Geneticin
Geneticin атрибут
температура хранения: |
2-8°C |
растворимость: |
H2O: 50 мг/мл |
форма: |
белый порошок |
цвет: |
От белого до не совсем белого |
оптическая активность: |
[α]/D (Specific Rotation: +104.4o (c=0.3% in H2O at 26oC)) |
Растворимость в воде: |
растворимый |
Стабильность:: |
Hygroscopic |
ИнЧИКей: |
QNQZPJLBGRQFDD-ZLRXUJDMSA-N |
FDA UNII: |
A08F5XTI6G |
безопасность
- Заявления о рисках и безопасности
- код информации об опасности(GHS)
символ(GHS) |
|
сигнальное слово |
Danger |
Заявление об опасности |
пароль |
Заявление об опасности |
Класс опасности |
категория |
сигнальное слово |
пиктограмма |
предупреждение |
H317 |
При контакте с кожей может вызывать аллергическую
реакцию. |
Сенсибилизация, Кожа |
Категория 1 |
Предупреждение |
![](/GHS07.jpg) |
P261, P272, P280, P302+P352,P333+P313, P321, P363, P501 |
H334 |
При вдыхании может вызывать аллергическую реакцию
(астму или затрудненное дыхание). |
Сенсибилизация, респираторный |
Категория 1 |
Опасность |
![](/GHS08.jpg) |
P261, P285, P304+P341, P342+P311,P501 |
|
Внимание |
P261 |
Избегать вдыхания пыли/ дыма/ газа/ тумана/ паров/
аэрозолей. |
P272 |
Не уносить загрязненную спецодежду с места работы. |
P280 |
Использовать перчатки/ средства защиты глаз/ лица. |
P284 |
Использовать средства защиты органовдыхания. |
P302+P352 |
ПРИ ПОПАДАНИИ НА КОЖУ: Промыть большим количеством
воды. |
P333+P313 |
При возникновении раздражения или покраснения кожи
обратиться за медицинской помощью. |
|
Geneticin химические свойства, назначение, производство
Описание
Geneticin (also known as G418, G418 sulfate) is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. Geneticin blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells and is commonly used in laboratory research to select genetically engineered cells. Resistance to Geneticin is conferred by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APH 3'II.
Химические свойства
white to off-white powder
Использование
Geneticin is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL. It is also used to membrane-permeant allosteric effector of hemoglobin therapeutic in cardiovascular diseases and cancer.
прикладной
Geneticin's most common application is in molecular biology as a selection agent. Geneticin(G418 sulfate) is toxic to bacteria, yeast, protozoa, helminths, and mammalian cells. Resistance is conferred by one of two dominant genes of bacterial origin which can be expressed in eukaryotic cells. G418 is water soluble and can be stored at room temperature for as long as 1 year. Aqueous solutions should be stored frozen. The amount of G418 required for selection will vary with each cell type and growth cycle. Although cells that are multiplying will be affected sooner than those that are not, cells that are in log phase will still require 3 to 7 days for selection. In general, the amount of G418 required for selection of mammalian cells is 400 mg/ml and 200 mg/ml for maintenance.
Общее описание
The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 μg/ml Plant cells: 10-100 μg/ml Yeast cells: 0.5-1.0 μg/ml Mammalian cells: 0.1-2.0 μg/ml
Способ действия
Geneticin works by blocking polypeptide synthesis by preventing the elongation step within eukaryotic cells.
Resistance: Resistance to Geneticin is conferred through the NPTll gene in plant cells and the NEO gene in mammalian cells, which encodes aminoglycoside 3'-phosphotransferase.
Action: Geneticin functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation.
Geneticin препаратная продукция и сырье
сырьё
препарат
Geneticin поставщик
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