스트렙톨리신O

스트렙톨리신O C화학적 특성, 용도, 생산

용도

Permeabilizes membranes to permit cellular uptake of large or charged molecules.

일반 설명

Streptolysin O (SLO) is an immunogenic, oxygen-labile toxin, hemolytic exotoxin which is reversibly activated by dithiothreitol. ^[1] It is released into the extracellular medium along with other toxins, including streptolysin S, during the growth of most strains of group A and many strains of groups C and G Streptococci. ^[1,2] SLO and Streptolysin S differ from each other in that SLO is immunogenic and oxygen-labile while Streptolysin S is oxygen-stable, nonimmunogenic and only active when associated with a carrier protein.^[3] The hemolytic activity of SLO is mediated by formation of multimeric nanopores in cholesterol containing lipid membranes.

SLO may be used for cell permeabilization or hemolysis. The susceptibility of hemolysis by SLO varies significantly for erythrocytes from different animal species.^[1] Permeabilization of cells using SLO has been performed on multiple cell types and for various applications. For instance it has been used to introduce antisense oligonucleotides into cultured eukaryotic cells;^[3] to investigate the effect of guanine nucleotide analogues on phosphatidylinositol metabolism and protein kinase C (PKC) activation in live human T lymphocytes;^[4] to monitor cholesterol oxidation within a membrane lipid bilayer; ^[5] and to label proteins inside living cells using external fluorophores.^[6]

Biochem/physiol Actions

This product is produced by recombinant expression in Escherichia coli and contains the complete native protein sequence of SLO (Uniport ID: P0DF96 aa 34-571) without any added purification tags and has calculated molecular weight of 60,144 Dalton. The material is lyophilized from a solution containing 20 mM Sodium Hepes pH 7.5, 150 mM Sodium Chloride and 2 mM EDTA.

스트렙톨리신O 준비 용품 및 원자재

원자재

준비 용품

스트렙톨리신O 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Nanjing Duly Biotech Co.,Ltd	+undefined18013301590	melody@njduly.com	China	2452	58
Creative Enzymes	1-516-855-7709	info@creative-enzymes.com	United States	8748	58
TargetMol Chemicals Inc.	+1-781-999-5354 +1-00000000000	marketing@targetmol.com	United States	19892	58
ANHUI WITOP BIOTECH CO., LTD	+8615255079626	eric@witopchemical.com	China	23555	58
Aladdin Scientific	+1-+1(833)-552-7181	sales@aladdinsci.com	United States	57511	58
Watson Biotechnology Co.,Ltd	+86-18186686046 +86-18186686046	sales01@watsonbiotech.cn	China	5852	58
Shanghai Aladdin Bio-Chem Technology Co.,LTD	400-400-6206333 18521732826	market@aladdin-e.com	China	25009	65
Nanjing Dulai Biotechnology Co., Ltd.	025-84699383-8003 18013301590	njduly@126.com	China	971	55
Guangzhou Isun Pharmaceutical Co., Ltd	020-39119399 18927568969	isunpharm@qq.com	China	4428	55
Shanghai Macklin Biochemical Co.,Ltd.	15221275939 15221275939	shenlinxing@macklin.cn	China	16168	55

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