KANAMYCIN manufacturers and suppliers in india

KANAMYCIN

Properties

Melting point:: >175°C (dec.)

alpha: D24 +146° (0.1N H2SO4)

Boiling point:: 581.13°C (rough estimate)

Density: 1.4042 (rough estimate)

refractive index: 1.6700 (estimate)

storage temp.: 2-8°C

solubility: Methanol (Slightly, Sonicated), Water (Slightly)

pka: pKa 6.40/7.55/8.40/9.40(H2O) (Uncertain)

form: liquid

color: White to Off-White

EPA Substance Registry System: D-Streptamine, O-3-amino-3-deoxy-.alpha.-D-glucopyranosyl-(1.fwdarw.6)-O-[6-amino-6-deoxy-.alpha.-D-glucopyranosyl-(1.fwdarw.4)]-2-deoxy- (59-01-8)

Safety Information

WGK Germany: 2

Hazardous Substances Data: 59-01-8(Hazardous Substances Data)

Toxicity: LD50 i.v. in mice: 583 mg/kg (Wakazawa)

Use

Kanamycin is a well-known bactericidal antibiotic. It belongs to the aminoglycoside antibiotic group. It can be used for the treatment of various pathogens including E. coli, Proteus species (both indole-positive and indole-negative), Enterobacter aerogenes, Klebsiella pneumoniae, Serratia marcescens and Acinetobacter species1-4. Kanamycin is isolated from the bacterium Streptomyces kanamyceticus and its most commonly used form is kanamycin sulfate5. Aminoglycoside-type drug take actions through irreversibly binding to the 30S subunit of the ribosome, further blocking the protein synthesis. Kanamycin kills bacteria cells binds to four nucleotides of 16S rRNA and a single amino acid of protein S12. This interferes with decoding site in the vicinity of nucleotide 1400 in 16S rRNA of 30S subunit. This region interacts with the wobble base in the anticodon of tRNA. This leads to interference with the initiation complex, misreading of mRNA so incorrect amino acids are inserted into the polypeptide leading to nonfunctional or toxic peptides and the breakup of polysomes into nonfunctional monosomes5.