Ethidium bromide
- CAS No.
- 1239-45-8
- Chemical Name:
- Ethidium bromide
- Synonyms
- ETBR;Ethidium bromide solution;HOMIDIUM BROMIDE;Rd1572;Dromilac;Homidium;-Cellstain-Eb;Ethidium Brmide;ETHIDIUM BROMIDE;EthidiumBromideGr
- CBNumber:
- CB7406991
- Molecular Formula:
- C21H20BrN3
- Molecular Weight:
- 394.32
- MOL File:
- 1239-45-8.mol
- MSDS File:
- SDS
- Modify Date:
- 2024/8/8 18:06:34
| Melting point | 260-262 °C (dec.)(lit.) |
|---|---|
| Density | 1.3739 (rough estimate) |
| refractive index | 1.6700 (estimate) |
| Flash point | >100°C |
| storage temp. | 2-8°C |
| solubility | H2O: 10 mg/mL, opaque, strongly red |
| form | powder |
| color | Red to dark purple |
| Odor | Odorless solid |
| Water Solubility | 40 g/L (25 ºC) |
| λmax | 518 nm, 210 nm, 285 nm, 316 nm, 343 nm, 480 nm, 525 nm |
| Merck | 14,4731 |
| BRN | 3642536 |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| Biological Applications | Nucleic acid hybridization; detecting nucleic acids,cells,cancer cells,human cytomegalovirus,hydrogenase A (hydA) of Clostridia,influenza A virus,oligonucleotides,viable Plesiomonas shigelloides; apoptosis assay; nucleic acid quantification |
| Major Application | Electroluminescent displays;photoresists |
| CAS DataBase Reference | 1239-45-8(CAS DataBase Reference) |
| EPA Substance Registry System | Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1239-45-8) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |   GHS06,GHS08 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Danger | |||||||||
| Hazard statements | H331-H341 | |||||||||
| Precautionary statements | P202-P261-P271-P280-P304+P340+P311-P308+P313 | |||||||||
| Hazard Codes | T,T+ | |||||||||
| Risk Statements | 23-68-36/37/38-26-21/22-22 | |||||||||
| Safety Statements | 36/37-45-36/37/39-28A-26-22-28-63 | |||||||||
| RIDADR | UN 2811 6.1/PG 1 | |||||||||
| WGK Germany | 3 | |||||||||
| RTECS | SF7950000 | |||||||||
| F | 8-9 | |||||||||
| HazardClass | 6.1 | |||||||||
| PackingGroup | I | |||||||||
| HS Code | 29339990 | |||||||||
| Toxicity | Intercalating dye widely used to stain DNA in gels and gradients. The DNA can be visualized readily by irradiation with ultraviolet light, as little as 0.5 μg of DNA being detectable by such methods. Ethidium bromide is also added to cesium chloride density gradients, since as an intercalating molecule it binds more readily to linear DNA than to closed collinear circles of DNA (such as plasmids). Binding of ethidium bromide reduces the density of DNA, thus covalent circles of DNA have higher densities at saturating concentrations of ethidium bromide, permitting the separation of plasmid DNA. Ethidium bromide is a putative carcinogen. | |||||||||
| NFPA 704 |
|
Ethidium bromide price More Price(27)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich(India) | E8751 | Ethidium bromide ~95% (HPLC) | 1239-45-8 | 1G | ₹4568.15 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | E8751 | Ethidium bromide ~95% (HPLC) | 1239-45-8 | 5G | ₹10045.6 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | E8751 | Ethidium bromide ~95% (HPLC) | 1239-45-8 | 10G | ₹22537.65 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | E8751 | Ethidium bromide ~95% (HPLC) | 1239-45-8 | 25G | ₹43310.83 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | E7637 | Ethidium bromide BioReagent, for molecular biology, powder | 1239-45-8 | 1G | ₹5217.65 | 2022-06-14 | Buy |
Ethidium bromide Chemical Properties,Uses,Production
Chemical Properties
Purple/maroon crystalline powder
Uses
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma).
Health Hazard
Acute toxic effects from exposure to ethidium bromide have not been thoroughly investigated. Ethidium bromide is irritating to the eyes, skin, mucous membranes, and upper respiratory tract. Although there is no evidence for the carcinogenicity or teratogenicity of this substance in humans, ethidium bromide is strongly mutagenic and therefore should be regarded as a possible carcinogen and reproductive toxin
Flammability and Explosibility
Ethidium bromide does not pose a flammability hazard (NFPA rating = 1).
Safety Profile
Because ethidium bromide can bind with DNA, it is highly toxic as a mutagen. It may potentially cause carcinogenic or teratogenic effects, although no scientific evidence showing either health effect has been found. Exposure routes of ethidium bromide are inhalation, ingestion, and skin absorption. An acute exposure to ethidium bromide causes irritation of the mouth, upper respiratory tract, skin, and eyes.
storage
Because of its mutagenicity, stock solutions of this compound should be prepared in a fume hood, and protective gloves should be worn at all times while handling this substance. Operations capable of generating ethidium bromide dust or aerosols of ethidium bromide solutions should be conducted in a fume hood to prevent exposure by inhalation.
Purification Methods
Crystallise it from MeOH or EtOH [Lamos et al. J Am Chem Soc 108 4278 1986]. Its solubility in H2O is 1%. [Beilstein 22/11 V 352.] POSSIBLE CARCINOGEN.
Incompatibilities
No incompatibilities are known.
Waste Disposal
Excess ethidium bromide and waste material containing this substance should be placed in an appropriate container, clearly labeled, and handled according to your institution's waste disposal guidelines.
Ethidium bromide Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | Inquiry |
|---|---|---|---|---|---|
| GLR Innovations | +91 9891111994 | New Delhi, India | 4542 | 58 | Inquiry |
| GRINDLAYS PHARMACEUTICALS PRIVATE LIMITED | +91-91-22-25278801 +91-2225278801 | Mumbai, India | 83 | 58 | Inquiry |
| Evans Fine Chem | +91-9821340302 +91-9821340302 | Maharashtra, India | 286 | 58 | Inquiry |
| JSK Chemicals | +919879767970 | Gujarat, India | 3756 | 58 | Inquiry |
| Dhara Industries | +91-9322395199 +91-9322395199 | Mumbai, India | 190 | 58 | Inquiry |
| Shiv Shakti Pharma Chem | +91-8291900674 +91-9594999721 | Maharashtra, India | 7 | 58 | Inquiry |
| CHEMSWORTH | +91-261-2397244 | New Delhi, India | 6707 | 30 | Inquiry |
| OCEAN TRADING CORPORATION | +91(22) 24921669 | New Delhi, India | 6211 | 58 | Inquiry |
| Scientific OEM | +91-22- 2343 7546 / 2341 3094 | New Delhi, India | 1996 | 38 | Inquiry |
| Otto Chemie Pvt. Ltd. | +91 9820041841 | Mumbai, India | 5873 | 58 | Inquiry |
| Supplier | Advantage |
|---|---|
| GLR Innovations | 58 |
| GRINDLAYS PHARMACEUTICALS PRIVATE LIMITED | 58 |
| Evans Fine Chem | 58 |
| JSK Chemicals | 58 |
| Dhara Industries | 58 |
| Shiv Shakti Pharma Chem | 58 |
| CHEMSWORTH | 30 |
| OCEAN TRADING CORPORATION | 58 |
| Scientific OEM | 38 |
| Otto Chemie Pvt. Ltd. | 58 |
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