Ethidium bromide manufacturers and suppliers in india

Ethidium bromide

Properties

Melting point:: 260-262 °C (dec.)(lit.)

Density: 1.3739 (rough estimate)

refractive index: 1.6700 (estimate)

Flash point:: >100°C

storage temp.: 2-8°C

solubility: H₂O: 10 mg/mL, opaque, strongly red

form: powder

color: Red to dark purple

Odor: Odorless solid

Water Solubility: 40 g/L (25 ºC)

λmax: 518 nm, 210 nm, 285 nm, 316 nm, 343 nm, 480 nm, 525 nm

Merck: 14,4731

BRN: 3642536

Stability:: Stable. Incompatible with strong oxidizing agents.

Biological Applications: Nucleic acid hybridization; detecting nucleic acids,cells,cancer cells,human cytomegalovirus,hydrogenase A (hydA) of Clostridia,influenza A virus,oligonucleotides,viable Plesiomonas shigelloides; apoptosis assay; nucleic acid quantification

Major Application: Electroluminescent displays;photoresists

Safety Information

Symbol(GHS): GHS06,GHS08

Signal word: Danger

Hazard statements: H331-H341

Precautionary statements: P202-P261-P271-P280-P304+P340+P311-P308+P313

Hazard Codes: T,T+

Risk Statements: 23-68-36/37/38-26-21/22-22

Safety Statements: 36/37-45-36/37/39-28A-26-22-28-63

RIDADR: UN 2811 6.1/PG 1

WGK Germany: 3

RTECS: SF7950000

F: 8-9

HazardClass: 6.1

PackingGroup: I

HS Code: 29339990

Toxicity: Intercalating dye widely used to stain DNA in gels and gradients. The DNA can be visualized readily by irradiation with ultraviolet light, as little as 0.5 μg of DNA being detectable by such methods. Ethidium bromide is also added to cesium chloride density gradients, since as an intercalating molecule it binds more readily to linear DNA than to closed collinear circles of DNA (such as plasmids). Binding of ethidium bromide reduces the density of DNA, thus covalent circles of DNA have higher densities at saturating concentrations of ethidium bromide, permitting the separation of plasmid DNA. Ethidium bromide is a putative carcinogen.

Use

Ethidium bromide (EtBr) is a fluorescent dye widely used in molecular biology research. Early usage was as a veterinary trypanocide. It is a mutagenic compound that intercalates double-stranded DNA and RNA. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA, 25-fold on binding double-stranded DNA (although histones block binding of EtBr to DNA). Ethidium bromide has been used in multiple fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of the binding to DNA, EtBr is a powerful inhibitor of DNA polymerase.