T7 RNA POLYMERASE

T7 RNA POLYMERASE C화학적 특성, 용도, 생산

용도

• T7 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from a T7 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including: RNA or DNA blotting techniques
• In situ hybridization
• RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
• Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.
• Microarray target synthesis

일반 설명

T7 RNA polymerase is commonly used to transcribe DNA which has been cloned into vectors which have two phage promoters in opposite orientation. RNA can be selectively synthesized from either strand of the insert DNA with different polymerases. Homogeneously labeled single-stranded RNA can be generated with this system. Transcripts can be non-radioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-³²P] or [α-³⁵S]-labeled nucleotides.

Synthesis of hybridization probes: T7 RNA polymerase allows highly efficient production of homogeneously labeled RNA. This labeled RNA may be used as hybridization probes in Southern, northern, and dot blots, as well as in situ hybridizations.
Suitable labels:Transcripts can be nonradioactively labeled with biotin-16-UTP or DIG-11-UTP. They may also be radioactively labeled to high specific activity with [α-32P]- or [α-35S]-labeled nucleotides.

T7 RNA POLYMERASE 준비 용품 및 원자재

원자재

준비 용품

T7 RNA POLYMERASE 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Yinjia (Shanghai) Bio-pharmaceutical Technology Co. , Ltd.	021-52992719 13512169707	product@yinjibio.com	China	29	58
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51456	80
Shanghai Kanglang Biotechnology Co., Ltd.	021-61998208 18217752821	sales@klbio.cn	China	9618	58
Absin Bioscience Inc.	021-38015121 15000105423	wulan@absin.cn	China	24731	58
Wuhan Chemstan Biotechnology Co., Ltd.	027-65317797 15926423062	422450190@qq.com	China	10436	58
Shanghai Universal Biotech Co.,Ltd	13774214275	zhouzz@univ-bio.com	China	24995	58
Jiangsu Synthgene Biotechnology Co.,Ltd.	18136497538	znf@syngenebio.com	China	901	58
Wuhan Xinzhecheng Technology Co., Ltd	17720588118	2442693357@qq.com	China	144	58
Shanghai Chemilab Biotechnology CO.,Ltd	18116465878	kmlb@kaimilaibo.com	China	2401	58
Wuhan Peptide Core Biotechnology Co., Ltd	027-65317797 13667154760	1096814016@qq.com	China	10120	58

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