에티듐 브로마이드
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에티듐 브로마이드 속성
- 녹는점
- 260-262 °C (dec.)(lit.)
- 밀도
- 1.3739 (rough estimate)
- 굴절률
- 1.6700 (estimate)
- 인화점
- >100°C
- 저장 조건
- 2-8°C
- 용해도
- H2O: 10 mg/mL, 불투명, 강한 빨간색
- 물리적 상태
- 가루
- 색상
- 빨간색에서 진한 보라색까지
- 냄새
- 무취 고체
- 수용성
- 40g/L(25℃)
- 최대 파장(λmax)
- 518 nm, 210 nm, 285 nm, 316 nm, 343 nm, 480 nm, 525 nm
- Merck
- 14,4731
- BRN
- 3642536
- 안정성
- 안정적인. 강한 산화제와 호환되지 않습니다.
- 생물학 분야의 응용
- Nucleic acid hybridization; detecting nucleic acids,cells,cancer cells,human cytomegalovirus,hydrogenase A (hydA) of Clostridia,influenza A virus,oligonucleotides,viable Plesiomonas shigelloides; apoptosis assay; nucleic acid quantification
- 주요 응용
- 전계발광 디스플레이, 포토레지스트
- CAS 데이터베이스
- 1239-45-8(CAS DataBase Reference)
안전
- 위험 및 안전 성명
- 위험 및 사전주의 사항 (GHS)
| 위험품 표기 | T,T+ | ||
|---|---|---|---|
| 위험 카페고리 넘버 | 23-68-36/37/38-26-21/22-22 | ||
| 안전지침서 | 36/37-45-36/37/39-28A-26-22-28-63 | ||
| 유엔번호(UN No.) | UN 2811 6.1/PG 1 | ||
| WGK 독일 | 3 | ||
| RTECS 번호 | SF7950000 | ||
| F 고인화성물질 | 8-9 | ||
| 위험 등급 | 6.1 | ||
| 포장분류 | I | ||
| HS 번호 | 29339990 | ||
| 유해 물질 데이터 | 1239-45-8(Hazardous Substances Data) | ||
| 독성 | Intercalating dye widely used to stain DNA in gels and gradients. The DNA can be visualized readily by irradiation with ultraviolet light, as little as 0.5 μg of DNA being detectable by such methods. Ethidium bromide is also added to cesium chloride density gradients, since as an intercalating molecule it binds more readily to linear DNA than to closed collinear circles of DNA (such as plasmids). Binding of ethidium bromide reduces the density of DNA, thus covalent circles of DNA have higher densities at saturating concentrations of ethidium bromide, permitting the separation of plasmid DNA. Ethidium bromide is a putative carcinogen. | ||
| 기존화학 물질 | KE-09740 |
에티듐 브로마이드 C화학적 특성, 용도, 생산
화학적 성질
Purple/maroon crystalline powder용도
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma).건강위험
Acute toxic effects from exposure to ethidium bromide have not been thoroughly investigated. Ethidium bromide is irritating to the eyes, skin, mucous membranes, and upper respiratory tract. Although there is no evidence for the carcinogenicity or teratogenicity of this substance in humans, ethidium bromide is strongly mutagenic and therefore should be regarded as a possible carcinogen and reproductive toxin인화성 및 폭발성
Ethidium bromide does not pose a flammability hazard (NFPA rating = 1).Safety Profile
Because ethidium bromide can bind with DNA, it is highly toxic as a mutagen. It may potentially cause carcinogenic or teratogenic effects, although no scientific evidence showing either health effect has been found. Exposure routes of ethidium bromide are inhalation, ingestion, and skin absorption. An acute exposure to ethidium bromide causes irritation of the mouth, upper respiratory tract, skin, and eyes.저장
Because of its mutagenicity, stock solutions of this compound should be prepared in a fume hood, and protective gloves should be worn at all times while handling this substance. Operations capable of generating ethidium bromide dust or aerosols of ethidium bromide solutions should be conducted in a fume hood to prevent exposure by inhalation.Purification Methods
Crystallise it from MeOH or EtOH [Lamos et al. J Am Chem Soc 108 4278 1986]. Its solubility in H2O is 1%. [Beilstein 22/11 V 352.] POSSIBLE CARCINOGEN.비 호환성
No incompatibilities are known.폐기물 처리
Excess ethidium bromide and waste material containing this substance should be placed in an appropriate container, clearly labeled, and handled according to your institution's waste disposal guidelines.에티듐 브로마이드 준비 용품 및 원자재
원자재
준비 용품
에티듐 브로마이드 공급 업체
글로벌( 344)공급 업체
| 공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
|---|---|---|---|---|---|
| Shaanxi Dideu Medichem Co. Ltd | +86-29-81148696 +86-15536356810 |
1022@dideu.com | China | 3882 | 58 |
| Henan Fengda Chemical Co., Ltd | +86-371-86557731 +86-13613820652 |
info@fdachem.com | China | 20288 | 58 |
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 |
info@tianfuchem.com | China | 21639 | 55 |
| career henan chemical co | +86-0371-86658258 +8613203830695 |
sales@coreychem.com | China | 29886 | 58 |
| Hubei Jusheng Technology Co.,Ltd. | 18871490254 |
linda@hubeijusheng.com | CHINA | 28172 | 58 |
| Chongqing Chemdad Co., Ltd | +86-023-6139-8061 +86-86-13650506873 |
sales@chemdad.com | China | 39894 | 58 |
| CONIER CHEM AND PHARMA LIMITED | +8618523575427 |
sales@conier.com | China | 49374 | 58 |
| Antai Fine Chemical Technology Co.,Limited | 18503026267 |
info@antaichem.com | CHINA | 9636 | 58 |
| Zhengzhou Alfa Chemical Co.,Ltd | +8618530059196 |
sale04@alfachem.cn | China | 13502 | 58 |
| TargetMol Chemicals Inc. | +1-781-999-5354 +1-00000000000 |
marketing@targetmol.com | United States | 32038 | 58 |