灵芝酸D
| 中文名称 | 灵芝酸D |
|---|---|
| 中文同义词 | 灵芝酸D;灵芝酸D对照品,;灵芝酸D标准品 |
| 英文名称 | Ganoderic acid D |
| 英文同义词 | (7beta)-7-Hydroxy-3,11,15,23-tetraoxolanost-8-en-26-oic acid;Lanost-8-en-26-oic acid, 7-hydroxy-3,11,15,23-tetraoxo-, (7β)-;cyclophilin,colon,CypD,Ganoderic acid D,human,Apoptosis,inhibit,oxygenated,Sirtuin,tetracyclic,cancer,carcinoma,HeLa,triterpenoid,Inhibitor,deacetylated,cervical |
| CAS号 | 108340-60-9 |
| 分子式 | C30H42O7 |
| 分子量 | 514.65 |
| EINECS号 | |
| 植物来源 | 灵芝 |
| 相关类别 | 三萜;植物提取物;对照品，标准品;中药对照品;标准品;chemical reagent;pharmaceutical intermediate;phytochemical;reference standards from Chinese medicinal herbs (TCM).;standardized herbal extract;对照品;对照品-中药对照品;标准品 -中药标准品;标准品，对照品;标准品-对照品 |
| Mol文件 | 108340-60-9.mol |
| 结构式 |  |
灵芝酸D 性质
| 沸点 | 688.3±55.0 °C(Predicted) |
|---|---|
| 密度 | 1.22±0.1 g/cm3(Predicted) |
| 储存条件 | Store at -20°C, protect from light |
| 溶解度 | 溶于甲醇； |
| 形态 | 粉末 |
| 酸度系数(pKa) | 4.77±0.23(Predicted) |
| 颜色 | 白色 |
| 水溶解性 | insoluble in water |
Ganoderic acid D 是一种高度氧化的四环三萜类化合物 (tetracyclic triterpenoid)，是灵芝 (Ganoderma lucidum) 的主要活性成分。Ganoderic acid D 上调 SIRT3 的蛋白质表达并通过 SIRT3 诱导脱乙酰化的亲环蛋白 D (CypD)。Ganoderic acid D 抑制结肠癌细胞的能量重编程，包括结肠癌细胞中的葡萄糖摄取，乳酸产生，丙酮酸和乙酰辅酶的产生。Ganoderic acid D 诱导 HeLa 人宫颈癌细胞凋亡 (apoptosis)。
药理药效:抗肿瘤，保肝，心血管方面作用
|
SIRT3
|
Ganoderic acid D can inhibit the growth of numerous cancer cell lines and it inhibits HeLa human cervical carcinoma cells with an IC
50
of 17.3 mM.
Ganoderic acid D (1-50 μM; 24-72 hours) reduces the cell survival rate in a dose- and time-dependent manner.
Ganoderic acid D (10, 50 μM; 24, 48 hours) induces G2/M phase arrest.
Ganoderic acid D (10, 50 μM; 24, 48 hours) induces a morphological change typical of apoptosis in HeLa cells.
Ganoderic acid D (10 μM; 48 hours) up-regulates 14-3-3E and PRDX3.
Cell Viability Assay
| Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
| Concentration: | 1, 5, 10, 20, 50 μM |
| Incubation Time: | 24, 48, 72 hours |
| Result: | Reduced the cell survival rate in a dose- and time-dependent manner and had an IC 50 value of 17.3 μM for 48 hours treatment. |
Cell Cycle Analysis
| Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
| Concentration: | 10, 50 μM |
| Incubation Time: | 24, 48 hours |
| Result: |
Induced G2/M phase arrest.
Displayed a cell cycle profile with an elevated G2/M cell population after 24-h treatment with 10 μM. |
Apoptosis Analysis
| Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
| Concentration: | 10, 50 μM |
| Incubation Time: | 48 hours |
| Result: | Induced a morphological change typical of apoptosis in HeLa cells. |
Western Blot Analysis
| Cell Line: | HeLa human cervical carcinoma cell line (CCL-2) |
| Concentration: | 10 μM |
| Incubation Time: | 48 hours |
| Result: | Up-regulated 14-3-3E and PRDX3. |
化学性质
白色粉末，可溶于甲醇、乙醇、DMSO等有机溶剂，来源于多孔菌科灵芝Ganodermalucidum（Leyss.exFr.）Karst．的干燥子实体。用途
灵芝酸D具有降血脂、降血压的作用。用途
用于含量测定/鉴定/药理实验等药理药效:抗肿瘤，保肝，心血管方面作用