SPH I
- CAS No.
- 85270-15-1
- Chemical Name:
- SPH I
- Synonyms
- APA I;SPH I;Einecs 286-569-8;APA I RESTRICTION ENZYME;SPH I RESTRICTION ENZYME;restriction endonuclease Sph I;RESTRICTION ENDONUCLEASE APA I;RESTRICTION ENDONUCLEASES APA I;RESTRICTION ENDONUCLEASES SPH I;apa i from acetobacter pasteurianus
- CBNumber:
- CB0280961
- Molecular Formula:
- Molecular Weight:
- 0
- MOL File:
- Mol file
- Modify Date:
- 2023/4/23 13:52:06
SPH I Chemical Properties,Uses,Production
General Description
Compatible ends
Apa I ends are not compatible with those generated by any other known restriction enzymes.
Isoschizomers
Apa I is an isoschizomer to Bsp 120 I and Psp OMI.
Methylation sensitivity
Apa I is inhibited by 5′-methylcytosine at the sites indicated (*) in the recognition sequence.
Activity in SuRE/Cut Buffer System
Buffer printed in bold face type is the buffer recommended for optimal activity:
ABLMH
100%10-25%50-75%50-75%0-10%
Relative activity in complete PCR mix
Relative activity in PCR mix (Taq DNA Polymerase buffer) is 100%. The PCR mix contained target DNA, primers, 10 mM Tris-HCl (pH 8.3, +20°C), 50 mM KCl, 1.5 mM MgCl2, 200 μM dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles. Activity in reaction buffer of Pwo SuperYield DNA Polymerase PCR Mix is 10%. When supplemented with GC-RICH Solution activity is increased to >100%.
Incubation temperature
+30°C
Unit definition
One unit is the enzyme activity that completely cleaves 1 μg ? × Hind III fragments in 1 hour at +30°C in a total volume of 25 μl SuRE/Cut Buffer A.
Heat inactivation
The enzyme can be heat inactivated by incubating it for 15 minutes at +65°C.
Number of cleavage sites on different DNAs
λAd2SV40φ X174M13mp7M13mp18pBR322pBR328pUC18
112 1000000
Ligation and recutting assay
Apa I fragments obtained by complete digestion of 1 μg λ × Hind III fragments are ligated with 1 U T4 DNA Ligase in a volume of 10 μl by incubation for 16 hours at +4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at +20°C) resulting in >95% recovery of 1 μg λ × Hind III fragments.
Subsequent re-cutting with Apa I yields >95% of the typical pattern of λ × Hind III× Apa I fragments.
SPH I Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | Inquiry |
|---|---|---|---|---|---|
| Career Henan Chemica Co | +86-0371-86658258 +8613203830695 | China | 30253 | 58 | Inquiry |
| Shanghai Mengze Biotechnology Co., Ltd. | 13817203524 | CHINA | 6393 | 58 | Inquiry |
| Sigma-Aldrich | 021-61415566 800-8193336 | China | 51471 | 80 | Inquiry |
| Creative Enzymes | 1-516-855-7709 | United States | 6057 | 58 | Inquiry |
| Beyotime Biotechnology | 021-67602129 | CHINA | 4658 | 58 | Inquiry |
| Jena Bioscience GmbH | +49 3641 46 49 52 | Germany | 335 | 81 | Inquiry |
| Qbiogene | 800 424 6101 | United States | 925 | 69 | Inquiry |
| Amersham Biosciences, Inc. | 1 800 526 3593 | United States | 1270 | 85 | Inquiry |
| FERMENTAS Inc. | 1 800 340 9026 | United States | 335 | 82 | Inquiry |
| Nacalai Tesque, Inc. | 81 75 251 1723 | Japan | 6048 | 75 | Inquiry |
| Supplier | Advantage |
|---|---|
| Career Henan Chemica Co | 58 |
| Shanghai Mengze Biotechnology Co., Ltd. | 58 |
| Sigma-Aldrich | 80 |
| Creative Enzymes | 58 |
| Beyotime Biotechnology | 58 |
| Jena Bioscience GmbH | 81 |
| Qbiogene | 69 |
| Amersham Biosciences, Inc. | 85 |
| FERMENTAS Inc. | 82 |
| Nacalai Tesque, Inc. | 75 |
85270-15-1(SPH I)Related Search:
1of4
chevron_right