Neutral Red
- CAS No.
- 553-24-2
- Chemical Name:
- Neutral Red
- Synonyms
- Neutral Red;N2,N2,7-TriMethylphenazine-2,8-diaMine hydrochloride;Kernechrot;C.I. 50040;neutralredw;Basic Red 5;c.i.basicred5;michromeno.226;cervenneutralni;cervenzasadita5
- CBNumber:
- CB7853810
- Molecular Formula:
- C15H17ClN4
- Molecular Weight:
- 288.78
- MOL File:
- 553-24-2.mol
- MSDS File:
- SDS
- Modify Date:
- 2024/3/8 18:31:40
| Melting point | 290 °C (dec.) (lit.) |
|---|---|
| Boiling point | 441.31°C (rough estimate) |
| Density | 1.1590 (rough estimate) |
| refractive index | 1.6110 (estimate) |
| storage temp. | Store at +5°C to +30°C. |
| solubility | H2O: soluble10mg/mL |
| form | Solid |
| Colour Index | 50040 |
| pka | 6.7, 7.4(at 25℃) |
| color | Very dark green |
| PH Range | 6.8(red)-8(yellow) |
| PH | 3.1 (10g/l, H2O, 20℃) |
| Odor | Odorless |
| Water Solubility | 50 g/L |
| λmax | 540nm, 533nm, 544nm, 529nm, 454nm |
| Merck | 14,6488 |
| BRN | 3918740 |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| Biological Applications | Detecting pathogens,bacterial infections; treating age-related macular degeneration,burns,cancer,diabetes,obesity,fungal infections,viral diseases |
| Major Application | Fuel cell power generation system, liquid crystal displays, solor cells, sensors, thermochromic materials, coloring wood, detergents, assessment of tobacco smoke, cosmetics, detect bacterial infections, multidrug resistance inhibitors, treatment of burns, endodontic, diabetes, obesity, cancer, age-related macular degeneration, viral diseases |
| CAS DataBase Reference | 553-24-2(CAS DataBase Reference) |
| EPA Substance Registry System | Neutral Red (553-24-2) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|---|---|
| Signal word | Warning |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P280-P302+P352 |
| Hazard Codes | Xn,Xi |
| Risk Statements | 68-36/37/38 |
| Safety Statements | 36/37-37/39-36-26-24/25 |
| WGK Germany | 3 |
| RTECS | SG1400000 |
| TSCA | Yes |
| HS Code | 32041300 |
| Toxicity | LD50 intraperitoneal in mouse: 432mg/kg |
Neutral Red price More Price(30)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich(India) | N7005 | Neutral Red Dye content ≥90?% | 553-24-2 | 1G | ₹4451.1 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | N7005 | Neutral Red Dye content ≥90?% | 553-24-2 | 5G | ₹15628.8 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | N7005 | Neutral Red Dye content ≥90?% | 553-24-2 | 25G | ₹51404.1 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | N4638 | Neutral Red powder, BioReagent, suitable for cell culture | 553-24-2 | 1G | ₹5810 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | N4638 | Neutral Red powder, BioReagent, suitable for cell culture | 553-24-2 | 5G | ₹17450 | 2022-06-14 | Buy |
Neutral Red Chemical Properties,Uses,Production
Chemical Properties
dark green powder
Uses
Neutral Red is a common histology stain used to determine cell viability. It is also used as indicator for nitrogen determination; pH indicator; Dyes and metabolites.
Preparation
(a) N,N-dimethyl-4-nitrosobenzenamine?and 4-Methylbenzene-1,3-diamine???reaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine?, and 4-Methylbenzene-1,3-diamine mixture.
Application
Transition of pH Indicator: pH 6.8 - 8.0, red to yellow. Basic red 5 is a dye used in histology for staining nuclei. It is also a pH indicator. As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains it and lactose. Acidification by lactic acid turns on the Basic red 5. Therefore, colonies formed by bacteria that ferment lactose into lactic acid will turn red and stand out from the pale colonies of non-lactose-fermenting bacteria. Basic red 5 is also used in cell viability tests based on the ability to incorporate and bind it.
Definition
ChEBI: Neutral red is a hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0. It has a role as a dye, an acid-base indicator and a two-colour indicator. It contains a neutral red(1+).
General Description
Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.
Staining Procedures
Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.
Purification Methods
In aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.]
Neutral Red Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | Inquiry |
|---|---|---|---|---|---|
| Arnish Laborates Pvt Ltd | +91 7208475595 | Maharashtra, India | 187 | 58 | Inquiry |
| GSP Dyes and Chemicals Pvt.Ltd | +91-22-28144802 +91-2228144802 | Maharashtra, India | 79 | 58 | Inquiry |
| Anubhav Business Corporation | +91-2228014519 +91-2228014999 | Maharashtra, India | 119 | 58 | Inquiry |
| Esjay Chemicals | +919820452929 | Maharashtra, India | 110 | 58 | Inquiry |
| JSK Chemicals | +919879767970 | Gujarat, India | 3756 | 58 | Inquiry |
| B R D H SCIENTIFIC SUPPLIERS | 08047642302 | Nagpur, India | 46 | 58 | Inquiry |
| Anand Agencies | 91-20-24454597 | Maharashtra, India | 2337 | 58 | Inquiry |
| THOMAS BAKER (CHEMICALS) PVT. LTD. | +91-22-22811264 | New Delhi, India | 86 | 47 | Inquiry |
| Asiel Chempharma | 91-9920082277 | Maharashtra, India | 119 | 58 | Inquiry |
| Mala International | 08068442326Ext 398 | Mumbai, India | 165 | 58 | Inquiry |
| Supplier | Advantage |
|---|---|
| Arnish Laborates Pvt Ltd | 58 |
| GSP Dyes and Chemicals Pvt.Ltd | 58 |
| Anubhav Business Corporation | 58 |
| Esjay Chemicals | 58 |
| JSK Chemicals | 58 |
| B R D H SCIENTIFIC SUPPLIERS | 58 |
| Anand Agencies | 58 |
| THOMAS BAKER (CHEMICALS) PVT. LTD. | 47 |
| Asiel Chempharma | 58 |
| Mala International | 58 |