Trypan Blue
- CAS No.
- 72-57-1
- Chemical Name:
- Trypan Blue
- Synonyms
- trypan;blue3b;blueemb;parkipan;CI 23850;congoblue;ncic61289;parkibleu;TRUE BLUE;benzoblue
- CBNumber:
- CB8315001
- Molecular Formula:
- C34H24N6Na4O14S4
- Molecular Weight:
- 960.81
- MOL File:
- 72-57-1.mol
- Modify Date:
- 2024/3/8 14:38:52
| Melting point | >300 °C (lit.) |
|---|---|
| Density | 1.007 g/mL at 20 °C |
| storage temp. | dry at room temperature |
| solubility | H2O: soluble10mg/mL |
| Colour Index | 23850 |
| form | Powder |
| color | Dark greenish-brown |
| PH | 9.8 (10g/l, H2O, 20℃) |
| Water Solubility | 10 g/L (25 ºC) |
| Merck | 14,9792 |
| BRN | 4360496 |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| CAS DataBase Reference | 72-57-1(CAS DataBase Reference) |
| IARC | 2B (Vol. 8, Sup 7) 1987 |
| EPA Substance Registry System | Trypan blue (72-57-1) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS08 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Danger | |||||||||
| Hazard statements | H350 | |||||||||
| Precautionary statements | P201-P202-P280-P308+P313-P405-P501 | |||||||||
| Hazard Codes | T,Xi | |||||||||
| Risk Statements | 45-41-37/38-36/37/38-22 | |||||||||
| Safety Statements | 53-45-36/37/39-36-26 | |||||||||
| RIDADR | 2811 | |||||||||
| WGK Germany | 3 | |||||||||
| RTECS | QJ6475000 | |||||||||
| TSCA | Yes | |||||||||
| HS Code | 32041400 | |||||||||
| Toxicity | LD100 i.v. in rats: 300 mg/kg (Anderson) | |||||||||
| NFPA 704 |
|
Trypan Blue price More Price(16)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich(India) | T8154 | Trypan Blue solution 0.4%, liquid, sterile-filtered, suitable for cell culture | 72-57-1 | 20ML | ₹1498.5 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | T6146 | Trypan Blue powder, BioReagent, suitable for cell culture | 72-57-1 | 5G | ₹2009.1 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | T8154 | Trypan Blue solution 0.4%, liquid, sterile-filtered, suitable for cell culture | 72-57-1 | 100ML | ₹2419.8 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | T6146 | Trypan Blue powder, BioReagent, suitable for cell culture | 72-57-1 | 25G | ₹3607.5 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | T6146 | Trypan Blue powder, BioReagent, suitable for cell culture | 72-57-1 | 100G | ₹10567.2 | 2022-06-14 | Buy |
Trypan Blue Chemical Properties,Uses,Production
Chemical Properties
Trypan Blue is a dark Blue crystalline solid or powder that dissolves in water to form a blue solution. It's slightly soluble in soluble fiber yarn and doesn't dissolve in other organic solvents. When mixed with concentrated sulfuric acid, it turns dark greenish-blue, and when diluted, it becomes reddish-blue. With concentrated nitric acid, it creates a brownish-gray solution. In a 10% sulfuric acid solution, its aqueous solution turns red, while with sodium hydroxide, it becomes reddish-purple.
Preparation
3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-2,7-disulfonic acid(2 Moore) coupled.
Definition
ChEBI: Trypan blue is an organosulfonate salt that is the tetrasodium salt of 3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonic acid). It has a role as a histological dye, a fluorochrome and a carcinogenic agent. It is an organosulfonate salt and an organic sodium salt. It contains a trypan blue(4-).
General Description
Bluish-gray to dark blue powder.
Air & Water Reactions
Azo dyes can be explosive when suspended in air at specific concentrations. Slightly soluble in water.
Reactivity Profile
Direct Blue 14 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 14 is incompatible with strong oxidizing agents and strong acids.
Health Hazard
ACUTE/CHRONIC HAZARDS: Direct Blue 14 may cause irritation and may be absorbed through the skin. It is a positive animal carcinogen. When heated to decomposition Direct Blue 14 emits very toxic fumes of nitrogen oxides, sulfur oxides, carbon monoxide and carbon dioxide.
Fire Hazard
Flash point data for Direct Blue 14 are not available; however, Direct Blue 14 is probably combustible.
Safety Profile
Suspected carcinogen with experimental carcinogenic, neoplastigenic, and tumorigenic data. Poison by intraperitoneal, intravenous, and subcutaneous routes. Experimental teratogenic and reproductive effects. Mutation data reported. When heated to decomposition it emits very toxic fumes of NOx NazO, and SOx
Potential Exposure
Used in dyeing textiles; leather and paper; as a biological stain.
Shipping
UN2811 Toxic solids, organic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required. UN3143 dyes, solid, toxic, n.o.s. or dye intermediates, solid, toxic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required.
Incompatibilities
Dust may form explosive mixture with air. Trypan Blue is incompatible with strong oxidizing agents. Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides. Azo compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acylhalides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combinations can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Trypan Blue is sensitive to prolonged exposure to heat.
Waste Disposal
Consult with environmental regulatory agencies for guidance on acceptable disposal practices. Generators of waste containing this contaminant (≥100 kg/mo) must conform with EPA regulations governing storage, transportation, treatment, and waste disposal.
Trypan Blue Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | Inquiry |
|---|---|---|---|---|---|
| Arnish Laborates Pvt Ltd | +91 7208475595 | Maharashtra, India | 187 | 58 | Inquiry |
| JSK Chemicals | +919879767970 | Gujarat, India | 3756 | 58 | Inquiry |
| Esjay Chemicals | +919820452929 | Maharashtra, India | 110 | 58 | Inquiry |
| Tanya Exports | +91-9825040588 +91-9825033002 | Gujarat, India | 186 | 58 | Inquiry |
| Appex Dye Stuff Industries | +91-9974922439 +91-9974922439 | Gujarat, India | 146 | 58 | Inquiry |
| GSP Dyes and Chemicals Pvt.Ltd | +91-22-28144802 +91-2228144802 | Maharashtra, India | 79 | 58 | Inquiry |
| Anubhav Business Corporation | +91-2228014519 +91-2228014999 | Maharashtra, India | 119 | 58 | Inquiry |
| Emichem Pvt Ltd | +91-3340440048 +91-9831626921 | West Bengal, India | 176 | 58 | Inquiry |
| Hariram Industries | +91-9913303151 +91-9427102885 | Gujarat, India | 59 | 58 | Inquiry |
| Macsen Labs | +91-7296866111 +91-9309241616 | Rajasthan, India | 11 | 58 | Inquiry |
| Supplier | Advantage |
|---|---|
| Arnish Laborates Pvt Ltd | 58 |
| JSK Chemicals | 58 |
| Esjay Chemicals | 58 |
| Tanya Exports | 58 |
| Appex Dye Stuff Industries | 58 |
| GSP Dyes and Chemicals Pvt.Ltd | 58 |
| Anubhav Business Corporation | 58 |
| Emichem Pvt Ltd | 58 |
| Hariram Industries | 58 |
| Macsen Labs | 58 |
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