REVERSE TRANSCRIPTASE
- CAS No.
- 9068-38-6
- Chemical Name:
- REVERSE TRANSCRIPTASE
- Synonyms
- HIV-1 RT;abs60073;revertase;REVERSCRIPT 1;REVERSE TRANSCRIPTASE;HIV REVERSE TRANSCRIPTASE;AMV REVERSE TRANSCRIPTASE;REVERSE TRANSCRIPTASE AMV;RTL reverse transcriptase;M-MLV REVERSE TRANSCRIPTASE
- CBNumber:
- CB4702207
- Molecular Formula:
- Molecular Weight:
- 0
- MOL File:
- Mol file
- Modify Date:
- 2023/4/23 13:52:06
REVERSE TRANSCRIPTASE price More Price(1)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich(India) | M1302 | M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis | 9068-38-6 | 40000UNITS | ₹38417.1 | 2022-06-14 | Buy |
| Product number | Packaging | Price | Buy |
|---|---|---|---|
| M1302 | 40000UNITS | ₹38417.1 | Buy |
REVERSE TRANSCRIPTASE Chemical Properties,Uses,Production
Description
Enzyme possessed by retroviruses that allows transcription of DNA from an RNA template. Such an enzyme has never been isolated from other sources, but speculation about its possible occurrence continues. Such an enzyme is widely used experimentally in genetic engineering to allow the formation of complementary DNA (cDNA) from purified RNA. The discovery of this enzyme faulted the widely held central dogma, which stated that the information flow in nature was always from DNA to RNA.
Uses
Synthesizes a complementary DNA strand using single-stranded RNA or DNA in the presence of a primer
General Description
M-MLV (Moloney murine leukemia virus) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.
Purification Methods
This enzyme produces the complementary DNA from the RNA (as template). These are purified by solubilising the virus with non-ionic detergents. Lysed virions are adsorbed on DEAE-cellulose or DEAE-Sephadex columns, and the enzymes are eluted with a salt gradient, then chromatographed on a phosphocellulose column and fractions with enzyme activity are eluted in a salt gradient. They are also purified from other viral proteins by affinity chromatography on a pyran-Sepharose column. [Verna Biochim Biophys Acta 473 1 1977, Smith Methods Enzymol 65 560 1980; see commercial catalogues for other transcriptases.]
REVERSE TRANSCRIPTASE Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | Inquiry |
|---|---|---|---|---|---|
| Transiom Genomics | 08068441066Ext 367 | Gujarat, India | 10 | 58 | Inquiry |
| Shanghai Daken Advanced Materials Co.,Ltd | +86-371-66670886 | China | 18642 | 58 | Inquiry |
| career henan chemical co | +86-0371-86658258 +8613203830695 | China | 29824 | 58 | Inquiry |
| Hebei Fengjia New Material Co., Ltd | +86-0311-87836622 +86-17333973358 | China | 8057 | 58 | Inquiry |
| Nanjing Duly Biotech Co.,Ltd | +undefined18013301590 | China | 2452 | 58 | Inquiry |
| Watson Biotechnology Co.,Ltd | +86-18186686046 +86-18186686046 | China | 5852 | 58 | Inquiry |
| Sangon Biotech (Shanghai) Co., Ltd. | 800-820-1016 | CHINA | 6952 | 58 | Inquiry |
| Beijing Solarbio Science & Tecnology Co., Ltd. | 010-50973186 4009686088 | China | 18352 | 68 | Inquiry |
| Shenzhen Anran Biotechnology Co., Ltd. | 0755-26557690 | CHINA | 5 | 58 | Inquiry |
| Tianjin Cosmo Biotechnology Co., Ltd. | 13612092903 | CHINA | 34 | 58 | Inquiry |
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