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4',6-Diamidino-2-phenylindole dihydrochloride

4',6-Diamidino-2-phenylindole dihydrochloride Structure
  • ₹860 - ₹236580.4
  • Product name: 4',6-Diamidino-2-phenylindole dihydrochloride
  • CAS: 28718-90-3
  • MF: C16H16ClN5
  • MW: 313.79
  • EINECS:249-186-7
  • MDL Number:MFCD00012681
  • Synonyms:2-(4-AMIDINOPHENYL)-6-INDOLECARBAMIDINEDIHYDROCHLORIDE;CELLSTAIN- DAPI SOLUTION;2-phenylindole-4',6-dicarboxamidine dihydrohydrochloride (hydrate) ;4',6-DIAMIDINO-2-PHENYLINDOLE*DIHYDROCHL ORIDE HYDRA;-Cellstain-Dapi;4',6-DIAMINO-2-PHENYLINDOLE DIHYDROCHLORIDE;DAPI, DIHYDROCHLORIDE;4',6-DIAMIDINO-2-PHENYLINDOLE, 2HCL
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  • TCI Chemicals (India)
  • Sigma-Aldrich(India)
  • SRL
  • ottokemi

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  • ManufacturerTCI Chemicals (India)
  • Product numberA2412
  • Product descriptionDAPI 2HCl [for Biochemical Research]
  • Packaging5MG
  • Price₹5500
  • Updated2022-05-26
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberD9542
  • Product descriptionDAPI for nucleic acid staining
  • Packaging1MG
  • Price₹1320.65
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberD8417
  • Product description4′,6-Diamidino-2-phenylindole dihydrochloride powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence
  • Packaging1MG
  • Price₹3182.55
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberD9542
  • Product descriptionDAPI for nucleic acid staining
  • Packaging5MG
  • Price₹6549.13
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberMBD0015
  • Product descriptionDAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1?mg/mL
  • Packaging1ML
  • Price₹8578.5
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberD8417
  • Product description4′,6-Diamidino-2-phenylindole dihydrochloride powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence
  • Packaging5MG
  • Price₹9796.63
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberD9542
  • Product descriptionDAPI for nucleic acid staining
  • Packaging10MG
  • Price₹10175.5
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product number32670
  • Product description4′,6-Diamidino-2-phenylindole dihydrochloride BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
  • Packaging25MG
  • Price₹45659.85
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberMBD0015
  • Product descriptionDAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1?mg/mL
  • Packaging10ML
  • Price₹72671.2
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberMBD0015
  • Product descriptionDAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1?mg/mL
  • Packaging50ML
  • Price₹236580.4
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product number32670
  • Product description4′,6-Diamidino-2-phenylindole dihydrochloride BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
  • Packaging5MG
  • Price₹11539.45
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberMBD0020
  • Product descriptionDAPI ready made solution with Antifade For Nuclear counterstain in Fluorescence Microscopy
  • Packaging10ML
  • Price₹11925
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberD8417
  • Product description4′,6-Diamidino-2-phenylindole dihydrochloride powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence
  • Packaging10MG
  • Price₹18608.18
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product number268298
  • Product descriptionDAPI, Dihydrochloride - CAS 28718-90-3 - Calbiochem Cell-permeable DNA-binding dye.
  • Packaging10MG
  • Price₹21510
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberMBD0015
  • Product descriptionDAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1?mg/mL
  • Packaging5ML
  • Price₹40365.5
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberD9542
  • Product descriptionDAPI for nucleic acid staining
  • Packaging50MG
  • Price₹44274.25
  • Updated2022-06-14
  • Buy
Manufacturer Product number Product description Packaging Price Updated Buy
TCI Chemicals (India) A2412 DAPI 2HCl [for Biochemical Research] 5MG ₹5500 2022-05-26 Buy
Sigma-Aldrich(India) D9542 DAPI for nucleic acid staining 1MG ₹1320.65 2022-06-14 Buy
Sigma-Aldrich(India) D8417 4′,6-Diamidino-2-phenylindole dihydrochloride powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence 1MG ₹3182.55 2022-06-14 Buy
Sigma-Aldrich(India) D9542 DAPI for nucleic acid staining 5MG ₹6549.13 2022-06-14 Buy
Sigma-Aldrich(India) MBD0015 DAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1?mg/mL 1ML ₹8578.5 2022-06-14 Buy
Sigma-Aldrich(India) D8417 4′,6-Diamidino-2-phenylindole dihydrochloride powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence 5MG ₹9796.63 2022-06-14 Buy
Sigma-Aldrich(India) D9542 DAPI for nucleic acid staining 10MG ₹10175.5 2022-06-14 Buy
Sigma-Aldrich(India) 32670 4′,6-Diamidino-2-phenylindole dihydrochloride BioReagent, suitable for fluorescence, ≥95.0% (HPLC) 25MG ₹45659.85 2022-06-14 Buy
Sigma-Aldrich(India) MBD0015 DAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1?mg/mL 10ML ₹72671.2 2022-06-14 Buy
Sigma-Aldrich(India) MBD0015 DAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1?mg/mL 50ML ₹236580.4 2022-06-14 Buy
Sigma-Aldrich(India) 32670 4′,6-Diamidino-2-phenylindole dihydrochloride BioReagent, suitable for fluorescence, ≥95.0% (HPLC) 5MG ₹11539.45 2022-06-14 Buy
Sigma-Aldrich(India) MBD0020 DAPI ready made solution with Antifade For Nuclear counterstain in Fluorescence Microscopy 10ML ₹11925 2022-06-14 Buy
Sigma-Aldrich(India) D8417 4′,6-Diamidino-2-phenylindole dihydrochloride powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence 10MG ₹18608.18 2022-06-14 Buy
Sigma-Aldrich(India) 268298 DAPI, Dihydrochloride - CAS 28718-90-3 - Calbiochem Cell-permeable DNA-binding dye. 10MG ₹21510 2022-06-14 Buy
Sigma-Aldrich(India) MBD0015 DAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1?mg/mL 5ML ₹40365.5 2022-06-14 Buy
Sigma-Aldrich(India) D9542 DAPI for nucleic acid staining 50MG ₹44274.25 2022-06-14 Buy

Properties

Melting point :>300°C (dec.)
storage temp. :2-8°C
solubility :H2O: 10 mg/mL heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.
form :powder
color :Clear
PH :4.0-5.0 (10g/l, H2O, 20℃)
λmax :358 nm, 342 nm
BRN :4894417
Stability :PROTECT FROM LIGHT! Stable for 2 years as supplied. Solutions in DMSO may be aliquoted and stored at -20°C for up to 3 months.
Biological Applications :Nuclear apoptosis assay; targeting drug to cerebral neuron; treating amyloidosis,neurodegenerative diseases,Alzheimer’s disease,Down’s syndrome,type II diabetes; monitoring bioaerosols
InChIKey :FPNZBYLXNYPRLR-UHFFFAOYSA-N
CAS DataBase Reference :28718-90-3(CAS DataBase Reference)

Safety Information

Symbol(GHS): GHS hazard pictograms
Signal word: Warning
Hazard statements:
Code Hazard statements Hazard class Category Signal word Pictogram P-Codes
H315 Causes skin irritation Skin corrosion/irritation Category 2 Warning GHS hazard pictograms P264, P280, P302+P352, P321,P332+P313, P362
H317 May cause an allergic skin reaction Sensitisation, Skin Category 1 Warning GHS hazard pictograms P261, P272, P280, P302+P352,P333+P313, P321, P363, P501
H335 May cause respiratory irritation Specific target organ toxicity, single exposure;Respiratory tract irritation Category 3 Warning GHS hazard pictograms
Precautionary statements:
P261 Avoid breathing dust/fume/gas/mist/vapours/spray.
P264 Wash hands thoroughly after handling.
P264 Wash skin thouroughly after handling.
P271 Use only outdoors or in a well-ventilated area.
P272 Contaminated work clothing should not be allowed out of the workplace.
P280 Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352 IF ON SKIN: wash with plenty of soap and water.

Description

DAPI is a fluorescent probe which is commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It forms a fluorescent complex by attaching in the minor groove of A-T rich sequences of DNA. DAPI is often used as a counterstain, as its ultraviolet (max 358 nm) excitation and blue (max 461 nm) emission wavelengths separate it nicely from many popular primary fluorophores. It can be used on either fixed or live cells, although its low permeability in live cells demands that higher concentrations be used.

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