Biotinyl tyramide
- CAS No.
- 41994-02-9
- Chemical Name:
- Biotinyl tyramide
- Synonyms
- Biotinyl TSA;Biotin Tyramide;D-(+)-Biotin-tyramine amide;N-(4-hydroxyphenethyl)biotinamide;TSA,Biotinyl tyramide,inhibit,Inhibitor;(3aS,4S,6aR)-hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H...;N-(4-Hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide;(3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide;1H-Thieno[3,4-d]iMidazole-4-pentanaMide, hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-, (3aS,4S,6aR)-;5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-(4-hydroxyphenyl)ethyl]pentanamide
- CBNumber:
- CB52638943
- Molecular Formula:
- C18H25N3O3S
- Molecular Weight:
- 363.47
- MOL File:
- 41994-02-9.mol
- Modify Date:
- 2024/4/3 12:01:48
Boiling point | 735.8±60.0 °C(Predicted) |
---|---|
Density | 1.227±0.06 g/cm3(Predicted) |
storage temp. | 2-8°C |
solubility | Soluble in DMSO (up to 35 mg/ml) or in Ethanol (up to 20 mg/ml) |
form | solid |
pka | 10.01±0.15(Predicted) |
color | White |
Stability | Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month. |
InChIKey | VZWXNOBHWODXCW-ZOBUZTSGSA-N |
SMILES | C1(=O)N[C@]2([H])[C@H](CCCCC(NCCC3=CC=C(O)C=C3)=O)SC[C@]2([H])N1 |
SAFETY
Risk and Safety Statements
Symbol(GHS) | GHS07 |
---|---|
Signal word | Warning |
Hazard statements | H302-H315-H319-H335 |
Precautionary statements | P261-P305+P351+P338 |
Biotinyl tyramide Chemical Properties,Uses,Production
Description
Biotinyl tyramide is a reagent that has been used for tyramide signal amplification (TSA) via catalyzed reporter deposition (CARD). In CARD, a reporter enzyme, such as horseradish peroxidase (HRP) conjugated to a secondary antibody, is bound to the target of interest and catalyzes the covalent deposition of biotinyl tyramide to the sample. The sample is then probed by a detector, such as streptavidin-HRP, allowing detection via chromogenic or fluorescent methods. Biotinyl tyramide has been used in immunohistochemistry, ELISA, Western blot, and in situ hybridization applications.
Uses
Biotinyl Tyramide is a Biotin (B389040) derivative, a growth factor present in minute amounts in every living cell. Plays an indispensable role in numerous naturally occurring carboxylation reactions. Occurs mainly bound to proteins or polypeptides. The richest sources are liver, kidney, pancreas, yeast, and milk. The biotin content of cancerous tumors is higher than that of normal tissue.
Solubility in water
Biotinyl tyramide is supplied as a solid. A stock solution may be made by dissolving the biotin tyramide in the solvent of choice, which should be purged with an inert gas. It is soluble in organic solvents such as DMS0 and dimethyl formamide. The solubility of biotinyl tyramide in these solvents is approximately 30 mg/ml.
This product is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, biotinyl tyramide should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. Biotinyl tyramide has a solubility of approximately 0.16 mg/ml in a 1:5 solution of DMSO: PBS(pH 7.2) using this method.
Biotinyl tyramide Preparation Products And Raw materials
Raw materials
Preparation Products
Biotinyl tyramide Suppliers
Supplier | Tel | Country | ProdList | Advantage | Inquiry |
---|---|---|---|---|---|
Xian confluore Biological Technology Co., Ltd. | +86-15680926068 +86-15680926068 | China | 2996 | 58 | Inquiry |
TargetMol Chemicals Inc. | +1-781-999-5354 +1-00000000000 | United States | 19892 | 58 | Inquiry |
Career Henan Chemica Co | +86-0371-86658258 +8613203830695 | China | 30250 | 58 | Inquiry |
Hefei TNJ Chemical Industry Co.,Ltd. | +86-0551-65418671 +8618949823763 | China | 34571 | 58 | Inquiry |
Labnetwork lnc. | +86-27-50766799 +8618062016861 | China | 19994 | 58 | Inquiry |
Alfa Chemistry | United States | 24072 | 58 | Inquiry | |
LEAPCHEM CO., LTD. | +86-852-30606658 | China | 43348 | 58 | Inquiry |
Jilin Chinese Academy of Sciences-yanshen Technology | +undefined18143011203 | China | 42057 | 58 | Inquiry |
Aladdin Scientific | +1-+1(833)-552-7181 | United States | 57511 | 58 | Inquiry |
VladaChem GmbH | +49-7246-3082843 | Germany | 1860 | 58 | Inquiry |
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