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BCECF-AM

BCECF-AM Structure
  • ₹15410.01
  • Product name: BCECF-AM
  • CAS: 117464-70-7
  • MF: C42H40O21
  • MW: 880.75
  • EINECS:
  • MDL Number:MFCD00036969
  • Synonyms:2',7'-BIS-(2-CARBOXYETHYL)-5-(AND-6)-CARBOXYFLUORESCEIN, ACETOXYMETHYL ESTER;2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN;2',7'-BIS(2-CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER;2',7'-BIS-(CARBOXYETHYL)-5(6')-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER;2',7'-bis(2-carboxyethyl)-5(6)-*carboxyfluorescei;BCECF.AM ESTER, 1 MG/ML IN*ANHYDROUS DMSO;Bcecf-AmSolution;2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester mixed isomers
Price1 Properties Safety Information Description
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Brand

  • Sigma-Aldrich(India)

Package

  • 1MG
  • ManufacturerSigma-Aldrich(India)
  • Product number216254
  • Product descriptionBCECF/AM - CAS 117464-70-7 - Calbiochem A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator
  • Packaging1MG
  • Price₹15410.01
  • Updated2022-06-14
  • Buy
Manufacturer Product number Product description Packaging Price Updated Buy
Sigma-Aldrich(India) 216254 BCECF/AM - CAS 117464-70-7 - Calbiochem A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator 1MG ₹15410.01 2022-06-14 Buy

Properties

Boiling point :692.41°C (rough estimate)
Density :1.2837 (rough estimate)
refractive index :1.6500 (estimate)
Flash point :85 °C
storage temp. :-20°C
form :White to off-white solid
color :Orange to red
CAS DataBase Reference :117464-70-7(CAS DataBase Reference)

Safety Information

Symbol(GHS): GHS hazard pictograms
Signal word: Warning
Hazard statements:
Code Hazard statements Hazard class Category Signal word Pictogram P-Codes
H315 Causes skin irritation Skin corrosion/irritation Category 2 Warning GHS hazard pictograms P264, P280, P302+P352, P321,P332+P313, P362
H319 Causes serious eye irritation Serious eye damage/eye irritation Category 2A Warning GHS hazard pictograms P264, P280, P305+P351+P338,P337+P313P
H335 May cause respiratory irritation Specific target organ toxicity, single exposure;Respiratory tract irritation Category 3 Warning GHS hazard pictograms
Precautionary statements:
P264 Wash hands thoroughly after handling.
P264 Wash skin thouroughly after handling.
P280 Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352 IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P321 Specific treatment (see … on this label).
P332+P313 IF SKIN irritation occurs: Get medical advice/attention.
P362 Take off contaminated clothing and wash before reuse.

Description

BCECF-Acetoxymethyl (BCECF-AM) ester is a widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF-AM ester can also be used to investigate intracellular changes in other ions, including potassium.

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