Thiazolyl Blue

- CAS No.
- 298-93-1
- Chemical Name:
- Thiazolyl Blue
- Synonyms
- MTT;THIAZOLYL BLUE TETRAZOLIUM BROMIDE;METHYLTHIAZOLETETRAZOLIUM;METHYLTHIAZOLETETRAZOLIUM (MTT);MTT - CAS 298-93-1 - Calbiochem;THIAZOYL BLUE TETRAZOLIUM BROMIDE;METHYLTHIAZOLYLDIPHENYL-TETRAZOLIUM BROMIDE;3-(4,5-DIMETHYL-2-THIAZOLYL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE;2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2h-tetrazoliubromide;3-(4,5-DIMETHYL-2-THIAZOLYL)-2,5-DIPHENYL-2H-TETRAZOLIUM BROMIDE
- CBNumber:
- CB3383104
- Molecular Formula:
- C18H16BrN5S
- Molecular Weight:
- 414.32
- MOL File:
- 298-93-1.mol
- MSDS File:
- SDS
- Modify Date:
- 2025/1/27 9:38:02
Melting point | 195 °C (dec.) (lit.) |
---|---|
Density | 1.4886 (estimate) |
storage temp. | 2-8°C |
solubility | methanol: 5 mg/mL, clear |
form | powder |
color | Yellow to orange |
Water Solubility | Slightly soluble.Clear yellow solution at 5mg in 1ml of water on vortexing |
λmax | 378 nm, 242 nm |
Sensitive | Light Sensitive |
BRN | 3825277 |
Stability | Hygroscopic |
Biological Applications | Cell viability assay; microbial growth assays; DNA quantification assays; tissue viability assays; detecting enzymes; measuring membrane potential; treating Alzheimer’s disease,asthma,cancer |
InChIKey | AZKSAVLVSZKNRD-UHFFFAOYSA-M |
CAS DataBase Reference | 298-93-1(CAS DataBase Reference) |
EPA Substance Registry System | 2H-Tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide (298-93-1) |
SAFETY
Risk and Safety Statements
Symbol(GHS) | ![]() ![]() GHS07,GHS08 |
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Signal word | Warning | |||||||||
Hazard statements | H315-H319-H335-H341 | |||||||||
Precautionary statements | P201-P202-P261-P302+P352-P305+P351+P338-P308+P313 | |||||||||
Hazard Codes | Xi,T,Xn | |||||||||
Risk Statements | 46-68-36/37/38 | |||||||||
Safety Statements | 22-24/25-53-45-36/37-26 | |||||||||
WGK Germany | 3 | |||||||||
RTECS | XF8060000 | |||||||||
F | 3-8-10 | |||||||||
TSCA | Yes | |||||||||
HS Code | 29341000 | |||||||||
Hazardous Substances Data | 298-93-1(Hazardous Substances Data) | |||||||||
NFPA 704 |
|
Thiazolyl Blue price More Price(31)
Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
---|---|---|---|---|---|---|---|
Sigma-Aldrich(India) | M5655 | Thiazolyl Blue Tetrazolium Bromide powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC) | 298-93-1 | 100MG | ₹4872.9 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | M5655 | Thiazolyl Blue Tetrazolium Bromide powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC) | 298-93-1 | 500MG | ₹11277.6 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | M5655 | Thiazolyl Blue Tetrazolium Bromide powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC) | 298-93-1 | 1G | ₹18725.7 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | M2128 | Thiazolyl Blue Tetrazolium Bromide 98% | 298-93-1 | 100MG | ₹3663 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | M2128 | Thiazolyl Blue Tetrazolium Bromide 98% | 298-93-1 | 250MG | ₹5672.1 | 2022-06-14 | Buy |
Thiazolyl Blue Chemical Properties,Uses,Production
Chemical Properties
Thiazolyl Blue Tetrazolium Blue (MTT) is a water soluble tetrazolium salt yielding a yellowish solution when prepared in media or salt solutions lacking phenol red. Dissolved MTT is converted to an insoluble purple formazan by cleavage of the tetrazolium ring by dehydrogenase enzymes. This water insoluble formazan can be solubilized using isopropanol or other solvents and the dissolved material is measured spectrophotometrically yielding absorbance as a function of concentration of converted dye.
Definition
ChEBI: Thiazolyl Blue is the bromide salt of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium. It has a role as a dye and a colorimetric reagent. It contains a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium.
General Description
Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. It is used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells. As an electron acceptor, it is utilized for studying nicotinamide adenine dinucleotide phosphate (NADP)-dependent dehydrogenases.
Purification Methods
It is recrystallised by dissolving in MeOH containing a few drops of HBr and then adding dry Et2O to complete the crystallisation, wash the needles with Et2O and dry them in a vacuum desiccator over KOH. [Beyer & Pyl Chem Ber 87 1505 1954, Beilstein 27 III/IV 6045.]
Thiazolyl Blue Preparation Products And Raw materials
Raw materials
Preparation Products
Supplier | Tel | Country | ProdList | Advantage | Inquiry |
---|---|---|---|---|---|
Neugen Labs | +91-8133254502 +91-8133254502 | Karnataka, India | 195 | 58 | Inquiry |
SETV ASRV LLP | +91-9731133411 +91-9731133411 | Telangana, India | 437 | 58 | Inquiry |
Spectrochem Private Limited | 08048372608Ext 246 | Maharashtra, India | 1055 | 58 | Inquiry |
Reax Chemicals | 08048372701Ext 416 | Hyderabad, India | 4217 | 58 | Inquiry |
TCI Chemicals (India) Pvt. Ltd. | 1800 425 7889 | New Delhi, India | 6768 | 58 | Inquiry |
Otto Chemie Pvt. Ltd. | +91 9820041841 | Mumbai, India | 5870 | 58 | Inquiry |
Anand Agencies | 91-20-24454597 | Maharashtra, India | 2332 | 58 | Inquiry |
Loba Chemie Pvt Ltd | +91-22-6663 6663 | Mumbai, India | 498 | 58 | Inquiry |
Sisco Research Laboratories Pvt. Ltd. | +91-22-4268 5800 | Mumbai, India | 4315 | 58 | Inquiry |
Neugen Labs | 1--8133254502 | Karnataka, India | 99 | 58 | Inquiry |
Supplier | Advantage |
---|---|
Neugen Labs | 58 |
SETV ASRV LLP | 58 |
Spectrochem Private Limited | 58 |
Reax Chemicals | 58 |
TCI Chemicals (India) Pvt. Ltd. | 58 |
Otto Chemie Pvt. Ltd. | 58 |
Anand Agencies | 58 |
Loba Chemie Pvt Ltd | 58 |
Sisco Research Laboratories Pvt. Ltd. | 58 |
Neugen Labs | 58 |
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