Метиленовый голубой химические свойства, назначение, производство
Химические свойства
Methylene blue is a heterocyclic aromatic chemical compound with the molecular formula C16H18N3SCl. It has many uses in a range of different fields, such as biology and chemistry. At room temperature it appears as a solid, odorless, dark green powder, that yields a blue solution when dissolved in water. The hydrated form has 3 molecules of water per molecule of methylene blue. Methylene blue should not be confused with methyl blue, another histology stain, new methylene blue, nor with the methyl violets often used as pH indicators.
Использование
Methylene Blue, 1% w/v aqueous solution is used as a dye in different staining procedures viz. Wright's stain and Jenner's stain. It also serves as an indicator and medicine. Further, it is used to examine RNA and DNA under the microscope or in a gel. It is widely used as a redox indicator in analytical chemistry. It is also used in the sulfide analysis.
Определение
ChEBI: An organic chloride salt having 3,7-bis(dimethylamino)phenothiazin-5-ium as the counterion. A commonly used dye that also exhibits antioxidant, antimalarial, antidepressant and cardioprotective properties.
Общее описание
Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.
Опасность
Toxic by ingestion.
Биологическая активность
Biological stain and redox indicator. Inhibits tau filament formation (IC 50 = 1.9 μ M). Also inhibits soluble guanylyl cyclase.
Клиническое использование
Methylene blue is 3,7-bis(dimethylamino)-phenazathioniumchloride (Urised). The compound occurs as a dark greencrystalline powder with a metallic appearance that is solublein water (1:25) and alcohol (1:65).
Methylene blue has weak antiseptic properties that makeit useful for the treatment of cystitis and urethritis. The actionof methylene blue is considered to be bacteriostatic.The compound colors the urine and stool blue green.
Профиль безопасности
Poison by ingestion,intraperitoneal, intravenous, and subcutaneous routes.Human systemic effects: cyanosis, blood changes.Experimental reproductive effects. Mutation datareported. When heated to decomposition it emits verytoxic fumes of NOx, SOx, and
Методы очистки
Crystallise the chloride from 0.1M 664 2HCl (16mL/g), the crystals are separated by centrifugation, washed with chilled EtOH and diethyl ether, and dried under vacuum. Crystallise it from 50% aqueous EtOH, wash it with absolute EtOH, and dry it at 50-55o for 24hours. It has also been crystallised from *benzene/MeOH (3:1). It has been salted out with NaCl from a commercial concentrated aqueous solution, then crystallised from water, and dried at 100o in an oven for 8-10hours. [Beilstein 27 III/IV 5152.]
Метиленовый голубой препаратная продукция и сырье
сырьё
препарат