BCECF-AM

BCECF-AM Structure
CAS No.
117464-70-7
Chemical Name:
BCECF-AM
Synonyms
BCECF-AM;BCECF--1M;BCECF--1M 1MG;Bcecf-AmSolution;bcecf-acetoxymethyl;BCECF-Acetoxymethyl ester;BCECF/AM - CAS 117464-70-7 - Calbiochem;BCECF.AM ESTER, 1 MG/ML IN*ANHYDROUS DMSO;2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN;2',7'-bis(2-carboxyethyl)-5(6)-*carboxyfluorescei
CBNumber:
CB5111514
Molecular Formula:
C42H40O21
Molecular Weight:
880.75
MOL File:
117464-70-7.mol
Modify Date:
2024/7/2 8:55:09

BCECF-AM Properties

Boiling point 692.41°C (rough estimate)
Density 1.2837 (rough estimate)
refractive index 1.6500 (estimate)
Flash point 85 °C
storage temp. -20°C
form White to off-white solid
color Orange to red
CAS DataBase Reference 117464-70-7(CAS DataBase Reference)

SAFETY

Risk and Safety Statements

Symbol(GHS) 
GHS07
Signal word  Warning
Hazard statements  H319-H315-H335
Precautionary statements  P264-P280-P302+P352-P321-P332+P313-P362-P264-P280-P305+P351+P338-P337+P313P
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  22-26-36
WGK Germany  3

BCECF-AM price More Price(1)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich(India) 216254 BCECF/AM - CAS 117464-70-7 - Calbiochem A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator 117464-70-7 1MG ₹15410.01 2022-06-14 Buy
Product number Packaging Price Buy
216254 1MG ₹15410.01 Buy

BCECF-AM Chemical Properties,Uses,Production

Description

BCECF AM ester is a widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF AM can also be used to investigate intracellular changes in other ions, including potassium.

Chemical Properties

pale yellow to yellow powder

Uses

Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.

General Description

Material is an isomeric mixture of lactones, acetates, and AM esters

Biological Activity

Non-charged, non-fluorescent pH indicator. Readily and rapidly diffuses through cell membranes.

BCECF-AM Preparation Products And Raw materials

Raw materials

Preparation Products

BCECF-AM Suppliers

Global( 122)Suppliers
Supplier Tel Country ProdList Advantage Inquiry
CONIER CHEM AND PHARMA LIMITED +8618523575427 China 49391 58 Inquiry
TargetMol Chemicals Inc. +1-781-999-5354 +1-00000000000 United States 19892 58 Inquiry
Alfa Chemistry United States 24072 58 Inquiry
LEAPCHEM CO., LTD. +86-852-30606658 China 43348 58 Inquiry
Aladdin Scientific +1-+1(833)-552-7181 United States 57511 58 Inquiry
Aladdin Scientific +1-+1(833)-552-7181 United States 52927 58 Inquiry
Shanghai Aladdin Bio-Chem Technology Co.,LTD 400-400-6206333 18521732826 China 25013 65 Inquiry
Beijing Ouhe Technology Co., Ltd 010-82967028 13552068683 China 12458 60 Inquiry
InvivoChem 13549236410 China 6749 58 Inquiry
Henan Alpha Chemical Co., Ltd. 0371-55013243 15324716602 China 10010 58 Inquiry
2',7'-BIS-(2-CARBOXYETHYL)-5-(AND-6)-CARBOXYFLUORESCEIN, ACETOXYMETHYL ESTER 2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN 2',7'-BIS(2-CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER 2',7'-BIS-(CARBOXYETHYL)-5(6')-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER 2',7'-bis(2-carboxyethyl)-5(6)-*carboxyfluorescei BCECF.AM ESTER, 1 MG/ML IN*ANHYDROUS DMSO Bcecf-AmSolution 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester mixed isomers bcecf-acetoxymethyl 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein AM ester BCECF-AM solution, Spiro(isobenzofuran-1(3H),9μ-(9H)xanthene)-2μ,7μ-dipropanoic acid 2',7'-Bis(2-acetoxymethoxycarbonylethyl)-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid acetoxymethyl ester 2,7-Bis[2-(acetoxymethoxycarbonyl)ethyl]-3,6-dihydroxy-3'-oxospiro[9H-xanthene-9,1'(3'H)-isobenzofuran]-6'-carboxylic acid acetoxymethyl ester 2',7'-Biscarboxyethyl-6-carboxyfluorescein acetoxymethyl ester BCECF-AM 3',6'-bis(Acetyloxy)-5(or6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoicacid2',7'-bis[(acetyloxy)methyl]ester BCECF-Acetoxymethyl ester BCECF--1M BCECF--1M 1MG SPIRO(ISOBENZOFURAN-1(3H),9'-(9H)XANTHENE)-2',7'-DIPROPANOIC ACID 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxyMet BCECF/AM - CAS 117464-70-7 - Calbiochem 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethy 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester 117464-70-7 C42H40O21 C39H36O19 Ion Channels Monovalent Ion Channels Voltage-gated Ion Channels Cell Signaling and Neuroscience BioChemical Cell Biology Ion Probes