Uridine synthesis
- Product Name:Uridine
- CAS Number:58-96-8
- Molecular formula:C9H12N2O6
- Molecular Weight:244.2
65-46-3
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58-96-8
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Yield:58-96-8 99%
Reaction Conditions:
with cytidine deaminase enzyme in aq. phosphate buffer at 37; pH=7; for 0.0833333 h;Enzymatic reaction;
Steps:
1 Comparative Example 1 : Deamination of cytidine (compound 1 ) to uridine
Comparative Example 1 : Deamination of cytidine (compound 1 ) to uridine A 100 mM solution of the cytidine (495 μΙ) in 100 mM phosphate buffer at pH 7 was mixed with 50 L of cytidine deaminase enzyme solution containing >300 AU in phosphate buffer. The reaction was performed at 37°C during 5 minutes and stopped with HCI. Then, the crude reaction was filtered through a 10 KDa membrane, and a portion was diluted and analyzed by HPLC under UV-DAD (ultraviolet-diode array detection). Product identification was performed by comparison to a standard sample. Uridine was obtained in quantitative yield (>99%).
References:
WO2016/146808,2016,A1 Location in patent:Page/Page column 28; 29
1748-04-5
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6554-10-5
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![Uridine, 2',3',5'-tris-O-[(1,1-dimethylethyl)dimethylsilyl]-](/CAS/20210305/GIF/64898-15-3.gif)
64898-15-3
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58-96-8
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362-43-6
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58-96-8
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