Iodonitrotetrazolium chloride
- CAS No.
- 146-68-9
- Chemical Name:
- Iodonitrotetrazolium chloride
- Synonyms
- INT;INT DYE;IntA.R.;INT CHLORIDE;2-(p-iodophe;IODONITROTETRAZOLIUM;IODONITROTETRAZOLIUM VIOLET;Iodonitrotetrazolium purple;IODONITRO TETRAZOLIUM (INT);odonitrotetrazolium chloride
- CBNumber:
- CB7232772
- Molecular Formula:
- C19H13ClIN5O2
- Molecular Weight:
- 505.7
- MOL File:
- 146-68-9.mol
- MSDS File:
- SDS
- Modify Date:
- 2024/3/19 13:11:16
| Melting point | 240 °C (dec.)(lit.) |
|---|---|
| storage temp. | 2-8°C |
| solubility | methanol: water (1:1): 50 mg/mL hot, very faintly turbid, very deep yellow |
| form | Powder, Crystals and/or Chunks |
| color | White to off-white |
| Water Solubility | soluble |
| Sensitive | Light Sensitive |
| λmax | 248nm |
| BRN | 4093224 |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| Biological Applications | Bacterial vaginosis diagnosis assay; dehydrogenase enzyme assay; diagnostic assay; food and beverage analytes assays; microbial growth assay; detecting bacteria,yeast,fungi,gamma-hydroxybutyric acid (GHB),microbial growth; measuring niacin,bacterial respiratory activity,superoxide dismutase;treating cancer |
| InChI | InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1 |
| InChIKey | JORABGDXCIBAFL-UHFFFAOYSA-M |
| SMILES | [N+]1(=NC(C2C=CC=CC=2)=NN1C1C=CC(I)=CC=1)C1=CC=C([N+]([O-])=O)C=C1.[Cl-] |
| CAS DataBase Reference | 146-68-9(CAS DataBase Reference) |
| EPA Substance Registry System | 2H-Tetrazolium, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-, chloride (146-68-9) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |   GHS02,GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Danger | |||||||||
| Hazard statements | H228-H335 | |||||||||
| Precautionary statements | P210-P240-P241-P261-P271-P280 | |||||||||
| Hazard Codes | Xn,F,Xi | |||||||||
| Risk Statements | 20/21/22-68/20/21/22-36/37/38-11 | |||||||||
| Safety Statements | 36/37-36/37/39-26-22-16 | |||||||||
| RIDADR | 2811 | |||||||||
| WGK Germany | 3 | |||||||||
| F | 3-8-10 | |||||||||
| TSCA | Yes | |||||||||
| HazardClass | 6.1 | |||||||||
| PackingGroup | III | |||||||||
| HS Code | 29339900 | |||||||||
| NFPA 704 |
|
Iodonitrotetrazolium chloride price More Price(20)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich(India) | I8377 | Iodonitrotetrazolium chloride Used in colorimetric assays. | 146-68-9 | 250MG | ₹9723.6 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | I8377 | Iodonitrotetrazolium chloride Used in colorimetric assays. | 146-68-9 | 1G | ₹23665.2 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | I8377 | Iodonitrotetrazolium chloride Used in colorimetric assays. | 146-68-9 | 5G | ₹100455 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | I8377 | Iodonitrotetrazolium chloride Used in colorimetric assays. | 146-68-9 | 25G | ₹454822.5 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | I10406 | Iodonitrotetrazolium chloride 95% | 146-68-9 | 1G | ₹3862.8 | 2022-06-14 | Buy |
Iodonitrotetrazolium chloride Chemical Properties,Uses,Production
Description
Iodonitrotetrazolium (INT) (chloride) is a monotetrazolium salt used as an indicator dye. It is reduced to an insoluble formazan that is used as a vital dye or indicator of cellular redox activity. Reduction commonly results from the activity of dehydrogenases, although non-
Chemical Properties
slightly yellow powder
Uses
Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.
Definition
ChEBI: An organic chloride salt having iodonitrotetrazolium as the counterion.
Safety
Iodonitrotetrazolium chloride could cause skin irritation, serious eye irritation, and may cause respiratory irritation.
Purification Methods
Recrystallise the chloride from H2O, aqueous EtOH or EtOH/Et2O. Alternatively, dissolve it in the minimum volume of EtOH and add Et2O; or dissolve it in hot H2O (charcoal), filter and precipitate it by adding conc HCl. Filter the solid off and dry it at 100o. Its solubility in H2O at 25o is 0.5%, and in hot MeOH/H2O (1:1) it is 5%. [Fox & Atkinson J Am Chem Soc 72 3629 1950, Beilstein 26 III/IV 1776.]
Properties and Applications
INT (Iodonitrotetrazolium chloride), similar to tetrazolium chloride on reduction, produces a red formazan dye that can be used for quantitative redox assays. It is also toxic to prokaryotes.INT is an artificial electron acceptor that can be utilized in a colorimetric assay to determine protein concentration in a solution. It can be reduced by succinate dehydrogenase to furazan, the formation of which can be measured by absorbance at 490 nm. The activity of succinate dehydrogenase is readily observed by the naked eye as the solution turns from colorless to rusty red.
Iodonitrotetrazolium chloride Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | Inquiry |
|---|---|---|---|---|---|
| Arnish Laborates Pvt Ltd | +91 7208475595 | Maharashtra, India | 187 | 58 | Inquiry |
| Neugen Labs | +91-8133254502 +91-8133254502 | Karnataka, India | 195 | 58 | Inquiry |
| TCI Chemicals (India) Pvt. Ltd. | 1800 425 7889 | New Delhi, India | 6778 | 58 | Inquiry |
| CHEMSWORTH | +91-261-2397244 | New Delhi, India | 6707 | 30 | Inquiry |
| Otto Chemie Pvt. Ltd. | +91 9820041841 | Mumbai, India | 5873 | 58 | Inquiry |
| Central Drug House(P) Ltd. | 91-11-49404040 | New Delhi, India | 6160 | 58 | Inquiry |
| LOBA CHEMIE PVT.LTD. | 91-22-6663 6699 | Mumbai, India | 3077 | 58 | Inquiry |
| Triveni chemicals | 08048762458 | New Delhi, India | 6093 | 58 | Inquiry |
| Alfa International | 08068441114Ext 198 | Kolkata, India | 1 | 58 | Inquiry |
| Primarius Custom Synthesis Private Limited | 08048371930Ext 890 | Vadodara, India | 6 | 58 | Inquiry |
| Supplier | Advantage |
|---|---|
| Arnish Laborates Pvt Ltd | 58 |
| Neugen Labs | 58 |
| TCI Chemicals (India) Pvt. Ltd. | 58 |
| CHEMSWORTH | 30 |
| Otto Chemie Pvt. Ltd. | 58 |
| Central Drug House(P) Ltd. | 58 |
| LOBA CHEMIE PVT.LTD. | 58 |
| Triveni chemicals | 58 |
| Alfa International | 58 |
| Primarius Custom Synthesis Private Limited | 58 |