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ChemicalBook > Product Catalogue >Analytical Chemistry >Analysis reagents >Ion chromatography reagents >HEPES

HEPES

HEPES Structure
  • ₹198 - ₹1025820.3
  • Product name: HEPES
  • CAS: 7365-45-9
  • MF: C8H18N2O4S
  • MW: 238.3
  • EINECS:230-907-9
  • MDL Number:MFCD00006158
  • Synonyms:HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID MOLECULAR BIOLOGY GRADE;HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 99+%;N-(2-HYDROXYETHYL)-PIPERAZINE-N'-2-ETHANESULFONIC ACID(4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID(HEPES-ACID));Hepes,FreeAcid(N-[2-Hydroxyethyl]Piperaz;1-HeptaneSulfonicAcidSod.SaltGr;N-(2-Hydroxyethyl)Piperazine-N'-(3-EthanesulfonicAcid);N-(2-Hydroxyethyl)Piperazine-N'-(2-EthanesulfonicAcid)(Hepes);Hepes,LowSodium,FreeAcid
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  • Sigma-Aldrich(India)
  • TCI Chemicals (India)
  • SRL
  • ottokemi

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  • ManufacturerSigma-Aldrich(India)
  • Product numberH0887
  • Product descriptionHEPES solution 1?M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
  • Packaging20ML
  • Price₹3334.1
  • Updated2022-06-14
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  • ManufacturerSigma-Aldrich(India)
  • Product number54457
  • Product descriptionHEPES BioUltra, for molecular biology, ≥99.5% (T)
  • Packaging10G
  • Price₹4459.9
  • Updated2022-06-14
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  • ManufacturerSigma-Aldrich(India)
  • Product number51558
  • Product descriptionHEPES buffered saline BioUltra, for molecular biology, 2× concentrate
  • Packaging50ML
  • Price₹6051.5
  • Updated2022-06-14
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  • ManufacturerSigma-Aldrich(India)
  • Product numberH6147
  • Product descriptionHEPES BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)
  • Packaging25G
  • Price₹6765.63
  • Updated2022-06-14
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  • ManufacturerSigma-Aldrich(India)
  • Product numberH3375
  • Product descriptionHEPES ≥99.5% (titration)
  • Packaging25G
  • Price₹6776.45
  • Updated2022-06-14
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  • ManufacturerSigma-Aldrich(India)
  • Product number83264
  • Product descriptionHEPES buffer solution 1?M in H2O
  • Packaging100ML
  • Price₹7014.6
  • Updated2022-06-14
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  • ManufacturerSigma-Aldrich(India)
  • Product numberH3375
  • Product descriptionHEPES ≥99.5% (titration)
  • Packaging25KG
  • Price₹525726.95
  • Updated2022-06-14
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  • ManufacturerSigma-Aldrich(India)
  • Product numberH4034
  • Product descriptionHEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
  • Packaging25KG
  • Price₹538543.75
  • Updated2022-06-14
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  • ManufacturerSigma-Aldrich(India)
  • Product numberH3375
  • Product descriptionHEPES ≥99.5% (titration)
  • Packaging50KG
  • Price₹1025820.3
  • Updated2022-06-14
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  • ManufacturerSigma-Aldrich(India)
  • Product numberRDD002
  • Product descriptionHEPES anhydrous, free-flowing, Redi-Dri?, ≥99.5%
  • Packaging1KG
  • Price₹65361.35
  • Updated2022-06-14
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  • ManufacturerSigma-Aldrich(India)
  • Product numberH3375
  • Product descriptionHEPES ≥99.5% (titration)
  • Packaging1KG
  • Price₹74421.88
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberH4034
  • Product descriptionHEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
  • Packaging1KG
  • Price₹77658.55
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberRDD002
  • Product descriptionHEPES anhydrous, free-flowing, Redi-Dri?, ≥99.5%
  • Packaging2.5KG
  • Price₹107427.3
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberH0887
  • Product descriptionHEPES solution 1?M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
  • Packaging20X100ML
  • Price₹115156.35
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberH3375
  • Product descriptionHEPES ≥99.5% (titration)
  • Packaging5KG
  • Price₹211888.55
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberH7523
  • Product descriptionHEPES BioXtra, pH 5.0-6.5 (1?M in H2O), ≥99.5% (titration)
  • Packaging250G
  • Price₹35570.95
  • Updated2022-06-14
  • Buy
Manufacturer Product number Product description Packaging Price Updated Buy
Sigma-Aldrich(India) H0887 HEPES solution 1?M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture 20ML ₹3334.1 2022-06-14 Buy
Sigma-Aldrich(India) 54457 HEPES BioUltra, for molecular biology, ≥99.5% (T) 10G ₹4459.9 2022-06-14 Buy
Sigma-Aldrich(India) 51558 HEPES buffered saline BioUltra, for molecular biology, 2× concentrate 50ML ₹6051.5 2022-06-14 Buy
Sigma-Aldrich(India) H6147 HEPES BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration) 25G ₹6765.63 2022-06-14 Buy
Sigma-Aldrich(India) H3375 HEPES ≥99.5% (titration) 25G ₹6776.45 2022-06-14 Buy
Sigma-Aldrich(India) 83264 HEPES buffer solution 1?M in H2O 100ML ₹7014.6 2022-06-14 Buy
Sigma-Aldrich(India) H3375 HEPES ≥99.5% (titration) 25KG ₹525726.95 2022-06-14 Buy
Sigma-Aldrich(India) H4034 HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture 25KG ₹538543.75 2022-06-14 Buy
Sigma-Aldrich(India) H3375 HEPES ≥99.5% (titration) 50KG ₹1025820.3 2022-06-14 Buy
Sigma-Aldrich(India) RDD002 HEPES anhydrous, free-flowing, Redi-Dri?, ≥99.5% 1KG ₹65361.35 2022-06-14 Buy
Sigma-Aldrich(India) H3375 HEPES ≥99.5% (titration) 1KG ₹74421.88 2022-06-14 Buy
Sigma-Aldrich(India) H4034 HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture 1KG ₹77658.55 2022-06-14 Buy
Sigma-Aldrich(India) RDD002 HEPES anhydrous, free-flowing, Redi-Dri?, ≥99.5% 2.5KG ₹107427.3 2022-06-14 Buy
Sigma-Aldrich(India) H0887 HEPES solution 1?M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture 20X100ML ₹115156.35 2022-06-14 Buy
Sigma-Aldrich(India) H3375 HEPES ≥99.5% (titration) 5KG ₹211888.55 2022-06-14 Buy
Sigma-Aldrich(India) H7523 HEPES BioXtra, pH 5.0-6.5 (1?M in H2O), ≥99.5% (titration) 250G ₹35570.95 2022-06-14 Buy

Properties

Melting point :234-238 °C
Boiling point :408℃[at 101 325 Pa]
Density :1.07 g/mL at 20 °C
vapor pressure :0Pa at 25℃
refractive index :n20/D 1.339
storage temp. :2-8°C
solubility :H2O: 1 M at 20 °C, clear, colorless
form :powder
color :White
PH :5.0-6.5 (1Maqueous solution)
pka :7.5(at 25℃)
PH Range :7.0 - 7.6
Water Solubility :Soluble
λmax :λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04 Merck :14,4654
BRN :883043
Stability :Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
InChIKey :JKMHFZQWWAIEOD-UHFFFAOYSA-N
LogP :-3.85 at 20℃
CAS DataBase Reference :7365-45-9(CAS DataBase Reference)
EPA Substance Registry System :1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9)

Safety Information

Symbol(GHS): GHS hazard pictograms
Signal word: Warning
Hazard statements:
Code Hazard statements Hazard class Category Signal word Pictogram P-Codes
H302 Harmful if swallowed Acute toxicity,oral Category 4 Warning GHS hazard pictograms P264, P270, P301+P312, P330, P501
H315 Causes skin irritation Skin corrosion/irritation Category 2 Warning GHS hazard pictograms P264, P280, P302+P352, P321,P332+P313, P362
H319 Causes serious eye irritation Serious eye damage/eye irritation Category 2A Warning GHS hazard pictograms P264, P280, P305+P351+P338,P337+P313P
H335 May cause respiratory irritation Specific target organ toxicity, single exposure;Respiratory tract irritation Category 3 Warning GHS hazard pictograms
Precautionary statements:
P261 Avoid breathing dust/fume/gas/mist/vapours/spray.
P271 Use only outdoors or in a well-ventilated area.
P280 Wear protective gloves/protective clothing/eye protection/face protection.

Description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

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