Alkaline Phosphatase
- CAS No.
- 9001-78-9
- Chemical Name:
- Alkaline Phosphatase
- Synonyms
- AP;alkaline;MCAP;CIAP;AKP;IRAP;IL1F3;HUNK1;HUNKI;Apase
- CBNumber:
- CB9690084
- Molecular Formula:
- C21H36N8O6
- Molecular Weight:
- 496.56054
- MDL Number:
- MFCD00131849
- MOL File:
- 9001-78-9.mol
- MSDS File:
- SDS
| Density | 1.12 g/mL at 20 °C |
|---|---|
| storage temp. | -20°C |
| form | suspension |
| color | white |
| Specific Gravity | 1 |
| Water Solubility | It is soluble in water. |
| Stability | Stability Incompatible with strong oxidizing agents |
| EWG's Food Scores | 1 |
| EPA Substance Registry System | Alkaline phosphatase (9001-78-9) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|---|---|
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P280 |
| Hazard Codes | B,Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36-24/25-22-23 |
| WGK Germany | 3 |
| F | 3-10 |
| TSCA | Yes |
| HS Code | 35079090 |
| Toxicity | An enzyme with an alkaline pH optimum that hydrolyzes phosphate monoesters. Elevation of its activity in the serum usually indicates obstructive jaundice, Paget’s disease (osteitis deformans), or bone carcinoma. |
Alkaline Phosphatase price More Price(51)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | SAE0111 | Phosphatase, Alkaline from human placenta ≥10?units/mg solid (in glycine buffer) | 9001-78-9 | 100UNITS | $147 | 2024-03-01 | Buy |
| Sigma-Aldrich | SRP3327 | IL-1 RECEPTOR ANTAGONIST human Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC) | 100μG | $412 | 2024-03-01 | Buy | |
| Sigma-Aldrich | SAE0111 | Phosphatase, Alkaline from human placenta ≥10?units/mg solid (in glycine buffer) | 9001-78-9 | 1000UNITS | $611 | 2024-03-01 | Buy |
| Sigma-Aldrich | P8361 | Phosphatase, Alkaline bovine recombinant, expressed in Pichia pastoris, ≥4000?units/mg protein | 9001-78-9 | 5000units | $521 | 2024-03-01 | Buy |
| Sigma-Aldrich | P7923 | Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous glycerol solution, ≥4,000?DEA units/mg protein | 9001-78-9 | 2000units | $178 | 2024-03-01 | Buy |
Alkaline Phosphatase Chemical Properties,Uses,Production
Chemical Properties
solid
Uses
Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions.
Uses
Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.
Uses
Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids.
General Description
Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.
Biochem/physiol Actions
Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).
Purification Methods
The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.]
Alkaline Phosphatase Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Shaanxi Dideu Medichem Co. Ltd | +86-29-81148696 +86-15536356810 | 1022@dideu.com | China | 3882 | 58 |
| Hangzhou ICH Biofarm Co., Ltd | +86-0571-28186870; +undefined8613073685410 | sales@ichemie.com | China | 1001 | 58 |
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 | info@tianfuchem.com | China | 21634 | 55 |
| career henan chemical co | +86-0371-86658258 +8613203830695 | sales@coreychem.com | China | 29880 | 58 |
| SHANDONG ZHI SHANG CHEMICAL CO.LTD | +86 18953170293 | sales@sdzschem.com | China | 2930 | 58 |
| Hubei xin bonus chemical co. LTD | 86-13657291602 | linda@hubeijusheng.com | CHINA | 22963 | 58 |
| Chongqing Chemdad Co., Ltd | +86-023-6139-8061 +86-86-13650506873 | sales@chemdad.com | China | 39894 | 58 |
| Shaanxi Dideu Medichem Co. Ltd | 18192627656 | 1012@dideu.com | China | 3003 | 58 |
| Creative Enzymes | 1-516-855-7709 | info@creative-enzymes.com | United States | 8748 | 58 |
| TargetMol Chemicals Inc. | +1-781-999-5354 +1-00000000000 | marketing@targetmol.com | United States | 32161 | 58 |
View Lastest Price from Alkaline Phosphatase manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2024-11-19 | Alkaline Phosphatase
9001-78-9
|
US $39.00-128.00 / mg | 10g | TargetMol Chemicals Inc. | |||
 |
2024-08-30 | Alkaline Phosphatase
9001-78-9
|
US $0.10 / KG | 1KG | 99.0% | 1000 tons | Shaanxi Dideu Medichem Co. Ltd | |
 |
2023-11-01 | Alkaline Phosphatase
9001-78-9
|
US $0.00-0.00 / g | 1g | 99% HPLC | 100kg | Hangzhou ICH Biofarm Co., Ltd |
-
- Alkaline Phosphatase
9001-78-9
- US $39.00-128.00 / mg
- TargetMol Chemicals Inc.
-
- Alkaline Phosphatase
9001-78-9
- US $0.10 / KG
- 99.0%
- Shaanxi Dideu Medichem Co. Ltd
-
- Alkaline Phosphatase
9001-78-9
- US $0.00-0.00 / g
- 99% HPLC
- Hangzhou ICH Biofarm Co., Ltd
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