![](/CAS/GIF/3326-34-9.gif)
5-Aminofluorescein synthesis
- Product Name:5-Aminofluorescein
- CAS Number:3326-34-9
- Molecular formula:C20H13NO5
- Molecular Weight:347.32
![4-Aminophthalic acid](/CAS/GIF/5434-21-9.gif)
5434-21-9
251 suppliers
$6.00/1g
![Resorcinol](/CAS/GIF/108-46-3.gif)
108-46-3
747 suppliers
$10.00/10g
![5-Aminofluorescein](/CAS/GIF/3326-34-9.gif)
3326-34-9
192 suppliers
$15.00/250mg
Yield:3326-34-9 94.5%
Reaction Conditions:
with Eaton′s Reagent at 97; for 3 h;Inert atmosphere;Temperature;
Steps:
1.2; 2.2; 3.2 Preparation of 5-aminofluorescein:
add 1.1 g (6.0 mmol) to the three-necked flask4-aminophthalic acid prepared in step (1), and then added 1.39 g in turn(12.6 mmol) resorcinol, 10 mL methanesulfonic acid, under argon,The reaction was carried out at 97 °C for 3 h, cooled to room temperature, and the resulting reaction solution was poured into 30 mL of ice water.The pH of the system was adjusted to 6 with 5 mol/L NaOH solution, and a reddish-brown solid was precipitated.After refrigerating at 5°C for 2 hours, no solid was precipitated, suction filtered, and the filter cake was washed with water until neutral.Vacuum dried to constant weight at 50°C to obtain 2.03 g of a tan solid,It is 5-aminofluorescein with a yield of 94.5%.
References:
CN115160283,2022,A Location in patent:Paragraph 0018-0023
![4-Nitrofluorescein](/CAS/GIF/3326-35-0.gif)
3326-35-0
91 suppliers
$59.00/250mg
![5-Aminofluorescein](/CAS/GIF/3326-34-9.gif)
3326-34-9
192 suppliers
$15.00/250mg
![4-Nitrophthalic acid](/CAS/GIF/610-27-5.gif)
610-27-5
412 suppliers
$6.00/5g
![5-Aminofluorescein](/CAS/GIF/3326-34-9.gif)
3326-34-9
192 suppliers
$15.00/250mg
![5-Nitrofluorescein diacetate](/CAS/20180808/GIF/14926-29-5.gif)
14926-29-5
30 suppliers
inquiry
![5-Aminofluorescein](/CAS/GIF/3326-34-9.gif)
3326-34-9
192 suppliers
$15.00/250mg
![4-Nitrophthalic anhydride](/CAS/GIF/5466-84-2.gif)
5466-84-2
230 suppliers
$11.00/1g
![5-Aminofluorescein](/CAS/GIF/3326-34-9.gif)
3326-34-9
192 suppliers
$15.00/250mg