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TRIS hydrochloride

TRIS hydrochloride Structure
CAS No.
1185-53-1
Chemical Name:
TRIS hydrochloride
Synonyms
TRIS-HCL;Tris-HCI;TRIZMA HYDROCHLORIDE;TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE;TRIS-HCL BUFFER;TROMETHAMINE HYDROCHLORIDE;Tris hydrochloric acid;TRIZMA HYDROCHLORIDE REAGENT GRADE;TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE;2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride
CBNumber:
CB2677770
Molecular Formula:
C4H12ClNO3
Molecular Weight:
157.6
MOL File:
1185-53-1.mol
MSDS File:
SDS
Modify Date:
2024/8/15 16:24:08
Request For Quotation

TRIS hydrochloride Properties

Melting point 150-152 °C
Boiling point 225℃[at 101 325 Pa]
Density 1.05 g/mL at 20 °C
vapor pressure 0Pa at 20℃
storage temp. room temp
solubility H2O: 4 M at 20 °C, clear, colorless
form crystalline
color clear colorless (40 % (w/w) in H2O) solution
PH Range 7.0 - 9.0
PH 2.5-4.0 (25℃, 4M in H2O)
pka 8.1(at 25℃)
Water Solubility SOLUBLE
Sensitive Hygroscopic
λmax λ: 260 nm Amax: 0.12
λ: 280 nm Amax: 0.08
BRN 3675235
Stability Stable. Incompatible with strong oxidizing agents, bases, moisture.
InChIKey QKNYBSVHEMOAJP-UHFFFAOYSA-N
LogP -3.6 at 20℃
CAS DataBase Reference 1185-53-1(CAS DataBase Reference)
EPA Substance Registry System 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1185-53-1)

SAFETY

Risk and Safety Statements

Symbol(GHS) 
GHS07
Signal word  Warning
Hazard statements  H315-H319-H335
Precautionary statements  P305+P351+P338
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  26-36
WGK Germany  3
RTECS  TY2900000
F  3
TSCA  Yes
HS Code  29221980
NFPA 704
1
2 1

TRIS hydrochloride price More Price(41)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich(India) T6666 Trizma® hydrochloride pH 3.5-5.0 (0.5?M in H2O), BioXtra, ≥99.0% (titration) 1185-53-1 50G ₹6928 2022-06-14 Buy
Sigma-Aldrich(India) T6666 Trizma® hydrochloride pH 3.5-5.0 (0.5?M in H2O), BioXtra, ≥99.0% (titration) 1185-53-1 250G ₹14613.75 2022-06-14 Buy
Sigma-Aldrich(India) T5941 Trizma® hydrochloride BioPerformance Certified, suitable for cell culture, ≥99.0% (titration) 1185-53-1 100G ₹10229.63 2022-06-14 Buy
Sigma-Aldrich(India) T6666 Trizma® hydrochloride pH 3.5-5.0 (0.5?M in H2O), BioXtra, ≥99.0% (titration) 1185-53-1 1KG ₹36436.95 2022-06-14 Buy
Sigma-Aldrich(India) T5941 Trizma® hydrochloride BioPerformance Certified, suitable for cell culture, ≥99.0% (titration) 1185-53-1 1KG ₹10760.05 2022-06-14 Buy
Product number Packaging Price Buy
T6666 50G ₹6928 Buy
T6666 250G ₹14613.75 Buy
T5941 100G ₹10229.63 Buy
T6666 1KG ₹36436.95 Buy
T5941 1KG ₹10760.05 Buy

TRIS hydrochloride Chemical Properties,Uses,Production

Description

Abbreviated as Tris HCl (TRIS hydrochloride), Tris (hydroxymethyl) aminomethane hydrochloride is an organic compound commonly utilized in buffer solutions such as TAE or TBE for electrophoresis gels. It boasts high solubility in water and offers utility within the pH range of 7.0-9.0. The compound is instrumental in preparing Laemmli buffer, one of the most widely used SDS-PAGE buffers, and also finds application in various custom running and loading buffers, frequently in combination with glycine and SDS. Lysis, the initial step of DNA extraction, involves a buffer containing Tris and EDTA (ethylenediaminetetraacetic acid). In this process, EDTA binds to divalent cations such as calcium and magnesium. Due to their role in maintaining cell membrane integrity, their removal by EDTA leads to membrane destabilization. Tris plays a key role as the primary buffering component, maintaining the pH of the buffer at a stable point, typically around 8.0. Additionally, Tris may interact with the LPS (lipopolysaccharide) in the membrane, contributing to further membrane destabilization. This underscores Tris HCl's significance in various laboratory procedures.

Chemical Properties

TRIS hydrochloride is a white crystalline powder that has a pKa of 8.08 at 25°C and pH range of 7.0 to 9.0 that coincides with the physiological pH of most living organisms because of which it is commonly used as a component of buffer solutions in biology, biochemistry and molecular biology applications. Tris salts are also used for crystallization of proteins at various pH values. Neither Tris base nor Tris hydrochloride by itself provide adequate buffering capacity. Generally the two need to be mixed together to provide a buffer with pH between 7 and 9 to provide adequate buffering.

Uses

TRIS hydrochloride is a high quality buffer component used in SDS-PAGE. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. It is also used in the robust generation of transgenic mice.

Uses

Tris Hydrochloride is a buffering medium for electrophoresis, molecular biology, and cell culture applications. It may be combined with Tris Base to simplify Tris buffer preparation. Tris Hydrochloride has been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs. In immunohistochemistry, Tris Hydrochloride has also been used with urea as a method for antigen retrieval.

Application

TRIS hydrochloride is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.

Preparation

The preparation of TRIS hydrochloride is as follows:After reacting 2-amino-1,3-propanediol (2.50 g, 27.44 mmol) and formic acid (1.26 g, 27.44 mmol) in 50 ml of water at room temperature for 3 hours, water was distilled off under reduced pressure, A liquid was obtained. By washing the obtained liquid,An ammonium formate salt (3.76 g, 27.44 mmol) was obtained as a colorless transparent liquid.

General Description

TRIS hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Biological Activity

Commonly used laboratory reagent

Purification Methods

Crystallise the salt from 50% EtOH, then from 70% EtOH. TRIS-hydrochloride is also available commercially in a highly pure state. Otherwise, recrystallise it from 50% EtOH, then 70% EtOH, and dry it below 40o to avoid risk of decomposition. [Beilstein 4 H 304.]

TRIS hydrochloride Preparation Products And Raw materials

Raw materials

Preparation Products

Glycine hydrochloride 2,2-DIMETHYL-1,3-DIOXAN-5-ONE

TRIS hydrochloride Suppliers

Global( 611)Suppliers
Supplier Tel Country ProdList Advantage Inquiry
ORGANO FINE CHEMICALS +91-91-22-42128300 +91-2242128300 Mumbai, India 258 58 Inquiry
R SCIENTIFIC +919000280374 Hyderabad, India 143 58 Inquiry
RYZE CHEMIE +91-9702966440 +91-9702966440 Maharashtra, India 335 58 Inquiry
Organo Fine Chemicals +91 (22) 2201-6968 / 2206-7521 New Delhi, India 266 60 Inquiry
Scientific OEM +91-22- 2343 7546 / 2341 3094 New Delhi, India 1996 38 Inquiry
ALPHA CHEMIKA +91-22-22061123 +91-22-66382501 Mumbai, India 1681 43 Inquiry
CHEMSWORTH +91-261-2397244 New Delhi, India 6707 30 Inquiry
FINAR LTD +91 - 2717 - 616717 New Delhi, India 660 58 Inquiry
A.J Chemicals 91-9810153283 New Delhi, India 6124 58 Inquiry
CLEARSYNTH LABS LTD. +91-22-45045900 Hyderabad, India 6351 58 Inquiry
Supplier Advantage
ORGANO FINE CHEMICALS 58
R SCIENTIFIC 58
RYZE CHEMIE 58
Organo Fine Chemicals 60
Scientific OEM 38
ALPHA CHEMIKA 43
CHEMSWORTH 30
FINAR LTD 58
A.J Chemicals 58
CLEARSYNTH LABS LTD. 58

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TRIS hydrochloride Spectrum

TRIS hydrochloride(1185-53-1)1HNMRTRIS hydrochloride(1185-53-1)IR1TRIS hydrochloride(1185-53-1)IR2TRIS hydrochloride(1185-53-1)Raman

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T-BAG WITH GRANULATE FOR 5L TSB 3-propanediol,2-amino-2-(hydroxymethyl)-hydrochloride trizmahydrochloride(trishydrochloride) Tris(2-amino-2-hydroxymethyl)-1,3-propanediol, hydrochloride Tris(hydroxymethyl)aminomethane hydrochloride, extra pure, 99+% TrisHCl,mole-readyform,molecularbiologygrade TRISHYDROXYMETHYLAMINOMETHANE HYDROCHLORIDE BIO-REFINED TRIS(HYDROXYMETHYL)AMINOMETHANEHYDROCHLORIDE,BIOTECHGRADE TRIS(HYDROXYMETHYL)AMINOMETHANEHYDROCHLORIDE,CRYSTAL,REAGENT tris(hydroxymethyl)aminomethane hydrochliride(TRIS.HCL) 2-Amino-2-(hydroxymethyl)propan-1,3-diolhydrochlorid Tris-(hydroxymethyl)aminomethane hydrochloride (TRIS-Hydrochloride) TRIS HYDROCHLORIDE ULTRAPURE TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE >99.5% TRIS(HYDROXYMETHYL) AMINOMETHANE HYDORCHLORIDE TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE extrapure AR TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride Trizma hydrchloride TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE, 99+%, EXTRA PURE 3-propanediol hydrochloride TRIZMA(R) HYDROCHLORIDE, BIOPERFORMA TRIZMA(R) HYDROCHLORIDE, REAGENT GRA TRIZMA HYDROCHLORIDE, FOR LUMINE TRIZMA HYDROCHLORIDE, FOR MOLECU Tris, Hydrochloride, ULTROL TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE TROMETHANE HCL TROMETHANE HYDROCHLORIDE TROMETAMOL HYDROCHLORIDE TRIS(HYDROXYMETHYL)AMINOMETHANE HCL BUFFER SUBST.PH 7.5* TRIS HCL 1 M STOCK SOLUTION PH 7.40 TRIZMA HCL, BIOTECHNOLOGY PERFORMANCE CE RTIFIED TRIS(HYDROXYMETHYL)AMINOMETHANE HCL 99% TRIS(HYDROXYMETHYL)AMINOMETHANE ULTRA PURE GRADE 99.5+% TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE（TRIS.HCL） 1MTrisHydrochlorideSolution,Ph9.0,Sterile Tris-HclTris(Hydroxymethyl)AminomethaneHydrochloride 1MTrisHydrochlorideSolution,Ph7.0 1MTrisHydrochlorideSolution,Ph10.0,Sterile Tris(Hydromethyl)AminomethaneHcl 1MTrisSolution,Ph8.0 Tris-HydrochlorideGr Tris(hydroxymethyl)aminomethane hydrochloride, 99+% Tris-HCl （Hydrochloride） Tris HCl (Tris hydrochloride) 2-AMINO-2-(HYDROXYMETHYL)-1,3-PROPANEDIOL, HYDROCHLORIDE SPECTRIS(R) HYDROCHLORIDE SPECTRIS(TM) HYDROCHLORIDE MOLE-READY-TRIS 1,3-Propanediol,2-amino-2-(hydroxymethyl)-,hydrochloride TRIS(HYDROXYMETHYL)AMINO-METHANE.HYDROCHLORIDE RES.GRADE TRIS(HYDROXYMETHYL)AMINOETHANE* HYDROCHLORIDE, MOLECULAR BIOLOGY GRADE Trizma hydrochloride,TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride Tris-HCl, molecular biology grade TRIZMA(R) HYDROCHLORIDE TRISMAT(TM) HYDROCHLORIDE TRISMAT(R) HYDROCHLORIDE TRIS HYDROCHLORIDE