Ethidium bromide
Ethidium bromide
- CAS:
- 1239-45-8
- MF:
- C21H20BrN3
- MW:
- 394.32
Suppliers by country/region
Company Type
Properties
- Melting point:
- 260-262 °C (dec.)(lit.)
- Density
- 1.3739 (rough estimate)
- refractive index
- 1.6700 (estimate)
- Flash point:
- >100°C
- storage temp.
- 2-8°C
- solubility
- H2O: 10 mg/mL, opaque, strongly red
- form
- powder
- color
- Red to dark purple
- Odor
- Odorless solid
- Water Solubility
- 40 g/L (25 ºC)
- λmax
- 518 nm, 210 nm, 285 nm, 316 nm, 343 nm, 480 nm, 525 nm
- Merck
- 14,4731
- BRN
- 3642536
- Stability:
- Stable. Incompatible with strong oxidizing agents.
- Biological Applications
- Nucleic acid hybridization; detecting nucleic acids,cells,cancer cells,human cytomegalovirus,hydrogenase A (hydA) of Clostridia,influenza A virus,oligonucleotides,viable Plesiomonas shigelloides; apoptosis assay; nucleic acid quantification
- Major Application
- Electroluminescent displays;photoresists
Safety Information
- Symbol(GHS)
GHS06,GHS08
- Signal word
- Danger
- Hazard statements
- H331-H341
- Precautionary statements
- P202-P261-P271-P280-P304+P340+P311-P308+P313
- Hazard Codes
- T,T+
- Risk Statements
- 23-68-36/37/38-26-21/22-22
- Safety Statements
- 36/37-45-36/37/39-28A-26-22-28-63
- RIDADR
- UN 2811 6.1/PG 1
- WGK Germany
- 3
- RTECS
- SF7950000
- F
- 8-9
- HazardClass
- 6.1
- PackingGroup
- I
- HS Code
- 29339990
- Toxicity
- Intercalating dye widely used to stain DNA in gels and gradients. The DNA can be visualized readily by irradiation with ultraviolet light, as little as 0.5 μg of DNA being detectable by such methods. Ethidium bromide is also added to cesium chloride density gradients, since as an intercalating molecule it binds more readily to linear DNA than to closed collinear circles of DNA (such as plasmids). Binding of ethidium bromide reduces the density of DNA, thus covalent circles of DNA have higher densities at saturating concentrations of ethidium bromide, permitting the separation of plasmid DNA. Ethidium bromide is a putative carcinogen.
Use
Ethidium bromide (EtBr) is a fluorescent dye widely used in molecular biology research. Early usage was as a veterinary trypanocide. It is a mutagenic compound that intercalates double-stranded DNA and RNA. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA, 25-fold on binding double-stranded DNA (although histones block binding of EtBr to DNA). Ethidium bromide has been used in multiple fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of the binding to DNA, EtBr is a powerful inhibitor of DNA polymerase.
21 India supplier list of "Ethidium bromide"
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- Product Name:Homidium bromide
- Products Intro:Purity: 99% | Package: 1 kg,5 kg, 10 kg,25kg and 1 MT
- Company Type: Manufacturer
- Country/Region: Mumbai,INDIA
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- Product Name:Ethidium bromide, 98%
- Products Intro:Purity: 99% | Package: 1 kg,5 kg, 10 kg,25kg and 1 MT
- Company Type: Trader
- Country/Region: Gujarat,INDIA
![](/images/pro-3.jpg)
- Product Name:Homidium bromide
- Products Intro:Purity: 98% | Package: 1 kg,5 kg, 10 kg,25kg and 1 MT
- Company Type: Manufacturer
- Country/Region: Maharashtra,INDIA
![](/images/pro-3.jpg)
- Product Name:Ethidium Bromide
- Products Intro:Purity: 98% | Package: 1 kg,5 kg, 10 kg,25kg and 1 MT
- Company Type: Manufacturer
- Country/Region: Mumbai,INDIA
![](/images/pro-3.jpg)
- Product Name:ETHIDIUM BROMIDE
- Products Intro:Purity: 99% | Package: 1 kg,5 kg, 10 kg,25kg and 1 MT
- Company Type: Manufacturer
- Country/Region: Maharashtra,INDIA
![](/images/pro-3.jpg)
- Product Name:Ethidiumbromidesolution
- Products Intro:Brand:sigma aldrich | Product Number:E1385 | Purity:BioReagent,formolecularbiology,500?μg/mLinH2O
- Company Type: Trader
- Country/Region: Bangalore,INDIA