, or structural derivatives of 35, have been found within a number of other antiviral drugs, particularly those which target inhibition of NS3 protease as its mechanism of action. For example, researchers at Boehringer-Ingelheim have described a scale synthesis of (1R,2S)-1-amino-2-vinylcyclopropane carboxylic acid (des-N-tert-butoxycarbonyl (Boc) 32; also referred to as ??vinyl-ACCA?ˉ) as a means to incorporate this subunit into that company?ˉs antiviral agent BILN 2061. A macrocyclic version of this same cyclopropane-containing system is found in the antiviral agents paritaprevir hydrate (XXVII) developed by Enanta and Abbvie, and a saturated version in vaniprevir (XXXVII) developed by Merck and described later in this review (vide infra). The preparation of 32 described by Beaulieu and co-workers was performed on pilot-plant scale and the details surrounding the conversion of 32 to 35 are described by the BMS patent. Beginning with commercially available methyl glycine HCl (28), condensation with benzaldehyde in the presence of a dehydrating reagent (trimethyl orthoformate) and base led to the transient imine 29. This underwent a highly diastereoselective alkylation reaction whereby treatment with lithium t-butoxide followed by subjection to 1,4- dibromo-2-butene facilitated an SN2¨CSN20 reaction. Subsequent acidification and Boc protection resulted in 30-rac as a racemate that existed as a single diastereomer having the vinyl and the ester groups in a cis configuration. The authors postulate that the lithium enolate intermediate which forms upon the initial alkylation binds the proximal bromine atom which accounts for the stereoselectivity of the reaction. Next, selective saponification of the undesired enantiomer 31b took place via treatment with acalase 2.4 L, which is a remarkably versatile and relatively inexpensive enzyme capable of resolving a wide variety of racemic esters,49 under basic conditions. This enzymatic resolution returned a 49% yield of the desired ester 31a (theoretical 50%), and a simple aqueous workup removed the undesired acid 31b. Methyl ester 31a was then saponified using methanolic lithium hydroxide to give 32, which was subsequently coupled with cyclopropanesulfonamide 33 to deliver 34 in excellent yield. Next, removal of the Boc group using TFA followed by salt formation with ethereal HCl delivered the key cyclopropane subunit 35.
QQ??í?20210203163951.jpg
Cyclopropyl amine 35 was subjected to proline derivative 27 under conventional amide bond-forming conditions. This was followed by removal of the pyrrolidine nitrogen Boc group with acid and subsequent coupling with N-Boc-3-methyl valine (37) to deliver asunaprevir (IV) in good yield for each of these steps.
QQ??í?20210203164011.jpg

Enzyme inhibitor

This highly selective HCV antiviral (FW = 748.29 g/mol; CAS 630420-16- 5; Symbol: ASV), also named BMS-650032, targets NS3 protease, a serine proteinase required for Hepatitis C Virus (HCV) polyprotein processing, showing good antiviral activity against replicons based on HCV Genotype 1a (EC50 = 4 nM), Genotype 1b (EC50 = 1.2 nM), Genotype 4 (EC50 = 1.8 nM), Genotype 5 (EC50 = 1.7 nM), and Genotype 6 (EC50 = 0.9 nM). It is far less effective against Genotype 2 (EC50 = 67 nM) and Genotype 3 (EC50 = >1100 nM). Asunaprevir is a peptidomimetic that occupies the active site, inhibiting the HCV NS3/4A serine protease, with little or no action against related viruses. The average Ki for ASV is approximately 0.4 nM and 0.2 nM for Genotype 1a and Genotype 1b, respectively. Its acylsulfonamide moeity interacts noncovalently with the NS3 protease – unlike telaprevir’s α-ketoamide moiety, which is linked covalently to NS3/4A’s catalytic serine and reverses slowly over time. ASV exhibits an excellent selectivity index (>40,000x) against all serine/cysteine proteases evaluated, including human leukocyte elastase, porcine pancreatic elastase, and three members of the chymotrypsin family. Although ASV shows a low barrier to resistance, it can be combined with daclatasvir to achieve a very high rate of viral eradication in both na?ve and treatment-experienced patients, showing a sustained virological response rate of 80%-90%.

storage

Store at -20°C

References

[1] scola pm, sun lq, wang ax, chen j, sin n, venables bl, sit sy, chen y, cocuzza a, bilder dm, d'andrea sv, zheng b, hewawasam p, tu y, friborg j, falk p, hernandez d, levine s, chen c, yu f, sheaffer ak, zhai g, barry d, knipe jo, han yh, schartman r, donoso m, mosure k, sinz mw, zvyaga t, good ac, rajamani r, kish k, tredup j, klei he, gao q, mueller l, colonno rj, grasela dm, adams sp, loy j, levesque pc, sun h, shi h, sun l, warner w, li d, zhu j, meanwell na, mcphee f. the discovery of asunaprevir (bms-650032), an orally efficacious ns3 protease inhibitor for the treatment of hepatitis c virus infection. j med chem. 2014 mar 13;57(5):1730-52.
[2] mcphee f, sheaffer ak, friborg j, hernandez d, falk p, zhai g, levine s, chaniewski s, yu f, barry d, chen c, lee ms, mosure k, sun lq, sinz m, meanwell na, colonno rj, knipe j, scola p. preclinical profile and characterization of the hepatitis c virus ns3 protease inhibitor asunaprevir (bms-650032). antimicrob agents chemother. 2012 oct;56(10):5387-96.

1169846-35-8
62965-35-9
630420-16-5
Synthesis of Asunaprevir from (2S,4R)-4-((7-chloro-4-Methoxyisoquinolin-1-yl)oxy)-N-((1R,2S)-1-((cyclopropylsulfonyl)carbaMoyl)-2-vinylcyclopropyl)pyrrolidine-2-carboxaMide hydrochloride and N-Boc-L-tert-Leucine
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View Lastest Price from Asunaprevir manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Asunaprevir pictures 2024-11-29 Asunaprevir
630420-16-5
US $0.00 / g/Bag 1g 98%-102% 100G WUHAN FORTUNA CHEMICAL CO., LTD
Asunaprevir pictures 2022-09-08 Asunaprevir
630420-16-5
US $0.00-0.00 / kg 1kg 98% 1Ton Henan Aochuang Chemical Co.,Ltd.
Asunaprevir pictures 2021-11-25 Asunaprevir
630420-16-5
US $15.00 / g 1KG 99% 5ton/Month Qiuxian Baitai New Material Co., LTD
  • Asunaprevir pictures
  • Asunaprevir
    630420-16-5
  • US $0.00 / g/Bag
  • 98%-102%
  • WUHAN FORTUNA CHEMICAL CO., LTD
  • Asunaprevir pictures
  • Asunaprevir
    630420-16-5
  • US $0.00-0.00 / kg
  • 98%
  • Henan Aochuang Chemical Co.,Ltd.
  • Asunaprevir pictures
  • Asunaprevir
    630420-16-5
  • US $15.00 / g
  • 99%
  • Qiuxian Baitai New Material Co., LTD

Asunaprevir Spectrum

(1R,2S)-N-[(1,1-Dimethylethoxy)carbonyl]-3-methyl-L-valyl-(4R)-4-[(7-chloro-4-methoxy-1-isoquinolinyl)oxy]-L-prolyl-1-amino-N-(cyclopropylsulfonyl)-2-ethenylcyclopropanecarboxamide Asunaprevir BMS 650032 Asunaprevir (BMS-650032) BMS 650032/Asunaprevir Asunaprevir API tert-butyl N-[(2S)-1-[(2S,4R)-4-(7-chloro-4-methoxyisoquinolin-1-yl)oxy-2-[[(1R,2S)-1-(cyclopropylsulfonylcarbamoyl)-2-ethenylcyclopropyl]carbamoyl]pyrrolidin-1-yl]-3,3-dimethyl-1-oxobutan-2-yl]carbamate BMS-650032; BMS 650032; BMS650032 CS-1413 tert-Butyl ((2S)-1-((2S)-2-(((1R,2S)-1-amino-2-vinylcyclopropane-1-carbonyl)(cyclopropylsulfonyl)carbamoyl)-4-((7-chloro-4-methoxyisoquinolin-1-yl)oxy)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)carbamate Asunaprevir,≥98% Cyclopropanecarboxamide, N-[(1,1-dimethylethoxy)carbonyl]-3-methyl-L-valyl-(4R)-4-[(7-chloro-4-methoxy-1-isoquinolinyl)oxy]-L-prolyl-1-amino-N-(cyclopropylsulfonyl)-2-ethenyl-, (1R,2S)- Asunaprevir USP/EP/BP Asunaprevir (10mM in DMSO) tert-Butyl ((S)-1-((2S,4R)-4-((7-chloro-4-methoxyisoquinolin-1-yl)oxy)-2-(((1R,2S)-1-((cyclopropylsulfonyl)carbamoyl)-2-vinylcyclopropyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)carbama 630420-16-5 C35H46ClN5O9S API