Triphosphopyridine nucleotide
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Triphosphopyridine nucleotide 속성
- 저장 조건
- Keep in dark place,Inert atmosphere,Store in freezer, under -20°C
- 용해도
- H2O: 50 mg/mL, 투명, 약간 노란색
- 물리적 상태
- powder to crystal
- 산도 계수 (pKa)
- pKa1 3.9; pKa2 6.1(at 25℃)
- 색상
- White to Orange to Green
- 최대 파장(λmax)
- 260nm(lit.)
- Merck
- 14,6348
- InChIKey
- XJLXINKUBYWONI-WFYQBQJJNA-N
안전
- 위험 및 안전 성명
- 위험 및 사전주의 사항 (GHS)
| 위험품 표기 | Xi | ||
|---|---|---|---|
| 위험 카페고리 넘버 | 36/37/38 | ||
| 안전지침서 | 26-36 | ||
| WGK 독일 | 3 | ||
| RTECS 번호 | UU3440000 | ||
| F 고인화성물질 | 10-21 | ||
| HS 번호 | 2934.99.9001 |
| 그림문자(GHS): |
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| 신호 어: | Warning | ||||||||||||||||||||||||||||
| 유해·위험 문구: |
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| 예방조치문구: |
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Triphosphopyridine nucleotide C화학적 특성, 용도, 생산
화학적 성질
Triphosphopyridine nucleotide is white or off-white powder, it is easy to absorb moisture and deliquescence. pKa{1}=3.9; pKa{2}=6.1. It is soluble in water, methanol, insoluble in ethanol, insoluble in ether and ethyl acetate.용도
β-Nicotinamide adenine dinucleotide phosphate hydrate is suitable for use in:the measurement of Glucose-6-phosphate dehydrogenase activity
the Cytochrome P450 3A4 assay as a part of NADPH-regenerating system
the Cytochrome P450 2D6 assay as a part of NADPH-regenerating system
the determination of Glucose-6-phosphate content
정의
The oxidized form of nicotinamide adenine dinucleotide phosphate (NADP) that receives electrons from photosystem I during photosynthesis. It exists as an anion under normal physiologic conditions.Synthesis
Triphosphopyridine nucleotide is prepared by the reaction of NADPH. It is synthesised mainly by the interaction of both NfrA1 enzyme and a Bacillus subtilis under the conditions of bacterial luciferase. Reaction conditions were as follows: with hydrogenchloride; NfrA1 enzyme; nitrofurazone; 2-amino-2-hydroxymethyl-1,3-propanediol In water at 23℃; pH=7.0; Enzyme kinetics; Further Variations; Reagents; Oxidation.
Purification Methods
Purify NMN by passage through a column of Dowex-1 (Clform) and washing with H2O until no absorbance is observed at 260 nm. The tubes containing NMN are pooled, adjusted to pH 5.5-6 and evaporated in vacuo to a small volume. This is adjusted to pH 3 with dilute HNO3 in an ice-bath and treated with 20volumes of Me2CO at 0-5o. The heavy white precipitate is collected by centrifugation at 0o. It is best stored wet and frozen or it can be dried to give a gummy residue. It has max 266nm ( 4,600) and min 249nm ( 3600) at pH 7.0 (i.e. no absorption at 340nm). It can be estimated by reaction with CNor hydrosulfite which form the 4-adducts (equivalent to NADH) which have UV max 340nm ( 6,200). Thus after reaction, an OD340 of one is obtained from a 0.1612mM solution in a 1cm path cuvette. [Plaut & Plaut Biochemical Preparations 5 56 1957, Maplan & Stolzenbach Methods Enzymol 3 899 1957, Kaplan et al. J Am Chem Soc 77 815 1955, Beilstein 22/2 V 168.]Triphosphopyridine nucleotide 준비 용품 및 원자재
원자재
앰버라이트IRC-50
트리클로르아세트 산
[(2R,5R)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxy-oxolan-2-yl]me thoxyphosphonic acid
준비 용품
Triphosphopyridine nucleotide 공급 업체
글로벌( 366)공급 업체
| 공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
|---|---|---|---|---|---|
| Maintain Biotech Co., Ltd. | +86-75589664337 +86-15112661142 |
cami@maintain-bio.com | China | 58 | 58 |
| Hubei Aumei New Material Co., Ltd. | +86-027-87366298 +8615927270571 |
tianyi@skbiology.cn | China | 282 | 58 |
| Wuhan Aoliqisi New Material Technology Co., Ltd. | +86-13545906766; +8613545906766 |
sales@whaop.com | China | 263 | 58 |
| Wuhan Golt Biotech Co., Ltd. | +8615389281203 |
maria@goltbiotech.com | China | 970 | 58 |
| SyncoZymes (Shanghai) Co., Ltd., | +86-021-68187180-811 +86-13681683526 |
lchen@syncozymes.com | China | 188 | 58 |
| Shaanxi LonierHerb Bio Technology Co Ltd | +86-86-+86-86-029-87551862 +8617702909819 |
sales006@ingredients-lonier.com | China | 2633 | 58 |
| Oxsyns Biotech | +86-86-18382622232 +8618382622232 |
yilin.fan@oxsyns.com | China | 9 | 58 |
| Wuhan Fortuna Chemical Co.,Ltd | +8618007136271 |
hk@fortunachem.com | China | 5998 | 58 |
| Hebei Weibang Biotechnology Co., Ltd | +8615531157085 |
abby@weibangbio.com | China | 8810 | 58 |
| Hebei Mojin Biotechnology Co., Ltd | +86 13288715578 +8613288715578 |
sales@hbmojin.com | China | 12839 | 58 |