α-Chymotrypsin
| Melting point | 127°C |
|---|---|
| Density | 1.37[at 20℃] |
| vapor pressure | 0Pa at 25℃ |
| storage temp. | -20°C |
| solubility | Reconstitute in 1mM HCl. Soluble at 10mg/ml in 1mM HCl. 2mM calcium chloride serves as a stabilizer. Store aliquoted solutions at -20°C for up to a week. |
| form | salt-free, lyophilized powder |
| color | white |
| Water Solubility | 125g/L at 25℃ |
| Merck | 13,2282 |
| LogP | -1.3 at 20℃ |
| EPA Substance Registry System | Chymotrypsin (9004-07-3) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |   GHS07,GHS08 |
|---|---|
| Signal word | Danger |
| Hazard statements | H315-H317-H319-H334-H335 |
| Precautionary statements | P280-P302+P352-P305+P351+P338 |
| Hazard Codes | Xn,B |
| Risk Statements | 36/37/38-42/43-42 |
| Safety Statements | 26-36-36/37-24-22 |
| WGK Germany | 3 |
| RTECS | GC3050000 |
| F | 3-10 |
| TSCA | Yes |
| HS Code | 35079090 |
α-Chymotrypsin price More Price(22)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich(India) | SRP6509 | Chymotrypsin from human pancreas ≥95% (SDS-PAGE) | 9004-07-3 | 100μG | ₹37540.2 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | C9134 | α-Chymotrypsin−Agarose from bovine pancreas lyophilized powder, 2,000-3,500?units/g agarose (One ml gel will yield 65-120?units) | 9004-07-3 | 50UNITS | ₹26906.4 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | CHY5S | α-Chymotrypsin from bovine pancreas ≥40?units/mg protein, vial of 5?mg | 9004-07-3 | 10VIALS | ₹28660.2 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | C9134 | α-Chymotrypsin−Agarose from bovine pancreas lyophilized powder, 2,000-3,500?units/g agarose (One ml gel will yield 65-120?units) | 9004-07-3 | 100UNITS | ₹48129.6 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | C7762 | α-Chymotrypsin from bovine pancreas Type I-S, essentially salt-free, lyophilized powder | 9004-07-3 | 5MG | ₹3463.2 | 2022-06-14 | Buy |
α-Chymotrypsin Chemical Properties,Uses,Production
Chemical Properties
Lyophilized powder, dialyzed
Uses
α-Chymotrypsin from bovine has been used in a study to inform proteasome inhibition in order to advance anticancer research. α-Chymotrypsin from bovine has also been used in a study that functionalized surface anchored poly(methylhydrosiloxane) thin films on oxidized silicon wafers.
General Description
Chymotrypsin (Chymar) is extractedfrom mammalian pancreas and is used in cataractsurgery. A dilute solution is used to irrigate the posteriorchamber of the eye to dissolve the fine filaments that holdthe lens.
Mechanism of action
The general mechanism for chymotrypsin is the classic serine protease mechanism. Hydrolytic proteolysis by α-chymotrypsin begins with an initial nucleophilic attack on the peptide bond by Ser 195, activated by deprotonation by His 57. This leads to forming a tetrahedral intermediate, stabilized by the amide groups of Ser 195 and Gly 193. The subsequent collapse of this intermediate, assisted by protonation of the leaving group by His 57 and Asp 102, leads to an acyl-enzyme intermediate. Activation of a water molecule by His 57 and Asp 102 facilitates hydrolysis of this intermediate, resulting in the reformation of the catalytically active serine residue and the release of the product facilitated by protonation with His 57.
Purification Methods
α-Chymotrypsin is crystallised twice from four-tenths saturated ammonium sulfate solution, then dissolved in 1mM HCl and dialysed against 1mM HCl at 2-4o. The solution is stored at 2o [Lang et al. J Am Chem Soc 80 4923 1958].
References
[1] R J Schilling, A K Mitra. “Degradation of insulin by trypsin and alpha-chymotrypsin.” Pharmaceutical Research 8 6 (1991): 721–7.
α-Chymotrypsin Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | Inquiry |
|---|---|---|---|---|---|
| Meteoric Biopharmaceuticals Pvt. Ltd. | +91-8511121361 +91-8511121361 | Mumbai, India | 71 | 58 | Inquiry |
| Symbio Generrics (I) Pvt Ltd | +91-8121318328 +91-9099033709 | Karnataka, India | 22 | 58 | Inquiry |
| S D Fine Chem Limited | +91-9323715856 +91-9323715856 | Maharashtra, India | 368 | 58 | Inquiry |
| Beloorbayir Biotech Ltd | +91-8792661159 +91-8867683718 | Karnataka, India | 25 | 58 | Inquiry |
| Apollo ingredients Pvt Ltd | +91-7219888886 | Maharashtra, India | 31 | 58 | Inquiry |
| CLEARSYNTH LABS LTD. | +91-22-45045900 | Hyderabad, India | 6351 | 58 | Inquiry |
| TCI Chemicals (India) Pvt. Ltd. | 1800 425 7889 | New Delhi, India | 6778 | 58 | Inquiry |
| Otto Chemie Pvt. Ltd. | +91 9820041841 | Mumbai, India | 5873 | 58 | Inquiry |
| Sisco Research Laboratories Pvt. Ltd. | +91-22-4268 5800 | Mumbai, India | 4317 | 58 | Inquiry |
| Pharmaffiliates Analytics and Synthetics P. Ltd | +91-172-5066494 | Haryana, India | 6773 | 58 | Inquiry |
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