ChemicalBook CAS DataBase List Cytidine 5’-monophosphate

Cytidine 5’-monophosphate synthesis

6synthesis methods

Product Name:Cytidine 5’-monophosphate
CAS Number:63-37-6
Molecular formula:C9H14N3O8P
Molecular Weight:323.2

As a key intermediate in preparing several nucleotide derivatives, cytidine 5′-monophosphate (5′-CMP) is widely applied in food and pharmaceutical industries. Enzymatic synthesis of 5′-CMP is catalyzed by cytidine kinase (CK, EC 2.7.1.213), a type of nucleoside kinase that belongs to the PfkB family. Li et al. constructed a highly efficient biosynthesis system for manufacturing 5′-CMP in vitro. CK was used for the biotransformation of cytidine to 5′-CMP, while polyphosphate kinase (PPK) was coupled for adenosine triphosphate regeneration. Both CK and PPK were selected from extremophiles, possessing great potential for biocatalytic synthesis. After optimization, 96 mM 5′-CMP was produced within 6 h, and the yield reached nearly 100%[1].

3616-08-8 Synthesis

3616-08-8
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63-37-6
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Yield:-

Reaction Conditions:

with bovine serum albumin in aq. buffer at 30;Enzymatic reaction;

Steps:

2.3. Time course of PDE7A activity

General procedure: Time course experiments were performed in PDE buffer + 0.05% (m/v) BSA at a cAMP- or cCMP-concentration of 3 μM. The purified GST-tagged and truncated PDE7A1/2 was added at a concentration of 0.95 μg/ml, corresponding to 210 pmol/(min ml). Crude PDE7A1-containing Sf9 cell lysate was added to yield a final activity of 5 U/ml. The samples were incubated at 30 °C under constant shaking. Aliquots were drawn at appropriate times, processed as described for the enzyme screening experiments (Section 2.2) and analyzed by HPLC-MS/MS as described in the Supplementary Methods. In all experiments, the samples were repeatedly subjected to short centrifugations (at least every 30 min) to avoid concentration changes by evaporation- and condensation processes.

References:

Monzel, Maike;Kuhn, Maike;B?hre, Heike;Seifert, Roland;Schneider, Erich H. [FEBS Letters,2014,vol. 588,# 18,p. 3469 - 3474]

362-42-5
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63-37-6
579 suppliers
$12.00/25g

References

65-46-3 Synthesis

65-46-3
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63-37-6
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$12.00/25g

References

5'-Cytidylic acid, 2',3'-O-(1-methylethylidene)-

149759-93-3
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63-37-6
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References

5'-Cytidylic acid, 2',3'-bis-O-[(1,1-dimethylethyl)dimethylsilyl]-, bis(2,2,2-trichloroethyl) ester (9CI)

78571-59-2
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63-37-6
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References

Cytidine 5’-monophosphate Related Search:

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