3,4-Dihydroxyphenylacetic acid synthesis
- Product Name:3,4-Dihydroxyphenylacetic acid
- CAS Number:102-32-9
- Molecular formula:C8H8O4
- Molecular Weight:168.15
156-38-7
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102-32-9
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$20.00/1g
Yield:102-32-9 99.7%
Reaction Conditions:
with ascorbic acid in aq. phosphate buffer at 25;
Steps:
2.5. Reaction to produce 3,4-dihydroxyphenylacetic acid (DHPAA) from 4-hydroxyphenylacetic acid (HPAA)
A typical reaction was started by addition of 15.0 mg of tyrosinaseCLEAs into 20.0 mL of Na phosphate buffer (50 mM, pH 6.0) containing10.0mM HPAA and 15.0mM L-ascorbic acid in a 50 mL conical flask,which was placed in a shaking incubator with agitation of 200 rpm at25 °C. Periodically, a 0.15 mL sample was taken which was then fourtimes diluted with the buffer before subjected to HPLC analysis as describedbelow.
References:
Cheng, Hui;Zou, Yong;Luo, Xiang;Song, Xian-Heng;Yang, Zhen [Process Biochemistry,2018,vol. 70,p. 90 - 97]
93-40-3
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102-32-9
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1126-62-1
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102-32-9
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103-82-2
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621-37-4
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102-32-9
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$20.00/1g
451-13-8
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$24.00/25mg