Alkaline Phosphatase
- CAS No.
- 9001-78-9
- Chemical Name:
- Alkaline Phosphatase
- Synonyms
- AP;alkaline;MCAP;CIAP;AKP;IRAP;IL1F3;HUNK1;HUNKI;Apase
- CBNumber:
- CB9690084
- Molecular Formula:
- C21H36N8O6
- Molecular Weight:
- 496.56054
- MOL File:
- 9001-78-9.mol
- MSDS File:
- SDS
- Modify Date:
- 2023/6/30 17:01:55
| Density | 1.12 g/mL at 20 °C |
|---|---|
| storage temp. | -20°C |
| form | suspension |
| color | white |
| Specific Gravity | 1 |
| Water Solubility | It is soluble in water. |
| Stability | Stability Incompatible with strong oxidizing agents |
| EPA Substance Registry System | Alkaline phosphatase (9001-78-9) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|---|---|
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P280 |
| Hazard Codes | B,Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36-24/25-22-23 |
| WGK Germany | 3 |
| F | 3-10 |
| TSCA | Yes |
| HS Code | 35079090 |
| Toxicity | An enzyme with an alkaline pH optimum that hydrolyzes phosphate monoesters. Elevation of its activity in the serum usually indicates obstructive jaundice, Paget’s disease (osteitis deformans), or bone carcinoma. |
Alkaline Phosphatase price More Price(41)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich(India) | SAE0111 | Phosphatase, Alkaline from human placenta ≥10?units/mg solid (in glycine buffer) | 9001-78-9 | 100UNITS | ₹12887.1 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | SAE0111 | Phosphatase, Alkaline from human placenta ≥10?units/mg solid (in glycine buffer) | 9001-78-9 | 1000UNITS | ₹53180.1 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | SAE0063 | Alkaline Phosphatase Recombinant solution (high-activity) | 9001-78-9 | 15MG | ₹137229.3 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | P8361 | Phosphatase, Alkaline bovine recombinant, expressed in Pichia pastoris, ≥4000?units/mg protein | 9001-78-9 | 5000UNITS | ₹45043.8 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | P7923 | Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous glycerol solution, ≥4,000?DEA units/mg protein | 9001-78-9 | 2000UNITS | ₹15462.3 | 2022-06-14 | Buy |
Alkaline Phosphatase Chemical Properties,Uses,Production
Chemical Properties
solid
Uses
Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids.
General Description
Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.
Purification Methods
The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.]
Alkaline Phosphatase Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | Inquiry |
|---|---|---|---|---|---|
| CLEARSYNTH LABS LTD. | +91-22-45045900 | Hyderabad, India | 6351 | 58 | Inquiry |
| Otto Chemie Pvt. Ltd. | +91 9820041841 | Mumbai, India | 5873 | 58 | Inquiry |
| Sisco Research Laboratories Pvt. Ltd. | +91-22-4268 5800 | Mumbai, India | 4317 | 58 | Inquiry |
| Central Drug House(P) Ltd. | 91-11-49404040 | New Delhi, India | 6160 | 58 | Inquiry |
| Triveni chemicals | 08048762458 | New Delhi, India | 6093 | 58 | Inquiry |
| Comentag Promotional Products | 08046034429 | Delhi, India | 1 | 58 | Inquiry |
| Singh Sports Wear | 07942541284 | Delhi, India | 2 | 58 | Inquiry |
| Hipax Ester Private Limited | 08048361239Ext 356 | Maharashtra, India | 4 | 58 | Inquiry |
| Bhavya Fashion | 08046048817 | Mumbai, India | 2 | 58 | Inquiry |
| Hangzhou ICH Biofarm Co., Ltd | +86-0571-28186870; +undefined8613073685410 | China | 985 | 58 | Inquiry |
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