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5-Aminofluorescein

5-Aminofluorescein Structure
CAS No.
3326-34-9
Chemical Name:
5-Aminofluorescein
Synonyms
5-AF;FLUORESCEINAMINE;5-AFM;AMINOFLUORESCEIN;4-AMINOFLUORESCEIN;(isoMer I);5-AMINOFLUORESCEIN;1-FLUORESCEINAMINE;1-AMINOFLUORESCEIN;Fluoresceinamine I
CBNumber:
CB3254491
Molecular Formula:
C20H13NO5
Molecular Weight:
347.32
MOL File:
3326-34-9.mol
Modify Date:
2024/7/11 17:04:31

5-Aminofluorescein Properties

Melting point 223 °C (dec.)(lit.)
Boiling point 481.89°C (rough estimate)
Density 1.3724 (rough estimate)
refractive index 1.5180 (estimate)
storage temp. room temp
solubility DMF: 5 mg/ml; DMSO: 5 mg/ml
pka 9.34±0.20(Predicted)
form powder
color red-brown
Water Solubility Soluble in water (partly), methanol (10 mg/ml), and acetone.
BRN 48395
Stability Light and Moisture Sensitive
InChIKey GZAJOEGTZDUSKS-UHFFFAOYSA-N
CAS DataBase Reference 3326-34-9(CAS DataBase Reference)
EPA Substance Registry System Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5-amino-3',6'-dihydroxy- (3326-34-9)

SAFETY

Risk and Safety Statements

Symbol(GHS) 
GHS07
Signal word  Warning
Hazard statements  H302-H315-H319-H335
Precautionary statements  P261-P264-P270-P301+P312-P302+P352-P305+P351+P338
Hazard Codes  Xn
Risk Statements  22-36/37/38
Safety Statements  26
WGK Germany  3
10
TSCA  Yes
HS Code  32049090
NFPA 704
0
2 0

5-Aminofluorescein price More Price(5)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich(India) 201626 Fluoresceinamine, isomer I 3326-34-9 250MG ₹4417.8 2022-06-14 Buy
Sigma-Aldrich(India) 201626 Fluoresceinamine, isomer I 3326-34-9 1G ₹14352.3 2022-06-14 Buy
Sigma-Aldrich(India) 201626 Fluoresceinamine, isomer I 3326-34-9 5G ₹50349.6 2022-06-14 Buy
TCI Chemicals (India) A0306 5-Aminofluorescein (isomer I) 3326-34-9 1G ₹9900 2022-05-26 Buy
TCI Chemicals (India) A0306 5-Aminofluorescein (isomer I) 3326-34-9 5G ₹35300 2022-05-26 Buy
Product number Packaging Price Buy
201626 250MG ₹4417.8 Buy
201626 1G ₹14352.3 Buy
201626 5G ₹50349.6 Buy
A0306 1G ₹9900 Buy
A0306 5G ₹35300 Buy

5-Aminofluorescein Chemical Properties,Uses,Production

Chemical Properties

Dark Red Solid

Uses

Glycosaminoglycuronans react with 5-aminofluorescein to yield fluorescent derivatives. 5-aminofluorescein is also used to prepare FITC Isomer I (F-011, F-020).

Definition

ChEBI: 5-aminofluorescein is a primary amino compound that is fluorescein carrying an amino substituent at C-5. Building block/intermediate for the synthesis of the fluorescent dye flourescein; also used to produce N-(fluorescein-5-yl)maleamic acid. It derives from a fluorescein (lactone form).

Preparation

The preparation of 5-aminofluorescein is carried out either by first saponification of 5-nitrofluorescein acetates with alcohol alkali, followed by reduction of the obtained 5-nitrofluorescein in a solution of alcohol alkali with hydrazine hydrate on a nickel catalyst to obtain 5-aminofluorescein , Or by boiling in an aqueous solution of sodium sulfide. The restoration of the nitro group and the saponification of acetates in the second case occurs in one stage.

General Description

Fluoresceinamine, isomer I belongs to the class of derivatized fluoresceins.

5-Aminofluorescein Preparation Products And Raw materials

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5-Amino-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-(9H)xanthen]-3-one Fluoresceinamine 5-amino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one FLUORESCEINAMINE FLUORESCEINAMINE ISOMER I FLUORESCEINAMINE, ISOMER 1 5-amino-2-(3-hydroxy-6-oxoxanthen-9-yl)benzoic acid 5-AMINO-3',6'-DIHYDROXYSPIRO[ISOBENZOFURAN-1(3H),9'-(9H)XANTHEN]-3-ONE 5-AMINOFLUORESCEIN ISOMER I 5-AMINOFLUORESCEIN 4-AMINOFLUORESCEIN ISOMER I 4-AMINOFLUORESCEIN 5-AFM AMINOFLUORESCEIN 1-FLUORESCEINAMINE 1-AMINOFLUORESCEIN 9’-[9h]xanthen]-3-one,5-amino-3’,6’-dihydroxy-spiro[isobenzofuran-1(3h Fluoresceinamine,isomerⅡ 5-AMINOFLUORESCEIN, FOR FLUORESCENCE FLUORESCEIN AMINE ISOMER I 99% 4-AMINOFLUORESCEIN (ISOMER 1) Fluoresceinamine isomer I, pure FLUORESCENAMINEISOMERI 6-Amino-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one Fluoresceinamine I Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5-amino-3',6'-dihydroxy- 3,6-Dihydroxy-5'-aminospiro[9H-xanthene-9,1'(3'H)-isobenzofuran]-3'-one (isoMer I) 5-AMinofluorescein  FluoresceinaMine isoMer I, pure 1GR FluoresceinaMine isoMer I, pure 5GR Fluoresceinamine, isomer I,5-Aminofluorescein 5-Fluoresceinamine isomer I 5-Aminofluorescein (isomer I)> 5-AF 5-Aminofluorescein (5-AF) 5-amino-3',6'-dihydroxy-3H-spiro[2-benzofuran-1, 9'-xanthen]-3-one *Desflurane Impurity 6 (Desflurane EP Impurity F) 5-Amino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one 3326-34-9 C20H13O5 C20H13NO5 Biochemicals and Reagents BioChemical Detection Fluorescein and Derivatives Fluorescent Labels Fluorescent Probes, Labels, Particles and Stains marker Fluorescent Labels and Indicators Fluorescent Labels & Indicators