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ACRIDINE ORANGE

ACRIDINE ORANGE Structure
CAS No.
65-61-2
Chemical Name:
ACRIDINE ORANGE
Synonyms
AO;AOX1;Lysotraker;CI NO 46005;BASIC ORANGE;-Cellstain-Ao;Euchrysine 3r;EUCHRYSINE 3RX;acridineoranger;RHODULINE ORANGE
CBNumber:
CB0747468
Molecular Formula:
C17H20ClN3
Molecular Weight:
301.82
MOL File:
65-61-2.mol
Modify Date:
2024/3/26 18:04:44

ACRIDINE ORANGE Properties

Melting point 284-287 °C(lit.)
Density 1.001 g/mL at 20 °C
refractive index n20/D 1.338
storage temp. -20°C
solubility Soluble in water, ethanol, dimethyl sulfoxide
form Powder
pka -3.2, 10.5(at 25℃)
Colour Index 46005
color orange to red
λmax 500 nm
BRN 3734978
Biological Applications Detecting cancer cells,spores,human papilloma virus (HPV),stress biomarkers; treating amyloid associated diseases,lupus,pathogen infections; apoptosis assays; cytotoxicity assays
Major Application Adhesives; aluminophosphate crystalline materials; detecting clay particles; display device; evaluating fiber surface characteristics; glass matrixes; imaging material; inks; lasers; recording materials; photoresists; textiles; thin films; tracers for hydrology; wiring boards
CAS DataBase Reference 65-61-2
EPA Substance Registry System C.I. Basic Orange 14 (65-61-2)

SAFETY

Risk and Safety Statements

Symbol(GHS) 
GHS08
Signal word  Warning
Hazard statements  H341
Precautionary statements  P201-P202-P280-P308+P313-P405-P501
Safety Statements  22-24/25
WGK Germany  3
RTECS  AR7601000
HS Code  32041300
Toxicity dnd-mus:ast 20 mmol/L BBACAQ 374,96,74

ACRIDINE ORANGE price More Price(12)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich(India) 318337 Acridine Orange hydrochloride hydrate ≥98% (HPLC) 65-61-2 250MG ₹2985.9 2022-06-14 Buy
Sigma-Aldrich(India) A9231 Acridine Orange solution 2% in H2O 65-61-2 10ML ₹13575.3 2022-06-14 Buy
Sigma-Aldrich(India) 318337 Acridine Orange hydrochloride hydrate ≥98% (HPLC) 65-61-2 1G ₹9024.3 2022-06-14 Buy
Sigma-Aldrich(India) A8097 Acridine Orange hydrochloride solution 10?mg/mL in H2O 65-61-2 10ML ₹10489.5 2022-06-14 Buy
Sigma-Aldrich(India) 318337 Acridine Orange hydrochloride hydrate ≥98% (HPLC) 65-61-2 5G ₹33300 2022-06-14 Buy
Product number Packaging Price Buy
318337 250MG ₹2985.9 Buy
A9231 10ML ₹13575.3 Buy
318337 1G ₹9024.3 Buy
A8097 10ML ₹10489.5 Buy
318337 5G ₹33300 Buy

ACRIDINE ORANGE Chemical Properties,Uses,Production

Chemical Properties

dark orange to brown powder

Uses

Acridine orange is a dye that interacts with DNA and RNA by intercelation or electrostatic attraction. This cationic dye has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels.

Preparation

4,4 ‘-Bis (N, N-dimethyl) aminodiphenylmethane obtained dinitration of 4,4′ – bis (N, N-dimethyl) amino-2 ‘- dinitro-diphenyl methane reduction, and with an acid of heat, so that the diamino compound is cyclized product is oxidized and then cyclized and converted into zinc chloride double salt.

Safety Profile

Mutation data reported. Whenheated to decomposition it emits very toxic fumes of HCl,Cl??, and NOx.

Enzyme inhibitor

This cell-permeable dye (FWfree-base = 265.36 g/mol; CAS 65-61-2), also known as N,N,N',N'-tetramethylacridine-3,6-diamine, is an intercalator that binds tightly to nucleic acids. Binding to DNA is characterized by excitation maximum at 502 nm (cyan) and an emission maximum at 525 nm, producing a green fluorescence. (A 120 μM solution of acridine orange will detect 25-50 ng DNA per band in an agarose or polyacrylamide gel.) Binding to RNA is characterized by an excitation maximum at 460 nm and the emission maximum is located at 650 nm, yielding a reddish-orange fluorescence. Acridine orange also penetrates acidic compartments (e.g., lysosomes and phagolysosomes); upon protonation, the dye emits orange light when excited by blue light.

ACRIDINE ORANGE Preparation Products And Raw materials

Global( 131)Suppliers
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Arnish Laborates Pvt Ltd +91 7208475595 Maharashtra, India 187 58 Inquiry
TCI Chemicals (India) Pvt. Ltd. 1800 425 7889 New Delhi, India 6778 58 Inquiry
CLEARSYNTH LABS LTD. +91-22-45045900 Hyderabad, India 6351 58 Inquiry
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Henan Fengda Chemical Co., Ltd +86-371-86557731 +86-13613820652 China 20293 58 Inquiry
Henan Tianfu Chemical Co.,Ltd. +86-0371-55170693 +86-19937530512 China 21667 55 Inquiry
Zhengzhou Alfa Chemical Co.,Ltd +8618530059196 China 13341 58 Inquiry
3,6-BIS(DIMETHYLAMINO)ACRIDINE HYDROCHLORIDE ACRIDINE ORANGE (E GURR) ACRIDINE ORANGE HYDROCHLORIDE ACRIDINE ORANGE N BASIC ORANGE BIS(DIMETHYLAMINO) ACRIDINE HYDROCHLORIDE AO CI NO 46005 EUCHRYSINE 3RX rhodulineorangeno N,N,N',N'-tetramethylacridin-3,6-yldiamine hydrochloride ACRIDINE ORANGE SOLUTION ACRIDINE ORANGE HYDROCHLORIDE SOLUTION, ~1% IN WATER ACRIDINE ORANGE FOR MICROSCOPY ACRIDINE ORANGE HYDROCHLORIDE HYDRATE, F OR MICROSCOPY ACRIDINE ORANGE HYDROCHLORIDE HYDRATE 80% -Cellstain-Ao -Cellstain-AoSolution ACRIDING ORANGE HYDROCHLORIDE 3,6-Bis(dimethylamino) acridine, zinc chloride double salt Acridine Orange, hydrochloride hydrate, ca. 80 % dye content, pure AOX1 acridine orange hydrochloride solution 3,6-Bis(dimethylamino)acridine,hydrochloride,hydrate,aqueoussolution ACRIDINE ORANGE N-HYRATE Basic orange 14 (C.I. 46005) Acridine Orange solution hydrochloride Acridine Orange hydrate hydrochloride Acridine Orange, hydrochloride hydrate,pure,ca. 80 % dye content Acridine Orange hydrochloride solution,3,6-Bis(dimethylamino)acridine hydrochloride ACRIDINE ORANGE HYDROCHLORIDE HYDRATE PURUM, FOR MICROSCOPY ((FL., HIST.)) 98% RHODULINE ORANGE 3,6-BIS(DIMETHYLAMINO)ACRIDINE, HCL 3,6-acridinediamine, N,n,n0,n0-tetramethyl-, monohydrochloride Acridine, 3,6-bis(dimethylamino)-, Hydrochloride Acridine, 3,6-bis(dimethylamino)-, Monohydrochloride Euchrysine 3r Sumitomo Acridine Orange No Sumitomo acridine Orange Rk Conc 3,6-Acridinediamine,N,N,N’,N’-tetramethyl-,monohydrochloride 3,6-bis(dimethylamino)-acridinmonohydrochloride 6-acridinediamine,n,n,n’,n’-tetramethyl-monohydrochloride acridineorangeno acridineorangens acridineoranger c.i.basicorange14 6-acridinediamine 6-Bis(dimethylamino)acridine hydrochloride 3 Lysotraker N’-Tetramethyl-3 N,N,N',N'-Tetramethyl-3,6-acridinediamine hydrochloride N3,N3,N6,N6-Tetramethylacridine-3,6-diamine hydrochloride Acridine Orange hydrochloride?, >99% N3,N3,N6,N6-tetramethylacridine-3,6-diamine chloride Cellstain AO Solution (1mg/ml H2O) Acridine Orange Solution [for Cell Staining] C.I.Basic Orange 14:C.I.46005 Acridine orange hydrochloride, hydrate, ≥98% (HPLC)