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PIPES

PIPES Structure
CAS No.
5625-37-6
Chemical Name:
PIPES
Synonyms
PIPES FREE ACID;1,4-Piperazinebis(ethanesulphonic acid);PIPES;PIPES,98%;PIPES0215;Pipes AR 98%;PIPES【Dotite】;Pipes(Buffer);PIPES solution;MOLYBDENE STAND
CBNumber:
CB4435134
Molecular Formula:
C8H18N2O6S2
Molecular Weight:
302.37
MOL File:
5625-37-6.mol
MSDS File:
SDS
Modify Date:
2024/8/11 15:28:25
Request For Quotation

PIPES Properties

Melting point >300 °C (lit.)
Density 1.4983 (rough estimate)
refractive index 1.6300 (estimate)
storage temp. Sealed in dry,Room Temperature
solubility 0.1 M NaOH: 0.25 g/mL, clear, colorless
form Micro-Crystalline Powder
color White
pka 6.8(at 25℃)
PH Range 6.1 - 7.5
Water Solubility Soluble in water.
λmax λ: 260 nm Amax: 0.055
λ: 280 nm Amax: 0.040
Merck 14,7479
BRN 817713
InChIKey XWGRYGKFRRLUQQ-UHFFFAOYSA-N
LogP -2.7 at 20℃
CAS DataBase Reference 5625-37-6(CAS DataBase Reference)
EPA Substance Registry System 1,4-Piperazinediethanesulfonic acid (5625-37-6)

SAFETY

Risk and Safety Statements

Symbol(GHS) 
GHS07
Signal word  Warning
Hazard statements  H315-H319-H335
Precautionary statements  P261-P271-P280
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  22-24/25-37/39-26
WGK Germany  3
TSCA  Yes
HS Code  29335995
NFPA 704
1
1 0

PIPES price More Price(27)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich(India) P6757 PIPES ≥99% (titration) 5625-37-6 25G ₹6419.23 2022-06-14 Buy
Sigma-Aldrich(India) P8203 PIPES BioXtra, ≥99% (titration) 5625-37-6 50G ₹16454 2022-06-14 Buy
Sigma-Aldrich(India) RDD004 PIPES anhydrous, free-flowing, Redi-Dri?, ≥99% 5625-37-6 1KG ₹64441.23 2022-06-14 Buy
Sigma-Aldrich(India) P8203 PIPES BioXtra, ≥99% (titration) 5625-37-6 250G ₹48918.18 2022-06-14 Buy
Sigma-Aldrich(India) P6757 PIPES ≥99% (titration) 5625-37-6 100G ₹17915.38 2022-06-14 Buy
Product number Packaging Price Buy
P6757 25G ₹6419.23 Buy
P8203 50G ₹16454 Buy
RDD004 1KG ₹64441.23 Buy
P8203 250G ₹48918.18 Buy
P6757 100G ₹17915.38 Buy

PIPES Chemical Properties,Uses,Production

Description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Chemical Properties

White/clear crystalline powder

Uses

A buffering agent with a pKa near physiological pH.PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.
Additional forms available: PIPES, Sesquisodium Salt ; PIPES dipotassium salt; PIPES disodium salt; PIPES, Sodium Salt. Buffers can be prepared by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.

Application

Glutaraldehyde fixation of plant and animal tissue samples can cause loss of lipid, leading to apparent morphological changes. Lipid loss and artifacts were minimized when PIPES was used to buffer the glutaraldehyde fixative.
Alkaline phosphatase activity is lost selectively from certain rat hepatocyte organelles when fixed for ultracytochemistry with cacodylate-buffered glutaraldehyde. When PIPES was used as buffer, retention of activity was 60% greater.
Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer.

General Description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Purification Methods

Purify PIPES from boiling water (maximum solubility is about 1g/L) or as described for ADA [N-(2-acetamido)iminodiacetic acid above]. [Good et al. Biochemistry 5 469 1966, Beilstein 23/12 V 380.]

PIPES Preparation Products And Raw materials

Raw materials

Piperazine Sodium 2-chloroethanesulfonate monohydrate

Preparation Products

PIPES Suppliers

Global( 435)Suppliers
Supplier Tel Country ProdList Advantage Inquiry
JSK Chemicals +919879767970 Gujarat, India 3756 58 Inquiry
CLEARSYNTH LABS LTD. +91-22-45045900 Hyderabad, India 6351 58 Inquiry
Maas Pharma Chemicals +91-9958666224 Delhi, India 1607 58 Inquiry
A.J Chemicals 91-9810153283 New Delhi, India 6124 58 Inquiry
Otto Chemie Pvt. Ltd. +91 9820041841 Mumbai, India 5873 58 Inquiry
Alfa Aesar 1 800 209 7001 Maharashtra, India 6913 58 Inquiry
Sisco Research Laboratories Pvt. Ltd. +91-22-4268 5800 Mumbai, India 4317 58 Inquiry
Triveni chemicals 08048762458 New Delhi, India 6093 58 Inquiry
LOBA CHEMIE PVT.LTD. 91-22-6663 6699 Mumbai, India 3077 58 Inquiry
Central Drug House(P) Ltd. 91-11-49404040 New Delhi, India 6160 58 Inquiry
Supplier Advantage
JSK Chemicals 58
CLEARSYNTH LABS LTD. 58
Maas Pharma Chemicals 58
A.J Chemicals 58
Otto Chemie Pvt. Ltd. 58
Alfa Aesar 58
Sisco Research Laboratories Pvt. Ltd. 58
Triveni chemicals 58
LOBA CHEMIE PVT.LTD. 58
Central Drug House(P) Ltd. 58

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PIPES Spectrum

PIPES(5625-37-6)1HNMRPIPES(5625-37-6)IR1PIPES(5625-37-6)IR2PIPES(5625-37-6)Raman

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PIPES,98% PIPES Piperazine-1,4-bisethanesulfonic acid PIPES Vetec(TM) reagent grade, >=99% ABTS DIAMMONIUM SALT ULTRA PURE GRADE MOLYBDENE STAND MOLYBDENUM STAND PIPES BIOXTRA, FOR MOLECULAR BIOLOGY PIPES, BIOPERFORMANCE CERTIFIED& LABOTEST-BB LT00138111 1,4-PIPERAZINEBIS(ETHANESULFONIC ACID) 1,4-PIPERAZINEDIETHANESULFONIC ACID PIPERAZINE-1,4-BIS(2-ETHANESULFONIC ACID) PIPERAZINE-NN'-BIS-2-ETHANESULPHONIC ACID PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID) PIPES TIMTEC-BB SBB008973 2,2-(piperazine-1,4-diyl)bis(ethanesulphonic) acid PIPES Piperazine-N,N-bis(2-ethanesulfonic acid) PIPES, Free Acid, MB Grade (1.12030) PIPES, Free Acid, ULTROL Grade PIPES, Molecular Biology Grade Piperazine-N,N-bis(2-ethanesulfonic acid), Molecular Biology Grade Piperazine-1,4-bis(ethanesulfonic acid) PIPERAZINE-1 4-BIS(2-ETHANESULFONIC ACI& PIPES FREE ACID SIGMAULTRA PIPES FREE ACID, BIOTECHNOLOGY PERFORMAN CE CERTIFIE PIPERAZINE-1,4-BIS(2-ETHANESULFONIC ACID ), F. MOL.BIOLOGY SODIUM BIS(TRIMETHYLSILYL)AMIDE SOLUTION, WACKER QUALITY Pipes(Buffer)-(Piperazine-N,N-Bis-2-EtheneSulfonicAcid) 1,4-Piperazinediethanesulfonic acid(PIPES) Piperazine-1,4-diethyl acid PIPES (Piperazin-1,4-bis(2-ethansulfonic acid) Piperazine-N,N'-bis-2-ethanesulphonic acid ,99% PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID) ANALYTICAL GRADE PIPERAZINE-N,N'-BIS(2-ETHANE SULFONIC ACID)MOLECULAR BIOLOGY GRADE PIPES,1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid) PIPES free acid, molecular biology grade 1,4-Piperazinedietha PIPES, Free Acid, ULTROL PIPES (PIPERAZINE-1,4-BIS(2-ETHANESULFONIC ACID)) PIPES ULTRAPURE PIPERAZINE-N,N-BIS(2-ETHANESULFONICACID)(PIPES) Piperazine-N,N'-bis-(2-ethanesulphonic acid) ultrapure >99.5% 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,Nμ-bis(2-ethanesulfonic acid) 1,4-Piperazinebis(ethansulfonic acid) 2,2'-(Piperazine-1,4-diyl)bis(ethanesulfonic) acid 2-[4-(2-Sulfoethyl)piperazin-1-yl]ethanesulfonic acid Pipes, 98.5+%, for biochemistry PIPES, for biochemistry 2,2'-(1,4-Piperazinediyl)bis(ethanesulfonic acid) 2,2'-(1,4-Piperazinediyl)bisethanesulfonic acid PIPES【Dotite】 2,2'-(Piperazine-1,4-diyl)diethanesulfonic acid Pipes(Buffer) PIPES, for biochemistry, 98.5+% Piperazine-1,4-bis(2-ethanesulfonic acid)(PIPES) 1,4-Piperazinediethanesulfonic PIPES,BIOLOGICALBUFFER 1;4-PIPERAZINEDIETHANESULFONIC ACID;PIPERAZINE-N;N'-BIS(2-ETHANESULFONIC ACID);2;2'-(PIPERAZINE-1;4-DIYL)DIETHANESULFONIC ACID