4',6-Diamidino-2-phenylindole dihydrochloride
- CAS No.
- 28718-90-3
- Chemical Name:
- 4',6-Diamidino-2-phenylindole dihydrochloride
- Synonyms
- DAPI;DAPI.HCl;DAPI 2HCL;DAPI Stain;-Cellstain-Dapi;DAPI(meilunbio);DAPI Stain Solution;4',6-Diamidino-2-phe;DAPI (hydrochloride);DAPI, DIHYDROCHLORIDE
- CBNumber:
- CB1453377
- Molecular Formula:
- C16H16ClN5
- Molecular Weight:
- 313.79
- MOL File:
- 28718-90-3.mol
- MSDS File:
- SDS
- Modify Date:
- 2024/3/8 19:00:46
| Melting point | >300°C (dec.) |
|---|---|
| storage temp. | 2-8°C |
| solubility | H2O: 10 mg/mL heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks. |
| form | powder |
| color | Clear |
| PH | 4.0-5.0 (10g/l, H2O, 20℃) |
| λmax | 358 nm, 342 nm |
| BRN | 4894417 |
| Stability | PROTECT FROM LIGHT! Stable for 2 years as supplied. Solutions in DMSO may be aliquoted and stored at -20°C for up to 3 months. |
| Biological Applications | Nuclear apoptosis assay; targeting drug to cerebral neuron; treating amyloidosis,neurodegenerative diseases,Alzheimer’s disease,Down’s syndrome,type II diabetes; monitoring bioaerosols |
| InChIKey | FPNZBYLXNYPRLR-UHFFFAOYSA-N |
| CAS DataBase Reference | 28718-90-3(CAS DataBase Reference) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H315-H317-H335 | |||||||||
| Precautionary statements | P261-P264-P271-P272-P280-P302+P352 | |||||||||
| Hazard Codes | Xi,Xn | |||||||||
| Risk Statements | 36/37/38-20/21/22 | |||||||||
| Safety Statements | 26-36-24/25-22-37/39 | |||||||||
| WGK Germany | 3 | |||||||||
| RTECS | NL5995050 | |||||||||
| F | 3-8-10-21 | |||||||||
| HS Code | 29339900 | |||||||||
| NFPA 704 |
|
4',6-Diamidino-2-phenylindole dihydrochloride price More Price(24)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich(India) | MBD0015 | DAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1?mg/mL | 28718-90-3 | 1ML | ₹8578.5 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | MBD0015 | DAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1?mg/mL | 28718-90-3 | 5ML | ₹40365.5 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | MBD0015 | DAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1?mg/mL | 28718-90-3 | 10ML | ₹72671.2 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | MBD0020 | DAPI ready made solution with Antifade For Nuclear counterstain in Fluorescence Microscopy | 28718-90-3 | 10ML | ₹11925 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | MBD0015 | DAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1?mg/mL | 28718-90-3 | 50ML | ₹236580.4 | 2022-06-14 | Buy |
4',6-Diamidino-2-phenylindole dihydrochloride Chemical Properties,Uses,Production
Description
DAPI is a fluorescent probe which is commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It forms a fluorescent complex by attaching in the minor groove of A-
Chemical Properties
Yellow Solid
Uses
4',6-Diamidino-2-phenylindole dihydrochloride is used as a cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine.It is used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes.
General Description
DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.
References
[1] W. C. RUSSELL D. H W CAROL NEWMAN. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses[J]. Nature, 1975, 253 5491: 461-462. DOI:10.1038/253461a0.
[2] BETTY I. TARNOWSKI James H N F Spinale. DAPI as a useful stain for nuclear quantitation.[J]. Biotechnic & Histochemistry, 1991, 22 1: 297-302. DOI:10.3109/10520299109109990.
[3] ANA KATRINA ESTANDARTE. The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes.[J]. Scientific Reports, 2016: 31417. DOI:10.1038/srep31417.
4',6-Diamidino-2-phenylindole dihydrochloride Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | Inquiry |
|---|---|---|---|---|---|
| Thirumalai Chemicals Ltd | +91 (41) 7224-4441 | New Delhi, India | 3 | 0 | Inquiry |
| CHEMSWORTH | +91-261-2397244 | New Delhi, India | 6707 | 30 | Inquiry |
| TCI Chemicals (India) Pvt. Ltd. | 1800 425 7889 | New Delhi, India | 6778 | 58 | Inquiry |
| Otto Chemie Pvt. Ltd. | +91 9820041841 | Mumbai, India | 5873 | 58 | Inquiry |
| Sisco Research Laboratories Pvt. Ltd. | +91-22-4268 5800 | Mumbai, India | 4317 | 58 | Inquiry |
| Central Drug House(P) Ltd. | 91-11-49404040 | New Delhi, India | 6160 | 58 | Inquiry |
| Pharmaffiliates Analytics and Synthetics P. Ltd | +91-172-5066494 | Haryana, India | 6773 | 58 | Inquiry |
| Triveni chemicals | 08048762458 | New Delhi, India | 6093 | 58 | Inquiry |
| Uventis Bioscience | 08048372114Ext 874 | Pune, India | 5 | 58 | Inquiry |
| A.J Chemicals | 91-9810153283 | New Delhi, India | 6124 | 58 | Inquiry |
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