ベンズアミジン塩酸塩N水和物 化学特性,用途語,生産方法
化学的特性
white fine crystalline powder
使用
Benzamidine hydrochloride hydrate is a reversible inhibitor of trypsin, trypsin-like enzymes, and serine proteases. It is a proteolytic inhibitor and apoptosis reagent.
主な応用
Benzamidine hydrochloride hydrate has been used:
in western blot analysis
in immunoprecipitation
in the purification of yeast dynein
as a protease inhibitor in the preparation of brush-border membrane vesicles
for the processing of human synovial fluid, blood and eye sleep
to prevent non-specific degradation of cartilages
生物活性
Peptidase inhibitor.
Benzamidine HCl is a reversible inhibitor of trypsin, trypsin-like enzymes, and serine proteases.A concentration of approximately 1 mM is used for general protease inhibition. To inhibit proteases from yeast, a range of 0.5 to 4.0 mM is used and it is for the most part interchangeable with pepstatin A.
In addition to being a strong competitive inhibitor of trypsin, benzamidine HCl has been also shown to be a strong competitive inhibitor of thrombin and plasmin. It was also found to be as effective as aprotinin in the prevention of glucagon degradation in human plasma.
純化方法
Recrystallise it from dilute HCl (crystals contain xH2O) or EtOH/few drops HCl; dry it in a vacuum. It is a proteolytic inhibitor [Jeffcoate & White J Clin Endocrinol Metab 38 155 1974, Beilstein 9 IV 898.]
予防処置
Keep container tightly sealed. Store in cool, dry conditions in well sealed containers. Incompatible with oxidizing agents. It is hygroscopic in nature.
ベンズアミジン塩酸塩N水和物 上流と下流の製品情報
原材料
準備製品