Nitrotetrazolium blue chloride

Nitrotetrazolium blue chloride Properties

Melting point	200°C
Density	1.5521 (rough estimate)
refractive index	1.7350 (estimate)
storage temp.	2-8°C
solubility	methanol: 50 mg/mL, clear, very strongly yellow
form	Powder/Solid
color	Yellow
Water Solubility	Soluble in water, ethanol and methanol.
Sensitive	Light Sensitive
λmax	256 nm
BRN	4115923
Stability	Stable, but store cold.
Biological Applications	Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting
EPA Substance Registry System	2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride (298-83-9)

SAFETY

Risk and Safety Statements

Symbol(GHS)

GHS07,GHS08

Signal word

Danger

Hazard statements

H315-H319-H332-H360D

Precautionary statements

P201-P261-P264-P280-P304+P340+P312-P308+P313

Hazard Codes

Xn,Xi

Risk Statements

22-68/20/21/22-20/21/22-36-36/37/38-41

Safety Statements

36-45-36/37-26-39

WGK Germany

3

RTECS

XF8045000

F

8-10-23

TSCA

Yes

HS Code

32041900

Toxicity

LD50 orl-mus: 2 g/kg YAKUD5 32,614,1990

NFPA 704

	1
2		0

Nitrotetrazolium blue chloride price More Price(29)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich(India)	N6876	Nitrotetrazolium Blue chloride ≥90.0% (HPLC)	298-83-9	50MG	₹4481.55	2022-06-14	Buy
Sigma-Aldrich(India)	N6876	Nitrotetrazolium Blue chloride ≥90.0% (HPLC)	298-83-9	100MG	₹6495	2022-06-14	Buy
Sigma-Aldrich(India)	N6876	Nitrotetrazolium Blue chloride ≥90.0% (HPLC)	298-83-9	250MG	₹10435.3	2022-06-14	Buy
Sigma-Aldrich(India)	N6876	Nitrotetrazolium Blue chloride ≥90.0% (HPLC)	298-83-9	10X10MG	₹13747.75	2022-06-14	Buy
Sigma-Aldrich(India)	N6876	Nitrotetrazolium Blue chloride ≥90.0% (HPLC)	298-83-9	500MG	₹20166.98	2022-06-14	Buy

Product number	Packaging	Price	Buy
N6876	50MG	₹4481.55	Buy
N6876	100MG	₹6495	Buy
N6876	250MG	₹10435.3	Buy
N6876	10X10MG	₹13747.75	Buy
N6876	500MG	₹20166.98	Buy

Nitrotetrazolium blue chloride Chemical Properties,Uses,Production

Description

Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.

Chemical Properties

NBT is a yellow crystals that can soluble in water, aqueous buffers, and dimethylformamide. Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology.

Uses

Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.

Definition

ChEBI: Nitro blue tetrazolium dichloride is an organic chloride salt having nitro blue tetrazolium(2+) as the counterion. It contains a nitro blue tetrazolium(2+).

Application

Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
It has been used as a substrate for western blot detection.
It has been used for the staining of histological tissue sections.
Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease.
It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase.
Further, it is used in immunology for detection of alkaline phosphatase.

General Description

Nitrotetrazolium Blue chloride (NBT) is a colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

Hazard

Moderately toxic by ingestion.

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.

Safety Profile

Moderately toxic by ingestion. When heated to decomposition it emits toxic vapors of NOx and Clí.

Nitrotetrazolium blue chloride Preparation Products And Raw materials

Raw materials

3,3'-Dimethoxybenzidine dihydrochloride Isoamyl nitrite Ethanol Benzaldehyde Tetrahydrofuran

4-Nitrophenylhydrazine

chevron_left

1of2

chevron_right

Preparation Products

Nitrotetrazolium blue chloride Suppliers

Global( 441)Suppliers

Supplier	Tel	Country	ProdList	Advantage	Inquiry
GLR Innovations	+91 9891111994	New Delhi, India	4542	58	Inquiry
Arnish Laborates Pvt Ltd	+91 7208475595	Maharashtra, India	187	58	Inquiry
Neugen Labs	+91-8133254502 +91-8133254502	Karnataka, India	195	58	Inquiry
S D Fine Chem Limited	+91-9323715856 +91-9323715856	Maharashtra, India	368	58	Inquiry
Oxford laboratories	+91 250 239 0032	Maharashtra, India	652	58	Inquiry
Kiran Lights Laboratories	91-9987701501	Maharashtra, India	262	58	Inquiry
Oceanic Pharmachem Pvt. Ltd.	91-22-42128600	Maharashtra, India	2006	58	Inquiry
Anand Agencies	91-20-24454597	Maharashtra, India	2337	58	Inquiry
Brisben Chemicals	08048963750	Mumbai, India	509	58	Inquiry
Sisco Research Laboratories Pvt. Ltd.	+91-22-4268 5800	Mumbai, India	4317	58	Inquiry

Supplier	Advantage
GLR Innovations	58
Arnish Laborates Pvt Ltd	58
Neugen Labs	58
S D Fine Chem Limited	58
Oxford laboratories	58
Kiran Lights Laboratories	58
Oceanic Pharmachem Pvt. Ltd.	58
Anand Agencies	58
Brisben Chemicals	58
Sisco Research Laboratories Pvt. Ltd.	58

Nitrotetrazolium blue chloride Spectrum

Nitrotetrazolium blue chloride(298-83-9)¹HNMR Nitrotetrazolium blue chloride(298-83-9)IR1 Nitrotetrazolium blue chloride(298-83-9)IR2

298-83-9(Nitrotetrazolium blue chloride)Related Search:

1H-Tetrazole-1-acetic acid 2,3,5-Triphenyltetrazolium chloride Benzyl chloride Calcium chloride Mepiquat chloride

Polyvinyl chloride Ammonium chloride Tetrazole Choline chloride Sodium chloride

Tetrazolium Blue Chloride 4-Chloronitrobenzene 4-Nitrobenzyl chloride Tetranitroblue tetrazolium chloride Methylene Chloride

M-NITRO CHLORO BENZENE(MNCB) p-Nitro blue tetrazolium, diformazan p-NBT, diformazan TETRAZOLE BLUE DIFORMAZAN Nitro Blue Tetrazolium Chloride

chevron_left

1of4

chevron_right

2h-tetrazolium,3,3’-(3,3’-dimethoxy[1,1’-biphenyl]-4,4’-diyl)bis[2-(4-nitrophe nbt(dye) nitrobluetetrazoliumsalt nitrotetrazoliumbt nitrotetrazoliumchlorideblue tetrazoliumnitroblue tetrazoliumnitrobt 2H-TETRAZOLIUM,2,2'-((3,3')-DIMETHOXY[1,1'-BIPHENYL]-4,4'-DIYL)BIS[3-(4-NITROPHENYL)-5-PHENYL-],DICHLORIDE 3,3'-(3,3'-DIMETHOXY[1,1'-BIPHENYL]-4,4'-DIYL)BIS[2-(4-NITROPHENYL)]-5-PHENYL-2H-TETRAZOLIUM DICHLORIDE 3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLENE)BIS[2-(4-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM CHLORIDE] 3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLENE)-BIS-(2-P-NITROPHENYL)-5-(PHENYL)-2H-TETRAZOLIUM CHLORIDE 3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLENE)BIS[2-(P-NITROPHENYL)-5-PHENYLTETRAZOLIUM] CHLORIDE 3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLYLENE)BIS[2-(P-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM CHLORIDE] 2,2'-DI-4-NITROPHENYL-5,5'-DIPHENYL-3,3'-(3,3'-DIMETHOXY-4,4'-DIPHENYLENE)-DITETRAZOLIUM-CHLORIDE 2,2'-BIS(4-NITROPHENYL)-5,5'-DIPHENYL-3,3'-(3,3'-DIMETHOXY-4,4'-DIPHENYLENE)DITETRAZOLIUM CHLORIDE 2,2'-DI-P-NITROPHENYL-5,5'-DIPHENYL-(3,3'-DIMETHOXY)-4,4'-BISPHENYLENEDITETRAZOLIUM CHLORIDE 2,2'-DI-P-NITROPHENYL-5,5'DIPHENYL-[3,3'-DIMETHOXY-4,4'-DIPHENYLENE] DITETRAZOLIUM CHLORIDE DITETRAZOLIUM DYE P-NITROBLUETETRAZOLIUMCHLORIDE,CRYSTAL,REAGENT NITROBLUETETRAZOLIN Nitro BT (Nitro blue tetrazolium chloride) NITRO BLUE TETRAZOLIUM CHLORIDE MONOHYDRATE extrapure AR p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT, 3,3μ-(3,3μ-Dimethoxy-4,4μ-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,2μ-bis(4-Nitrophenyl)-5,5μ-diphenyl-3,3μ-(3,3μ-dimethoxy-4,4μ-diphenylene)ditetrazolium chloride NBT Vials p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT, Nitrotetrazolium Blue chloride, 3,3μ-(3,3μ-Dimethoxy-4,4μ-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,2μ-bis(4-Nitrophenyl)-5,5μ-diphenyl-3,3μ-(3,3μ-dimethoxy-4,4μ-diphenylene)ditetrazolium chloride 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, chloride (1:2) 3,3'-[3,3-Dimethoxy-(1,1-biphenyl)-4,4-diyl]-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchloride] NBTMF Nitro blue tetrazolium >99% Nitro Blue Tetrazolium chloride, tech. 90% Nitro Blue Tetrazolium [for Biochemical Research] Nitro Blue Tetrazolium Chloride monohydrate,90% Nitro Blue Tetrazolium Chloride monohydrate,85% NITRO BLUE TETRAZOLIUM CHLORIDE MONOHYDRATE: TECH., 90% 2H-Tetrazolium, 3,3'-(3,3'-dimethoxy[1,1'- biphenyl]-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl -, dichloride 2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy- p-Nitro-Blue tetrazolium chlo Nitrotetrazolium Blue chloride ,90% 2-(3,3'-Dimethoxy-4'-(2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium-3-yl)-[1,1'-biphenyl]-4-yl) p-Nitro-Blue tetrazolium chlori 2h-tetrazolium,3,3’-(3,3’-dimethoxy(1,1’-biphenyl)-4,4’-diyl)bis(2-(4-nitrophe 2h-tetrazolium, 2,20-(3,30-dimethoxy [1,10-biphenyl]-4,40-diyl)bis[3-(4-nitrophenyl)-5-phenyl]-, dichloride 2h-tetrazolium, 3,30-(3,30-dimethoxy- 4,40-biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl]-, dichloride Tetrazolium, 3,30-(3,30-dimethoxy-4,40-biphenylylene) bis[2-(p-nitrophenyl)-5-phenyl-2h-chloride] 2, 20-bis(p-nitrophenyl)-5,50-diphenyl-3,30-(3,30-dimethoxy- 4,40-biphenylylene)ditetrazolium Chloride 2,20-bis(p-nitrophenyl)- 5,50-diphenyl-3,30-(3,30-dimethoxy-4,40-diphenylene) ditetrazolium Chloride 2,20-di-p-nitrophenyl- 5,50-diphenyl-3,30-bis(3,30-dimethoxy-4,40-biphenylene) ditetrazolium Chloride 2,20-dinitrophenyl-5,50-diphenyl- 3,30-dimethoxy-4,40-diphenylene)ditetrazolium Chloride 3,30-(3,30-dimethoxy-4,40-biphenylylene)bis[2-(p-nitrophenyl)- 5-phenyl-2h-tetrazolium Chloride] 3,30-(3,30- dimethoxy-4,40-diphenylene)bis[2-(p-nitrophenyl)-5- phenyltetrazolium Chloride] Nsc 27622 P- nitrotetrazolium Blue Nitro Blue Tetrazolium (nbt) NBT CHLORIDE NITRO BLUE TETRAZOLIUM (NBT) REDUCTION NITRO TETRAZOLIUM BLUE NITROTETRAZOLIUM BLUE CHLORIDE P-NITRO-BLUE TETRAZOLIUM CHLORIDE P-NITROBLUE TETRAZOLIUM DICHLORIDE