钙黄绿素乙酰甲酯
中文名称 | 钙黄绿素乙酰甲酯 |
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中文同义词 | 钙黄绿素,AM;黄绿素-AM;细胞染色剂黄绿素-AM;钙黄绿素, AM *超级纯*;钙黄绿素乙酰甲酯;Calcein,AM,≥96.0% (HPLC)用于荧光分析 (1mg 1119.00);钙黄绿素乙酰甲酯 CALCEIN, AM,≥96.0% (HPLC);CELLSTAIN CALCEIN AM SOLUTION(1MM/L DMSO) (3',6'-DI(O-ACETYL)-4',5'-BIS[N,N-BIS(CARBOXYMETHY |
英文名称 | CALCEIN-AM |
英文同义词 | 3',6'-Di(O-acetyl)-2',7'-bis[N,N-bis(carboxymethyl)aminomethyl]fluoresceintetraacetoxymethylester,DMSOsolution;Calcein AM solution;CALCEIN, AM;CAL-AM;4',5'-Bis(N,N-bis(carboxymethyl)aminomethyl)fluorescein acetoxymethyl ester;Glycine, N,N'-((3',6'-bis(acetyloxy)-3-oxospiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-diyl)bis(methylene))bis(N-(2-((acetyloxy)methoxy)-2-oxoethyl)-, bis((acetyloxy)methyl) ester;Calcein AcetoxyMethyl Ester;N,N'-[[3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(Methylene)]bis[N-[2-[(acetyloxy)Methoxy]-2-oxoethyl]glycine 1,1'-Bis[(acetyloxy) Methyl] Ester;NSC 689290 |
CAS号 | 148504-34-1 |
分子式 | C46H46N2O23 |
分子量 | 994.86 |
EINECS号 | 200-664-3 |
相关类别 | 荧光探针,标记,颗粒和染色;荧光染料;细胞生物学试剂;生化试剂;Amines;Aromatics;Fluorescent Labels & Indicators;Heterocycles;Intermediates & Fine Chemicals;Pharmaceuticals;Xanthene;CCalcium Signaling Research Tools;Calcium Probes;Fluorescent;Stains and Dyes;Stains&Dyes, A to |
Mol文件 | 148504-34-1.mol |
结构式 |
钙黄绿素乙酰甲酯 性质
熔点 | >250℃ |
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沸点 | 982.7±65.0 °C(Predicted) |
密度 | 1.49±0.1 g/cm3(Predicted) |
折射率 | n |
闪点 | 85 °C |
储存条件 | -20°C |
溶解度 | DMSO:可溶10mg/mL,澄清,无色至浅黄色 |
酸度系数(pKa) | 2.66±0.50(Predicted) |
形态 | 粉末 |
颜色 | 无色 |
最大波长(λmax) | < 300 nm |
生物领域应用 | Calcium indicators; zinc indicators; cytotoxicity assays; apoptosis assays; viability assays; labile iron pool assays; chemotaxis probes; cell adhesion probes; mitochondrial probes; P-glycoprotein probes;multi-drug resistance probes;treating atherosclerosis,cancer;ischemic disease |
The calcein-AM dye used to stain the living cells is shown to have a low spontaneousleakage rate less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM has a linear relationship with measured fluorescence values. NK cells, LAKs, and CTLs are readily detectable by this microtest. Quantitation of killing and kinetic analysis is readily performed with the test system. Calcein-AM is pH independent, better retained and more photostable. In addition, the high level of intracellular retention of calcein-AM and its low-level release after incorporation exclude possible cell-monolayer labeling and allow its use in a cell-cell interaction assay. Moreover, the bright fluorescence can easily be detected and measured by a microplate fluorescence reader. Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with intact plasma membrane. From dying or damaged cells with compromised membrane integrity or from cells expressing multidrug resistance protein (MRP), unhydrolyzed substrates and their fluorescent products are rapidly extruded from cells. The calcein-AM assay has been used to assess the cell viability, cytotoxicity and tp quantitate apoptosis.
Calcein-AM is found to be suitable for in vivo studies, because it has no deleterious effects on cell function and is, indeed, a marker of cell viability.
提供商 | 语言 |
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