ChemicalBook > Product Catalog >Biochemical Engineering >Saccharides >Monosaccharide >2-Nitrophenyl-beta-D-galactopyranoside
2-Nitrophenyl-beta-D-galactopyranoside
- CAS No.
- 369-07-3
- Chemical Name:
- 2-Nitrophenyl-beta-D-galactopyranoside
- Synonyms
- ONPG;o-NPG=ONPG;2-Nitrophenyl;ONPG Solution;ONP-BETA-D-GAL;2-Nitrophenyl-beta-D;2-Nitrophenyl-b-D-galactopy;2-Nitrophenyl hexopyranoside;ONP BETA-D-GALACTOPYRANOSIDE;2-Nitrophenyl-β-D-galactoside
- CBNumber:
- CB1366392
- Molecular Formula:
- C12H15NO8
- Molecular Weight:
- 301.25
- MOL File:
- 369-07-3.mol
- MSDS File:
- SDS
- Modify Date:
- 2023/10/11 17:37:18
| Melting point | 186-193 °C (dec.) |
|---|---|
| alpha | -70 º (c=1, H2O) |
| Boiling point | 442.39°C (rough estimate) |
| Density | 1.3709 (rough estimate) |
| refractive index | -68.8 ° (C=1, H2O) |
| storage temp. | 2-8°C |
| solubility | DMSO (Slightly), Water (Slightly, Heated, Sonicated) |
| pka | 12.69±0.70(Predicted) |
| form | Powder |
| color | White to very slightly yellow |
| optical activity | [α]20/D 70±1°, c = 1% in H2O |
| Water Solubility | Soluble in water. |
| BRN | 92207 |
| InChIKey | KUWPCJHYPSUOFW-RMPHRYRLSA-N |
| CAS DataBase Reference | 369-07-3(CAS DataBase Reference) |
| NIST Chemistry Reference | Galactopyranoside, o-nitrophenyl, beta-d-(369-07-3) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H315-H319-H335 | |||||||||
| Precautionary statements | P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P362+P364-P403+P233-P501c | |||||||||
| Hazard Codes | Xi | |||||||||
| Risk Statements | 36/37/38 | |||||||||
| Safety Statements | 22-24/25-36-26 | |||||||||
| WGK Germany | 3 | |||||||||
| F | 3-10-21 | |||||||||
| HS Code | 29400090 | |||||||||
| NFPA 704 |
|
2-Nitrophenyl-beta-D-galactopyranoside price More Price(20)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich(India) | N1127 | 2-Nitrophenyl β-D-galactopyranoside ≥98% (enzymatic) | 369-07-3 | 250MG | ₹3095.95 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | N1127 | 2-Nitrophenyl β-D-galactopyranoside ≥98% (enzymatic) | 369-07-3 | 500MG | ₹4730.53 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | N1127 | 2-Nitrophenyl β-D-galactopyranoside ≥98% (enzymatic) | 369-07-3 | 1G | ₹5325.9 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | N1127 | 2-Nitrophenyl β-D-galactopyranoside ≥98% (enzymatic) | 369-07-3 | 5G | ₹15339.03 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | N1127 | 2-Nitrophenyl β-D-galactopyranoside ≥98% (enzymatic) | 369-07-3 | 25G | ₹71120.25 | 2022-06-14 | Buy |
2-Nitrophenyl-beta-D-galactopyranoside Chemical Properties,Uses,Production
Description
2-Nitrophenyl-β-D-galactopyranoside (ONPG) is a colorimetric and spectrophotometric substrate for detecting β-galactosidase activity. This compound is usually colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity utilizing a colorimetric assay (at 420 nm wavelength). β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be used to measure the enzymatic rate. Though ONPG mimics lactose and is hydrolyzed by β-galactosidase, it cannot act as an inducer for the lac operon. Without another lactose analog that can act as an inducer, such as isopropyl β-D-1-thiogalactopyranoside (IPTG), β-galactosidase will not be transcribed and ONPG will not be hydrolyzed.
Chemical Properties
White Crystalline Solid
Uses
2-Nitrophenyl-beta-D-galactopyranoside is a β-Galactosidase substrate for colorimetric and EIA applications; counterpart of widely employed pNPP/alkaline phosphatase substrate.
Definition
ChEBI: A beta-D-galactoside having a 2-nitrophenyl substituent at the anomeric position.
General Description
ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.
Purification Methods
Purify 2-nitrophenyl--D-galactopyranoside by recrystallisation from EtOH. [Seidman & Link J Am Chem Soc 72 4324 1950, Snyder & Link J Am Chem Soc 75 1758 1953]. It is a chromogenic substrate for -galactosidases [Jagota et al. J Food Sci 46 161 1981]. [Beilstein 17/7 V 52.]
2-Nitrophenyl-beta-D-galactopyranoside Preparation Products And Raw materials
Raw materials
Preparation Products
Global( 466)Suppliers
| Supplier | Tel | Country | ProdList | Advantage | Inquiry |
|---|---|---|---|---|---|
| VINEET LABORATORIES LIMITED | +91-9642200049 +91-9642200049 | Telangana, India | 69 | 58 | Inquiry |
| A.J Chemicals | 91-9810153283 | New Delhi, India | 6124 | 58 | Inquiry |
| CLEARSYNTH LABS LTD. | +91-22-45045900 | Hyderabad, India | 6351 | 58 | Inquiry |
| TCI Chemicals (India) Pvt. Ltd. | 1800 425 7889 | New Delhi, India | 6778 | 58 | Inquiry |
| Otto Chemie Pvt. Ltd. | +91 9820041841 | Mumbai, India | 5873 | 58 | Inquiry |
| Central Drug House(P) Ltd. | 91-11-49404040 | New Delhi, India | 6160 | 58 | Inquiry |
| Triveni chemicals | 08048762458 | New Delhi, India | 6093 | 58 | Inquiry |
| A. B. Enterprises | +91-8048077585 | Mumbai, India | 1396 | 58 | Inquiry |
| Evans Fine Chem | 08048602255 | Hyderabad, India | 289 | 58 | Inquiry |
| AB Enterprises | 09123436768 | Mumbai, India | 34 | 58 | Inquiry |
| Supplier | Advantage |
|---|---|
| VINEET LABORATORIES LIMITED | 58 |
| A.J Chemicals | 58 |
| CLEARSYNTH LABS LTD. | 58 |
| TCI Chemicals (India) Pvt. Ltd. | 58 |
| Otto Chemie Pvt. Ltd. | 58 |
| Central Drug House(P) Ltd. | 58 |
| Triveni chemicals | 58 |
| A. B. Enterprises | 58 |
| Evans Fine Chem | 58 |
| AB Enterprises | 58 |
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