5(6)-Aminofluorescein

5(6)-Aminofluorescein Structure
CAS No.
27599-63-9
Chemical Name:
5(6)-Aminofluorescein
Synonyms
5(6)-AFM;FLUORESCEINAMINE;AMINOFLUORESCEIN;4-AMINOFLUORESCEIN;1-AMINOFLUORESCEIN;1-FLUORESCEINAMINE;5(6)-AMinofluorescei;5(6)-Aminofluorescein;Metrifonate Impurity 3;FLUORESCEINAMINE ISOMER I
CBNumber:
CB0254490
Molecular Formula:
C20H13NO5
Molecular Weight:
347.32
MOL File:
27599-63-9.mol
Modify Date:
2024/3/28 17:11:29

5(6)-Aminofluorescein Properties

Melting point 223 °C (dec.)(lit.)
Density 1.64
storage temp. 2-8°C
solubility DMSO (Slightly, Heated), Methanol (Very Slightly)
form powder
color red-brown
Stability Light and Moisture Sensitive

SAFETY

Risk and Safety Statements

Symbol(GHS) 
GHS07
Signal word  Warning
Hazard statements  H302-H315-H319-H335
Precautionary statements  P261-P280-P301+P312-P302+P352-P305+P351+P338
Hazard Codes  Xn
Risk Statements  22-36/37/38
Safety Statements  26
WGK Germany  3
10
HS Code  29321900

5(6)-Aminofluorescein Chemical Properties,Uses,Production

Uses

Fluoresceinamine is a fluorescent labeling precursor used to prepare FITC.

Purification Methods

Dissolve it in EtOH, treat with charcoal, filter, evaporate and dry the residue in a vacuum at 100o overnight. Also recrystallise it from 6% HCl, then dissolve it in 0.5% aqueous NaOH and precipitate it by acidifying with acetic acid. The separate amines are made from the respective nitro compounds, which are best separated via their acetate salts. They have similar RF of 0.26 on Silica Gel Merck F254 in 5 mL MeOH + 150 mL Et2O saturated with H2O. IR (Me2SO) has a band at max 1690 cm-1 (CO2-) and sometimes a weak band at 1750 cm-1 due to the lactone. UV (EtOH) of the 6-max isomer has max at 222nm ( 60 000) and the 5-isomer at max 222nm ( 60 000) and 285nm ( 20.600). [IR: McKinney & Churchill J Chem Soc (C) 654 1970, McKinney et al. J Org Chem 27 3986 1962, UV: Verbiscar J Org Chem 29 490 1964, Beilstein 19 III/IV 4337, 19/8 V 713.]

5(6)-Aminofluorescein Preparation Products And Raw materials

Raw materials

Preparation Products

5(6)-Aminofluorescein Suppliers

Global( 107)Suppliers
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FLUORESCEINAMINE FLUORESCEINAMINE, ISOMER 1 FLUORESCEINAMINE ISOMER I 4-AMINOFLUORESCEIN ISOMER I 4-AMINOFLUORESCEIN 5-AMINO-3',6'-DIHYDROXYSPIRO[ISOBENZOFURAN-1(3H),9'-(9H)XANTHEN]-3-ONE 5-AMINOFLUORESCEIN ISOMER I 5(6)-Aminofluorescein 1-FLUORESCEINAMINE 1-AMINOFLUORESCEIN fluoresceinamine, mixture of isomers FLUORESCEINAMINE, FOR FLUORESCENCE, MIXT URE OF ISOMERS 5(6)-AMinofluorescei 5(6)-AFM AMINOFLUORESCEIN 5(6)-Aminofluorescein, mixture of isomer 5(or 6)-Amino-3′,6′-dihydroxyspiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5(or 6)-amino-3',6'-dihydroxy- Metrifonate Impurity 3 27599-63-9 Fluorescent Probes, Labels, Particles and Stains Fluorescent Labels Fluorescein and Derivatives Detection Biochemicals and Reagents BioChemical