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5,5′-Dithiobis(2-nitrobenzoic acid)

5,5′-Dithiobis(2-nitrobenzoic acid) Structure
CAS No.
69-78-3
Chemical Name:
5,5′-Dithiobis(2-nitrobenzoic acid)
Synonyms
DTNB;Ba-2767;5,5'Ellmanns;5,5&priMe;Ellmsn'sreagent;ELLMANS' REAGENT;ELLMAN'S REAGENT;ellmann's reagent;SAPONIN BIOCHEMICAL
CBNumber:
CB2215444
Molecular Formula:
C14H8N2O8S2
Molecular Weight:
396.35
MOL File:
69-78-3.mol
MSDS File:
SDS
Modify Date:
2023/12/24 21:24:35

5,5′-Dithiobis(2-nitrobenzoic acid) Properties

Melting point 240-245 °C (dec.)(lit.)
Boiling point 671.9±55.0 °C(Predicted)
Density 1.67
refractive index 1.6900 (estimate)
storage temp. Keep in dark place,Sealed in dry,Room Temperature
pka 1.57±0.25(Predicted)
form Yellow solid
color Pale Yellow to Light Yellow
Water Solubility Soluble in water and ethanol.
Merck 14,3551
BRN 1896821
Stability Stable. Incompatible with strong bases, strong oxidizing agents.
InChIKey KIUMMUBSPKGMOY-UHFFFAOYSA-N
CAS DataBase Reference 69-78-3(CAS DataBase Reference)
EPA Substance Registry System Benzoic acid, 3,3'-dithiobis[6-nitro- (69-78-3)

SAFETY

Risk and Safety Statements

Symbol(GHS) 
GHS07
Signal word  Warning
Hazard statements  H315-H319-H335
Precautionary statements  P264-P280-P302+P352+P332+P313+P362+P364-P305+P351+P338+P337+P313-P261-P280a-P304+P340-P305+P351+P338-P405-P501a
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  26-36
WGK Germany  3
RTECS  DG9650000
10
TSCA  Yes
HS Code  29309070
NFPA 704
1
2 0

5,5′-Dithiobis(2-nitrobenzoic acid) price More Price(24)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich(India) D8130 5,5′-Dithiobis(2-nitrobenzoic acid) ≥98%, BioReagent, suitable for determination of sulfhydryl groups 69-78-3 500MG ₹2200 2022-06-14 Buy
Sigma-Aldrich(India) D8130 5,5′-Dithiobis(2-nitrobenzoic acid) ≥98%, BioReagent, suitable for determination of sulfhydryl groups 69-78-3 1G ₹3970 2022-06-14 Buy
Sigma-Aldrich(India) D8130 5,5′-Dithiobis(2-nitrobenzoic acid) ≥98%, BioReagent, suitable for determination of sulfhydryl groups 69-78-3 5G ₹9660 2022-06-14 Buy
Sigma-Aldrich(India) D8130 5,5′-Dithiobis(2-nitrobenzoic acid) ≥98%, BioReagent, suitable for determination of sulfhydryl groups 69-78-3 10G ₹17820 2022-06-14 Buy
Sigma-Aldrich(India) D8130 5,5′-Dithiobis(2-nitrobenzoic acid) ≥98%, BioReagent, suitable for determination of sulfhydryl groups 69-78-3 25G ₹24258.83 2022-06-14 Buy
Product number Packaging Price Buy
D8130 500MG ₹2200 Buy
D8130 1G ₹3970 Buy
D8130 5G ₹9660 Buy
D8130 10G ₹17820 Buy
D8130 25G ₹24258.83 Buy

5,5′-Dithiobis(2-nitrobenzoic acid) Chemical Properties,Uses,Production

Chemical Properties

Crystalline

Uses

5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups.

Definition

ChEBI: An organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups.

General Description

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.

5,5′-Dithiobis(2-nitrobenzoic acid) Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 378)Suppliers
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