T3 RNA POLYMERASE

T3 RNA POLYMERASE C화학적 특성, 용도, 생산

용도

T3 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from a T3 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:

• RNA or DNA blotting techniques
• In situ hybridization
• RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
• Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.

일반 설명

With this enzyme, homogeneously labeled single-stranded RNA can be generated. Transcripts can be nonradioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-³²P]- or [α-³⁵S]-labeled nucleotides. T3 RNA polymerase is a DNA-dependent RNA polymerase that is highly specific for its promoter. Only DNA downstream from the T3 promoter will be transcribed.

T3 RNA POLYMERASE 준비 용품 및 원자재

원자재

준비 용품

T3 RNA POLYMERASE 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80
Zhejiang Lianshuo Biotechnology Co., Ltd.	18616526224	lianshuo@vip.126.com	China	9610	58
Shanghai jingkang bioengineering co., ltd.	021-54721350 13524668266	2881505714@qq.com	China	7878	58
Shanghai Kanglang Biotechnology Co., Ltd.	021-61998208 18217752821	sales@klbio.cn	China	9618	58
Shanghai Sig Biotechnology Co., LTD	021-57810052 18930344717	2089316240@qq.com	China	9396	58
Aleshan (Guangzhou) Biotechnology Co., Ltd	18636736628	Araratbio@163.com	China	3342	58
Nantong Feiyu Biological Technology CO.,LTD	0513-68819626; 15152877458	feiyubio@163.com	China	4821	58
Shanghai Guchen Biotechnology Co., LTD	021-34675735 19147740836	1986399151@qq.com	China	9849	58
Shanghai Zheyan Biotechnology Co., LTD	18017610038 18017610038	897493074@qq.com	China	5000	58
Shanghai Ruili Spectrum Biotechnology Co., Ltd	021-64705760 18027310741	1060496435@qq.com	China	4568	58

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