SP6 RNA POLYMERASE

SP6 RNA POLYMERASE Structure
CAS No.
Chemical Name:
SP6 RNA POLYMERASE
Synonyms
SP6 RNA;SP6 RNA POLYMERASE
CBNumber:
CB4778598
Molecular Formula:
Molecular Weight:
0
MOL File:
Mol file
Modify Date:
2023/4/23 13:52:06

SP6 RNA POLYMERASE Properties

storage temp. -20°C
form solution

SP6 RNA POLYMERASE Chemical Properties,Uses,Production

Uses

SP6 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from an SP6 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:

  • RNA or DNA blotting techniques
  • In situ hybridization
  • RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
  • Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.
  • The synthesis of antisense RNA probes

General Description

With this system, homogeneously labeled single-stranded RNA can be generated. Transcripts can be non-radioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-32P] or [α-35S] labeled nucleotides.

Contents:

  • SP6 RNA Polymerase, ≥20 U/μl, in buffer, pH 7.9
  • Transcription Buffer, 10x concentrated

SP6 RNA POLYMERASE Preparation Products And Raw materials

Raw materials

Preparation Products

SP6 RNA POLYMERASE SP6 RNA