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ChemicalBook > Product Catalog >API >Fluid, Electrolyte, and Acid-Base Balance >Acid-base balance regulating drugs >Tris(hydroxymethyl)aminomethane

Tris(hydroxymethyl)aminomethane

Tris(hydroxymethyl)aminomethane Structure
CAS No.
77-86-1
Chemical Name:
Tris(hydroxymethyl)aminomethane
Synonyms
TRIS;TROMETHAMINE;TROMETAMOL;TRIS(HYDROXYMETHYL)AMINOMETHANE;2-AMINO-2-(HYDROXYMETHYL)PROPANE-1,3-DIOL;TRIS BUFFER;TRIZMA BASE;Trizma;TRIS AMINO;THAM
CBNumber:
CB8336488
Molecular Formula:
C4H11NO3
Molecular Weight:
121.14
MOL File:
77-86-1.mol
MSDS File:
SDS
Modify Date:
2024/6/27 18:15:55
Request For Quotation

Tris(hydroxymethyl)aminomethane Properties

Melting point 167-172 °C(lit.)
Boiling point 219-220 °C10 mm Hg(lit.)
Density 1,353 g/cm3
vapor pressure 0.0267 hPa (20 °C)
refractive index 1.4170 (estimate)
Flash point 219-220°C/10mm
storage temp. 20-25°C
solubility H2O: 4 M at 20 °C, clear, colorless
form crystalline
color white
PH Range 7 - 9
PH 10.5-12.0(4 m in water, 25 °C)
pka 8.1(at 25℃)
Water Solubility 550 g/L (25 ºC)
λmax λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.08
Sensitive Hygroscopic
Merck 14,9772
BRN 741883
Stability Stable. Incompatible with bases, strong oxidizing agents. Protect from moisture.
InChIKey LENZDBCJOHFCAS-UHFFFAOYSA-N
LogP -2.515 (est)
CAS DataBase Reference 77-86-1(CAS DataBase Reference)
NIST Chemistry Reference 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-(77-86-1)
EPA Substance Registry System Tris(hydroxymethyl)aminomethane (77-86-1)

SAFETY

Risk and Safety Statements

Symbol(GHS) 
GHS07
Signal word  Warning
Hazard statements  H315-H319-H335
Precautionary statements  P261-P264-P271-P280-P302+P352-P305+P351+P338
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  26-36-37/39-24/25-23
WGK Germany  2
RTECS  TY2900000
F  3
TSCA  Yes
HazardClass  IRRITANT
HS Code  29221980
Toxicity LD50 orally in Rabbit: > 5000 mg/kg LD50 dermal Rat > 5000 mg/kg
NFPA 704
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Tris(hydroxymethyl)aminomethane price More Price(83)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich(India) TRIS-RO Tris base =99.9% (titration), abs., 100 mg/mL, 300?nm ≤0.015 77-86-1 1KG ₹19441.7 2022-06-14 Buy
Sigma-Aldrich(India) T6791 Trizma® base BioXtra, pH 10.5-12.0 (1?M in H2O), ≥99.9% (titration) 77-86-1 100G ₹10056.43 2022-06-14 Buy
Sigma-Aldrich(India) TRIS-RO Tris base =99.9% (titration), abs., 100 mg/mL, 300?nm ≤0.015 77-86-1 5KG ₹51440.4 2022-06-14 Buy
Sigma-Aldrich(India) T6791 Trizma® base BioXtra, pH 10.5-12.0 (1?M in H2O), ≥99.9% (titration) 77-86-1 500G ₹23468.6 2022-06-14 Buy
Sigma-Aldrich(India) T6687 Tromethamine meets USP testing specifications 77-86-1 100G ₹4490 2022-06-14 Buy
Product number Packaging Price Buy
TRIS-RO 1KG ₹19441.7 Buy
T6791 100G ₹10056.43 Buy
TRIS-RO 5KG ₹51440.4 Buy
T6791 500G ₹23468.6 Buy
T6687 100G ₹4490 Buy

Tris(hydroxymethyl)aminomethane Chemical Properties,Uses,Production

Chemical Properties

White, crystalline solid. PH (0.1M aqueous solution) 10.6, corrosive to copper, brass, aluminum. Solubility in water 80 g/100 cc (20C). Combustible.

Uses

Tris base has been used:

  • In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
  • As a component of sample buffer for SDS-PAGE
  • In the preparation of IEC (ion-exchange chromatography) buffer

Uses

Tris(hydroxymethyl)aminomethane could used in the synthesis of surface-active agents, vulcanization accelerators, pharmaceuticals. As emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, leather dressings, textile specialties, polishes, cleaning Compounds, so-called soluble oils. Absorbent for acidic gases. Biological buffer.

Application

Tris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.
Tris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.
Tris(hydroxymethyl)aminomethane is used as a buffer in biochemistry and molecular biology laboratories. It is used as a primary standard to standardize acid solutions for chemical analysis. It finds application in cell membranes to increase permeability. As an alternative to sodium bicarbonate, it used in the treatment of metabolic acidosis. It acts as a precursor for the preparation of polymers, oxazolones and oxazolidines.

Preparation

Tris(hydroxymethyl)aminomethane is prepared industrially by the exhaustive condensation of nitromethane with formaldehyde under basic conditions (i.e. repeated Henry reactions) to produce the intermediate (HOCH2)3CNO2, which is subsequently hydrogenated to give the final product.

General Description

Tris(hydroxymethyl)aminomethane is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Biochem/physiol Actions

Trizma base, commonly known as tris(hydroxymethyl)aminomethane is widely used as a biological buffer or as a component of buffer formulations like- Tris-EDTA (TE) buffer, Tris magnesium buffer or Tris-acetate-EDTA (TAE) buffer. It enhances the membrane permeability of cell membranes.

Purification Methods

Tris(hydroxymethyl)aminomethane can ordinarily be obtained in highly pure form suitable for use as an acidimetric standard. If only impure material is available, it should be crystallised from 20% EtOH, aqueous MeOH (m 171.1o) or isopropanol (m 172-173o). Dry it in a vacuum desiccator over P2O5 or CaCl2. Alternatively, it is dissolved in twice its weight of water at 55-60o, filtered, concentrated to half its volume and poured slowly, with stirring, into about twice its volume of EtOH. The crystals which separate on cooling to 3-4o are filtered off, washed with a little MeOH, air dried by suction, then finally ground and dried in a vacuum desiccator over P2O5. It has also been recrystallised from water, MeOH or aqueous MeOH, and vacuum dried at 80o for 2 days. [Beilstein 4 H 303, 4 III 857, 4 IV 1903.]

Tris(hydroxymethyl)aminomethane Preparation Products And Raw materials

Raw materials

Nitromethane Potassium carbonate Paraformaldehyde Palladium Methanol
Tris(hydroxymethyl)nitromethane Hydrogen Formaldehyde
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Preparation Products

Tricine N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid YM 273 TRIS hydrochloride

Tris(hydroxymethyl)aminomethane Suppliers

Global( 1105)Suppliers
Supplier Tel Country ProdList Advantage Inquiry
GLR Innovations +91 9891111994 New Delhi, India 4542 58 Inquiry
ORGANO FINE CHEMICALS +91-91-22-42128300 +91-2242128300 Mumbai, India 258 58 Inquiry
R SCIENTIFIC +919000280374 Hyderabad, India 143 58 Inquiry
JSK Chemicals +919879767970 Gujarat, India 3756 58 Inquiry
Otto Chemie Pvt Ltd +91-2222070099 +91-2266382599 Maharashtra, India 429 58 Inquiry
CHEMAZON LABORATORIES +91 9848551964 Hyderabad, India 1320 58 Inquiry
RVR Labs Pvt Ltd +91-9912882943 +91-9912882943 Hyderabad, India 53 58 Inquiry
Oxford laboratories +91 250 239 0032 Maharashtra, India 652 58 Inquiry
Titan Biotech Limited 09711169006 Delhi, India 196 58 Inquiry
Opulent Pharma 08068441147Ext 981 Gujarat, India 810 58 Inquiry
Supplier Advantage
GLR Innovations 58
ORGANO FINE CHEMICALS 58
R SCIENTIFIC 58
JSK Chemicals 58
Otto Chemie Pvt Ltd 58
CHEMAZON LABORATORIES 58
RVR Labs Pvt Ltd 58
Oxford laboratories 58
Titan Biotech Limited 58
Opulent Pharma 58

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Tris(hydroxymethyl)aminomethane Spectrum

Tris(hydroxymethyl)aminomethane(77-86-1)MSTris(hydroxymethyl)aminomethane(77-86-1)1HNMRTris(hydroxymethyl)aminomethane(77-86-1)13CNMRTris(hydroxymethyl)aminomethane(77-86-1)IR1Tris(hydroxymethyl)aminomethane(77-86-1)IR2Tris(hydroxymethyl)aminomethane(77-86-1)Raman

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Tromethamine, USP Grade Tris, USP Grade Tris(hydroxymethyl)aminomethane (TRIS)99.9% Tris-hydroxymethylaminomethane: (THAM) TRIS(HYDROXYMETHYL) AMINOMETHANE pure AR TRIS(HYDROXYMETHYL)AMINOMETHANE extrapure AR TRIS (HYDROXYMETHYL) AMINO METHANE USP TRIS(HYDROXYMETHYL)AMINOMETHANE, 99.8+%, A.C.S. REAGENT TRIZMA(R) BASE, BIOTECHNOLOGY PERFORMANCE CERTIFIED, CELL CULTURE TESTED MEETS EP & USP TESTING SPECIFICATIONS TRIS(HYDROXYMETHYL)AMINOMETHANE, 99+% TRIZMA BASE TRIZMA BASE ELECTROPHORESIS REAGENT TRIZMA(R) PRE-SET CRYSTALS, TYPE 7.4-FT, FISH GRADE, PH 7.4 TRIZMA BASETHYL), FOR MOLECULAR BIOLOGY* TRIZMA BASE REAGENT GRADE TRIS(HYDROXYMETHYL)AMINOMETHANE 99.8+%& TRIZMA TYPE 8.3 FT TRIZMA BASE SOLUTION TRIZMA BASE, FOR LUMINESCENCE* TRIZMA(R) BASE, 4X10 G* TRIZMA(R) BASE, PH EUR TRIZMA BASE SOLUTION, 1.5M TRIZMA BASE BUFFER TROMETHAMINE USP TRIZMA BASE FOR BIOTECHNOLOGY,*DIAGNOSTI CS OR, TRIS, certified standard titrimetric substance TRIS(HYDROXYMETHYL)AMINOMETHANE, 99.9+%, ULTRAPURE GRADE TRIZMA BASE SIGMAULTRA TRIS ACIDIMETRIC NIST STANDARD TRIS TRIS(HYDROXYMETHYL)AMINOMETHANE 99% TRIS BUFFER 1.0 M SOLUTION, PH 7.5 TRIS TRIS(HYDROXYMETHYL)AMINOMETHANE BUFFER SUBSTANCE GR ACS 99.5+% TRIS BUFFER 1.0 M SOLUTION, PH 9.0 TRIS TRIS(HYDROXYMETHYL)AMINOMETHANE BIOTECHNOLOGY GRADE 99.9+% TRIS BUFFER 0.1 M SOLUTION, PH 7.4 TRIS BUFFER 2.0 M SOLUTION, PH 7.8 TRIS BUFFER 0.5 M SOLUTION, PH 6.8 TRIS TRIS(HYDROXYMETHYL)AMINOMETHANE MOLECULAR BIOLOGY REAGENT 99.9+% TRIS BUFFER 1.0 M SOLUTION, PH 10.0 TRIS BUFFER (1 M) PH 8.0 TRIS BUFFER (1 M) PH 7.4 TRIS BUFFER USP 25 (TROMETHAMINE) TRIS - SOLUTION FOR PHENOL EQUILIBRATION TRIS ULTRAPURE (TRIS(HYDROXYMETHYL)AMINOMETHANE) TRIS(HYDROXYMETHYL)AMINOMETHANE, ULTRAPURE >99.9% TRIHYDROXYMETHYLAMINOMETHANE(TRIS) 3-Propanediol, 2-amino-2-(hydroxymethyl) Tris(hydroxymethyl)aminomethane, ACS, 99.8-100.1% (Assay, dried basis) Tris(hydroxymethyl)aminomethane [for Electrophoresis] 1,1,1-tris(hydroxymethyl)-methanamin 1,1,1-tris(hydroxymethyl)-methylamin 1,1,1-tris(hydroxymethyl)methylamine 1,3-Propanediol,2-amino-2-(hydroxymethyl)- Tris(hydroxymethyl)amino-methan tris(hydroxymethyl)aminomethane(tris) Tris(hydroxymethyl)methanamine Tris, free base tris[bufferingagent] Tris-(hydroxymethyl)-aminomethane, buffer substance, reagent grade, ACS