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ChemicalBook > Product Catalogue >Chemical Reagents >Organic reagents >Aromatics >Tetramethylbenzidine

Tetramethylbenzidine

Tetramethylbenzidine Structure
  • ₹610 - ₹331342.43
  • Product name: Tetramethylbenzidine
  • CAS: 54827-17-7
  • MF: C16H20N2
  • MW: 240.34
  • EINECS:259-364-6
  • MDL Number:MFCD00007748
  • Synonyms:3,3',5,5'-TETRAMETHYLBENZIDINE LIQUID SUBSTRATE SYSTEM;3,3',5,5'-TETRAMETHYLBENZIDINE SUBSTRATE;3,3',5,5'-TETRAMETHYLBENZIDIN;3,3',5',5-TETRAMETHYLBENZIDINE;3,3',5,5'-TETRAMETHYLBENZIDINE;3,3'-5,5-TETRAMETHYLBENZIDINE;3,3',5,5'-Tetramethylbenzidine [for Biochemical Research];3,3',5,5'-Tetramethylbiphenyl-4,4'-diamine
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  • Sigma-Aldrich(India)
  • ALFA India
  • TCI Chemicals (India)
  • SRL
  • ottokemi

Package

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  • 100ML
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  • 50TABLETS
  • 100TABLETS
  • ManufacturerSigma-Aldrich(India)
  • Product number860336
  • Product description3,3′,5,5′-Tetramethylbenzidine ≥99%
  • Packaging100MG
  • Price₹1948.5
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberT2885
  • Product description3,3′,5,5′-Tetramethylbenzidine ≥98% (TLC)
  • Packaging100MG
  • Price₹3377.4
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product number09743
  • Product description3,3′,5,5′-Tetramethylbenzidine standard for GC
  • Packaging250MG
  • Price₹3464
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberT2885
  • Product description3,3′,5,5′-Tetramethylbenzidine ≥98% (TLC)
  • Packaging1G
  • Price₹4351.65
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product number860336
  • Product description3,3′,5,5′-Tetramethylbenzidine ≥99%
  • Packaging1G
  • Price₹7003.78
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product number87748
  • Product description3,3′,5,5′-Tetramethylbenzidine ≥98.0% (NT)
  • Packaging1G
  • Price₹7707.4
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberT2885
  • Product description3,3′,5,5′-Tetramethylbenzidine ≥98% (TLC)
  • Packaging100G
  • Price₹331342.43
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product number860336
  • Product description3,3′,5,5′-Tetramethylbenzidine ≥99%
  • Packaging5G
  • Price₹23436.13
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product number87748
  • Product description3,3′,5,5′-Tetramethylbenzidine ≥98.0% (NT)
  • Packaging5G
  • Price₹25828.45
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberT5525
  • Product description3,3′,5,5′-Tetramethylbenzidine tablet, 1 mg substrate per tablet
  • Packaging100TABLETS
  • Price₹28209.3
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product number860336
  • Product description3,3′,5,5′-Tetramethylbenzidine ≥99%
  • Packaging25G
  • Price₹47207.83
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product number87748
  • Product description3,3′,5,5′-Tetramethylbenzidine ≥98.0% (NT)
  • Packaging25G
  • Price₹50206.35
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberCL07
  • Product descriptionTMB Solution This TMB Solution is validated for use in ELISA for the detection of TMB Solution.
  • Packaging1000ML
  • Price₹56969.99
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product number1.08622
  • Product description3,3′,5,5′-Tetramethylbenzidine GR for analysis
  • Packaging1G
  • Price₹9800
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product number613544
  • Product descriptionTMB, Soluble
  • Packaging100ML
  • Price₹10600
  • Updated2022-06-14
  • Buy
  • ManufacturerSigma-Aldrich(India)
  • Product numberCL07
  • Product descriptionTMB Solution This TMB Solution is validated for use in ELISA for the detection of TMB Solution.
  • Packaging100ML
  • Price₹11250.01
  • Updated2022-06-14
  • Buy
Manufacturer Product number Product description Packaging Price Updated Buy
Sigma-Aldrich(India) 860336 3,3′,5,5′-Tetramethylbenzidine ≥99% 100MG ₹1948.5 2022-06-14 Buy
Sigma-Aldrich(India) T2885 3,3′,5,5′-Tetramethylbenzidine ≥98% (TLC) 100MG ₹3377.4 2022-06-14 Buy
Sigma-Aldrich(India) 09743 3,3′,5,5′-Tetramethylbenzidine standard for GC 250MG ₹3464 2022-06-14 Buy
Sigma-Aldrich(India) T2885 3,3′,5,5′-Tetramethylbenzidine ≥98% (TLC) 1G ₹4351.65 2022-06-14 Buy
Sigma-Aldrich(India) 860336 3,3′,5,5′-Tetramethylbenzidine ≥99% 1G ₹7003.78 2022-06-14 Buy
Sigma-Aldrich(India) 87748 3,3′,5,5′-Tetramethylbenzidine ≥98.0% (NT) 1G ₹7707.4 2022-06-14 Buy
Sigma-Aldrich(India) T2885 3,3′,5,5′-Tetramethylbenzidine ≥98% (TLC) 100G ₹331342.43 2022-06-14 Buy
Sigma-Aldrich(India) 860336 3,3′,5,5′-Tetramethylbenzidine ≥99% 5G ₹23436.13 2022-06-14 Buy
Sigma-Aldrich(India) 87748 3,3′,5,5′-Tetramethylbenzidine ≥98.0% (NT) 5G ₹25828.45 2022-06-14 Buy
Sigma-Aldrich(India) T5525 3,3′,5,5′-Tetramethylbenzidine tablet, 1 mg substrate per tablet 100TABLETS ₹28209.3 2022-06-14 Buy
Sigma-Aldrich(India) 860336 3,3′,5,5′-Tetramethylbenzidine ≥99% 25G ₹47207.83 2022-06-14 Buy
Sigma-Aldrich(India) 87748 3,3′,5,5′-Tetramethylbenzidine ≥98.0% (NT) 25G ₹50206.35 2022-06-14 Buy
Sigma-Aldrich(India) CL07 TMB Solution This TMB Solution is validated for use in ELISA for the detection of TMB Solution. 1000ML ₹56969.99 2022-06-14 Buy
Sigma-Aldrich(India) 1.08622 3,3′,5,5′-Tetramethylbenzidine GR for analysis 1G ₹9800 2022-06-14 Buy
Sigma-Aldrich(India) 613544 TMB, Soluble 100ML ₹10600 2022-06-14 Buy
Sigma-Aldrich(India) CL07 TMB Solution This TMB Solution is validated for use in ELISA for the detection of TMB Solution. 100ML ₹11250.01 2022-06-14 Buy

Properties

Melting point :168-171 °C(lit.)
Boiling point :100 °C
Density :1
refractive index :1.5519 (estimate)
storage temp. :2-8°C
solubility :Slightly soluble. <0.1 g/100 mL at 20°C.
pka :4.49±0.10(Predicted)
form :tablet
color :Cream
Water Solubility :Slightly soluble. <0.1 g/100 mL at 20 ºC
Sensitive :Light Sensitive
BRN :2808541
Stability :Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents.
CAS DataBase Reference :54827-17-7(CAS DataBase Reference)
EPA Substance Registry System :3,3',5,5'-Tetramethylbenzidine (54827-17-7)

Safety Information

Symbol(GHS): GHS hazard pictogramsGHS hazard pictogramsGHS hazard pictograms
Signal word: Warning
Hazard statements:
Code Hazard statements Hazard class Category Signal word Pictogram P-Codes
H225 Highly Flammable liquid and vapour Flammable liquids Category 2 Danger GHS hazard pictograms P210,P233, P240, P241, P242, P243,P280, P303+ P361+P353, P370+P378,P403+P235, P501
H302 Harmful if swallowed Acute toxicity,oral Category 4 Warning GHS hazard pictograms P264, P270, P301+P312, P330, P501
H312 Harmful in contact with skin Acute toxicity,dermal Category 4 Warning GHS hazard pictograms P280,P302+P352, P312, P322, P363,P501
H315 Causes skin irritation Skin corrosion/irritation Category 2 Warning GHS hazard pictograms P264, P280, P302+P352, P321,P332+P313, P362
H319 Causes serious eye irritation Serious eye damage/eye irritation Category 2A Warning GHS hazard pictograms P264, P280, P305+P351+P338,P337+P313P
H332 Harmful if inhaled Acute toxicity,inhalation Category 4 Warning GHS hazard pictograms P261, P271, P304+P340, P312
H335 May cause respiratory irritation Specific target organ toxicity, single exposure;Respiratory tract irritation Category 3 Warning GHS hazard pictograms
H370 Causes damage to organs Specific target organ toxicity, single exposure Category 1 Danger GHS hazard pictograms P260, P264, P270, P307+P311, P321,P405, P501
Precautionary statements:
P210 Keep away from heat/sparks/open flames/hot surfaces. — No smoking.
P260 Do not breathe dust/fume/gas/mist/vapours/spray.
P261 Avoid breathing dust/fume/gas/mist/vapours/spray.
P280 Wear protective gloves/protective clothing/eye protection/face protection.
P303+P361+P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P304+P340 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P405 Store locked up.

Description

Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). It is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. Tetramethylbenzidine is degraded by sunlight and by fluorescent lights. It is a soluble chromogen substrate for horseradish peroxidase detection systems. Tetramethylbenzidine is recommended for ELISA procedures.

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